ABSTRACT
BACKGROUND: Frog skin has been sequentially and scientifically evaluated by our group for its wound healing efficiency. Owing to the complex structure of skin, attempts were being made to analyse the role of individual constituents in different phases of healing. Our earlier papers have shown the significance of frog skin not only in wound healing but also enhancing the proliferating activity of the epidermal and dermal cells which are instrumental for normal healing process. We also have identified for the first time novel antimicrobial peptides from the skin of Rana tigerina and thereby reduce the complications involved in the sepsis. PURPOSE OF THE STUDY AND RESULTS: The current study envisages the role of frog skin lipids in the inflammatory phase of wound healing. The lipid moiety of the frog skin dominated by phospholipids exhibited a dose dependent acceleration of healing irrespective of the mode of application. The efficiency of the extract is attributed partially to the anti-inflammatory activity as observed by the histochemical and immunostimulatory together with plethysmographic studies. CONCLUSIONS: Thus, frog skin for the first time has been demonstrated to possess lipid components with pharmaceutical and therapeutic potential. The identification and characterization of such natural healing molecules and evaluating their mechanism of action would therefore provide basis for understanding the cues of Nature and hence can be used for application in medicine.
Subject(s)
Anti-Inflammatory Agents, Non-Steroidal/therapeutic use , Lipids/therapeutic use , Materia Medica , Ranidae , Skin/chemistry , Skin/drug effects , Tissue Extracts/therapeutic use , Wound Healing/drug effects , Administration, Cutaneous , Animals , Anti-Inflammatory Agents, Non-Steroidal/administration & dosage , Anti-Inflammatory Agents, Non-Steroidal/analysis , Anti-Inflammatory Agents, Non-Steroidal/immunology , Dose-Response Relationship, Drug , Drug Discovery , Edema/chemically induced , Edema/drug therapy , Female , Granulation Tissue/chemistry , Granulation Tissue/drug effects , Hypersensitivity/drug therapy , Hypersensitivity/immunology , Immunity, Humoral/drug effects , India , Injections, Intraperitoneal , Lipids/administration & dosage , Lipids/analysis , Lipids/immunology , Medicine, Traditional , Rats , Rats, Wistar , Skin/injuries , Tissue Extracts/administration & dosage , Tissue Extracts/chemistry , Tissue Extracts/immunologyABSTRACT
A sensitive and specific method with high performance liquid chromatographic fingerprints was developed for controlling the quality of donkey-hide glue and tortoise-shell glue according to the liposoluble constituents. The samples were prepared with the liquid-liquid-liquid three-phase static extraction. The liposoluble constituents were analysed by reversed-phase high performance liquid chromatography with a gradient program and detected at 205 nm. The chromatograms were evaluated by similarity and cluster analysis. The common peaks of two glues were marked respectively. The differences between two glues were revealed by similarity and cluster analysis. The method has good repeatability and stability, and can be served as the means to identify different kinds of animal glue.