RESUMEN
OBJECTIVE: To compare eighteen samples of Forest frog's oviduct from different regions of northeast China, in order to fomulate the quality evaluation standard. METHODS: According to the documents, comparing the target constituent of Forest frog's oviduct, including the mositure, ash, protein, lipid and expansibility were analysed. RESULTS: It was similar to the chemical constituent in Forest frog's oviduct from different habitiat of northeast China. CONCLUSION: The germplasm of this species is stable.
Asunto(s)
Materia Medica/química , Oviductos/química , Rana esculenta/metabolismo , Animales , China , Ecosistema , Femenino , Lípidos/análisis , Materia Medica/análisis , Materia Medica/normas , Proteínas/análisis , Control de Calidad , Rana esculenta/clasificación , Rana esculenta/crecimiento & desarrolloRESUMEN
OBJECTIVE: To develop a new method for the determination of cholesteryl palmitate in Oviductus Ranae. METHOD: A HPLC method was set up, using Zorbax Silica column and cyclohexane-diethyl ether (40:1) as mobile phase with a flow rate of 1.0 mL x min(-1), and the UV detection wavelength was 203 nm. RESULT: The calibration curve was linear over the range of 0.60-8.92 microg (r = 0.9997), the average recovery of the method was 98.4%. RSD 1.8% (n = 6). CONCLUSION: The results showed that method was reliable and accurate.
Asunto(s)
Ésteres del Colesterol/análisis , Materia Medica/análisis , Oviductos/química , Rana temporaria , Animales , Cromatografía Líquida de Alta Presión/métodos , Femenino , Materia Medica/química , Control de CalidadRESUMEN
OBJECTIVE: Studies on the substance with nourishment Yin for a reasonable and rational auality appraise for turtleback. METHOD: To separate by chromatography and identifying with MS, 1H NMR, 13C NMR, IR. RESULT: Two compounds were separated and identified as hexadecanoyl cholesterol ester and cholesterol. CONCLUSION: The two compounds are isolated from turtleback for the first time.
Asunto(s)
Ésteres del Colesterol/aislamiento & purificación , Materia Medica/química , Tortugas , Animales , Colesterol/aislamiento & purificación , Ésteres del Colesterol/químicaRESUMEN
OBJECTIVE: To determine the origin of Rana temporaria for quality Oviduetus Ranae in the light of historical documents and modern researches on the classification of Rana temporaria chensinensis. METHOD: Works of Chinese meteria medica of all ages, related historical documents and reports from home and abroad on researches of R. temporaria chensinensis were consulted, sorted out, analyzed and summarized. RESULT: The original Shange recorded in the works of Chinese meteria medica is R. temporaria chensinensis, which is the independent species, not one of species of European forest frogs. R. temporaria chensinensis is divided into 4 subspecies: R. temporaria chensinensis, Lanzhou, Kangding, and Changbaishan. The origin of R. temporaria is Changbaishan subspecies of R. temporaria chensinensis. CONCLUSION: Changbaishan subspecies of R. temporaria chensinensis is determined as the origin for quality Oviduetus Ranae.