[Determination of four kinds of purines in deer fetus soft capsule by HPLC].

OBJECTIVE: To establish an HPLC method for simultaneous determination of four kinds of purines in deer fetus soft capsule. METHODS: Four kinds of purines were detected and determined by HPLC. The mobile phase was 0.02 mol/L KH2PO4 (containing 1 mmol/L heptane sulfonic acid sodium, pH = 3.8)-methanol = 97:3. Detection wavelength was 254 nm and flow rate was 1.5 ml min. The linear relationship of four kinds of purines was as follows: hypoxanthine: Y1 = 83695X1 + 355 (r1 = 0.9998), with the linear range 0.040-0.667 mg/mL; xanthine: Y2 = 50638X2 + 39 (r2 = 0.9989), with the linear range 0.008-0.119 mg/mL; guanine: Y3 = 30269X3-9562 (r3 = 0.9924), with the linear range 0.018 - 0.279 mg/mL; adenine: Y4 = 38975X4-8671 (r4 = 0.9989), with the linear range 0.027-0.399 mg/mL The average sample recovery rate of hypoxanthine, xanthine, guanine and adenine were 98.1%, 98.6%, 98.0% and 97.5%, with RSD 1.0%, 0.4%, 0.8% and 0.6%, respectively. RESULTS: The content of hypoxanthine, xanthine, guanine and adenine in 3 lots of deer fetus soft capsule were 116.5-132.0 microg/capsule, 21.2-23.0 microg/capsule, 48.6-54.3 microg/capsule and 68.9-75.2 microg/capsule, respectively. CONCLUSIONS: This method is simple,accurate and reproducible, which provides a basis for quality control of purines in deer fetus soft capsule.

[Determination of capsaicin in Pheretima aspergillum from different habitats by RP-HPLC].

OBJECTIVE: To determine the content of hypoxanthine in Pheretima aspergillum from different habitats. METHOD: A RP-HPLC method was established. The chromatographic column was Inertsil ODS-EP. The mobile phase was H2O-CH3OH-C4H8O(93:: 7: 0.05). The flow rate was 1.0 ml/min, and the detection wavelength was 254 nm. RESULTS: The average recoveries for hypoxanthine was 98.6% , precision of the method was 0. 50% (RSD, n = 6). CONCLUSION: The method can be used to determine the content of hypoxanthine in Pheretima aspergillum from diffrent habitats.

[Determination of hypoxanthine and xanthine in hippocampus by HPLC method].

OBJECTIVE: A method was established for the determination of hypoxanthine and xanthine in Hippocampus by HPLC. METHODS: The HPLC system consisted of a Kromasil C18 column (250 mm x 4.6 mm, 5 microm) with methanol-water (2:98, V/V) as the mobile phase. The wavelength of detection was 254 nm. RESULTS: The linear range of hypoxanthine and xanthine were 29.0-464.0 ng (r = 0.99996) and 24.5-392.0 ng (r = 0.9995) respectively. The average recovery of hypoxanthine was 100.73%, RSD = 1.31% (n = 6). The average recovery of xanthine was 100.54%, RSD = 1.14% (n = 6). CONCLUSION: This method is convenient and the result is accurate. It can be used for quality control of Hippocampus.

[HPLC determination of uracil xanthine, hypoxanthine and uridine in species of animal drugs].

OBJECTIVE: To make further studies on the water-soluble components in animal drugs by determining the contents of uracil, xanthine, hypoxanthine and uridine from ten species of animal drugs. METHOD: The determination was affected by RP-HPLC, using Shim-pack CLC-ODS column(150 mm x 6 mm), 0.05 mol/L dibasic ammonium phosphate solution as mobile phase, flow rate 0.8 ml/min and detection at 254 nm. RESULTS: This method is accurate, rapid and reproducible. Analytical data for Hirudo nipponia, Eupolyphaga sinensis, Scolopendra subspinipes mutilans, Buthus martensii, Bombyx mori, Mylabris cicorri, Aspongopus chinensis, Lytta caraganae, Tabanus bivittatus and Huechys sanguinea are given. CONCLUSION: There are marked discrepancies in the contents of the four components as shown on the chromatograms, a point that may be helpful in identifying the above-cited ten species of animal drugs.

[The comparison of contents of uracil, xanthine and hypoxanthine in five species of leech].

Three constituents(uracil, xanthine, hypoxanthine) were isolated from five species of leech and determined by reversed phase HPLC. The column employed was Shim-pack CLC-ODS C18(150 mm x 6 mm). The mobile phase was 0.05 mol/l ammonium phophate dibasic solution(pH = 8.4). The flow rate was 0.8 ml/min and detection was effected at 254 nm. This method is accurate, rapid and reproducible. Analytical data for five species and Whitmania pigra samples from different places were given.