Anders Retzius and the Dental Histologists of the Mid-Nineteenth Century: Their Contribution to Comparative Anatomy, Histology and Anthropology.

Anatomy, comparative anatomy and embryology are fundamental to taxonomy and evolutionary biology. In the mid-nineteenth century many anatomists and zoologists made major contributions to more than one of these disciplines and a surprising number of them were also histologists. Historical accounts of discoveries and developments in anatomy, and in particular dental histology, rarely consider broader contributions and have tended to be concerned with establishing historical priority about who discovered or described what first. The period 1830 to 1840 saw new developments in light microscopy that enabled studies of histology, cellular pathology and embryology. It also saw a shift away from older ideas such as Naturphilosophie and vitalism towards a more rigorous experimental approach to scientific investigation. Many scientists with diverse research interests were working in parallel on comparative dental histology and were in many cases largely unaware of each other's work. One researcher, Anders Retzius, travelled widely across Europe, corresponded regularly with his scientific colleagues and, probably unbeknownst to himself in his own lifetime, made a lasting contribution to dental histology. Anders Retzius was a clinician, an anatomist, a comparative anatomist, a histologist and latterly an anthropologist. His life and career spanned the whole of this fast-moving period in the history of anatomy and histology.

Distinguishing the Chinese materia medica Tiepishihu from similar Dendrobium species of the same genus using histological and microscopic method.

The Chinese Materia Medica, Tiepishihu, used as a tonic for over one thousand years, is a well-known precious medicine in China. According to the Chinese Pharmacopoeia, its source is the species Dendrobium officinale Kimura et Migo, which is distinguished from other species in Dendrobium genus. However, these species from the same genus are similar with Tiepishihu and caused confusion in the market. To find a quick and simple method to distinguish Tiepishihu from other similar species, histologic and microscopic methods were combined together to investigate the transverse section of stem of Tiepishihu and other similar species. Phloroglucinol test solution with hydrochloric acid was used to reveal the lignified tissue by staining the transverse section of Tiepishihu and similar species. Results revealed the unique identification characteristics to distinguish Tiepishihu from similar species, which were difficult to distinguish by other methods. The identification characteristics of Tiepishihu include the cells of vascular bundle sheath were stained red, parenchyma cells were not stained red. What's more, other species can be distinguished from each other with microscopic and histological characteristics. These characteristics proved stable and can be easily observed by normal light microscopic examination. This method is rapid, accurate, stable, and inexpensive.

Colitis ulcerosa en un lactante / Ulcerative colitis in an infant

Se describe una paciente de 6 meses de edad con colitis ulcerosa. Las manifestaciones clínicas consistían en deposiciones sanguinolentas, fiebre, pérdida de peso, anemia significativa, leucocitosis, velocidad de eritrosedimentación globular aumentada e hipoalbuminemia. Los estudios bacteriológicos y copropararitológicos seriados descartaron infección. El enema baritado precipitó deterioro clínico de la paciente, requiriendo intervención quirúrgica. El examen histológico reveló abscesos de las criptas, pseudopólipos, distorsión de las criptas y disminución de las células caliciformes. El tratamiento con sulfasalazina y el manejo nutricional ha sido exitoso