ABSTRACT
Kangfuxin liquid (KFX), an extract of the American cockroach, has been clinically proven to be effective in various skin damage disorders, but there are no reports on its use in photodamage. We explored the effect of KFX on ultraviolet B (UVB)-induced photodamage and whether its mechanism was related to autophagy. We found that KFX treatment reduced UVB-induced reactive oxygen species production and improved the vitality of cells inhibited by UVB irradiation. The expression of LC3 (A/B), which was inhibited after UVB irradiation, could be rescued by KFX treatment. Furthermore, KFX may upregulate the level of cellular autophagy by regulating the AMPK-mTOR signaling pathway. When the autophagy inhibitor wortmannin was used to inhibit autophagy, the protective effect of KFX on cells was diminished or even disappeared. Our study suggests that KFX may resist UVB-mediated oxidative stress damage of HaCaT through the induction of autophagy.
Subject(s)
HaCaT Cells , Materia Medica , Humans , Materia Medica/pharmacology , Autophagy , Oxidative Stress , Reactive Oxygen Species , Ultraviolet Rays/adverse effects , KeratinocytesABSTRACT
AIM: To investigate the effect of polypeptide from Chlamys farreri (PCF) on ultraviolet B (UVB)-induced apoptosis and DNA damage in cultured normal human dermal fibroblasts. METHODS: MTT assay was used to measure the viability of cells. Measurements of apoptosis and cytosolic free [Ca2+]i were performed with flow cytometry. The comet assay was employed to detect DNA damage in individual cell. RESULTS: PCF (0.25 %-1%) greatly enhanced the proliferative capacity of cultured fibroblasts irradiated by UVB (1.176 x 10(-4) J/cm2) and markedly reduced apoptosis and the level of DNA damage in a concentration-dependent manner. Meanwhile, PCF could decrease the cytosolic free [Ca2+]i (P<0.01, compared with UVB model). CONCLUSION: The inhibitory effect of PCF on UVB-induced photoaging is due to enhanced abating of UVB-injured DNA and UVB-induced apoptosis. Therefore, PCF can resist UV-induced aging development at the initiation stage.
Subject(s)
Apoptosis/drug effects , DNA Damage/drug effects , Ostreidae , Peptides/pharmacology , Ultraviolet Rays/adverse effects , Animals , Apoptosis/radiation effects , DNA Damage/radiation effects , Dermis/cytology , Fibroblasts/cytology , Fibroblasts/drug effects , Fibroblasts/radiation effects , Humans , Materia Medica/isolation & purification , Materia Medica/pharmacology , Ostreidae/chemistry , Peptides/isolation & purification , Protective Agents/isolation & purification , Protective Agents/pharmacology , Random AllocationABSTRACT
No tratamento de quadros dermatologicos despertou-nos a atencao o bom resultado obtido nos casos de fotossensibilidade de variada etiologia; estes quadros, sem solucao na terapeutica academica, tem conseguido melhora e regressao definitiva dentro da lei dos semelhantes. Tem sido notavel a frequencia da indicacao de APIS MELLIFICA; posteriormente encontramos apoio num trabalho experimental de BASTIDE e COL. comprovando em cobaias o efeito anti-inflamatorio de APIS MELLIFICA, quando os animais sao submetidos a acao agressiva dos raios ultra-violetas