RESUMEN
OBJECTIVE: To investigate the proliferation inhibition and the differentiation effects of realgar (As4S4) nano-particles on human acute myeloid leukemia cell line HL-60. METHOD: Cell viability was determined by MTT and PI-stained cell cycle assays. The realgar induced morphological changes on cells were examined after Wright-Giemsa staining. The cell differentiation was evaluated with NBT and specific cell surface antigen (CD11b and CD14) expression assays. RESULT: HL-60 cells exhibited obvious morphological features of differentiation after the realgar treatment. A 24 h incubation of the cells with 0.25-1.0 micromol x L(-1) realgar caused a great increase in NBT reduction ability. The expressions of CD11b and CD14 were augmented in cells treated with 0.50 micromol x L(-1) realgar for 48 h, and cell cycles were arrested in G1 phase. CONCLUSION: Low dose realgar induces differentiation in human acute myeloid leukemia cell line HL-60.
Asunto(s)
Antineoplásicos/administración & dosificación , Arsenicales/administración & dosificación , Diferenciación Celular/efectos de los fármacos , Materia Medica/administración & dosificación , Sulfuros/administración & dosificación , Antineoplásicos/farmacología , Arsenicales/farmacología , Antígeno CD11b/metabolismo , Proliferación Celular/efectos de los fármacos , Fase G1/efectos de los fármacos , Células HL-60 , Humanos , Receptores de Lipopolisacáridos/metabolismo , Materia Medica/farmacología , Nanopartículas , Sulfuros/farmacologíaRESUMEN
Some of traditional Chinese medicine (TCM) contain arsenide, such as realgar. The total amount of arsenic in the TCM exceeds the limits according to related regulations. But the roles of arsenic in TCM or its side-effects depend on its species existed in those therapies, not the total amount of arsenic. Therefore, in recent years, the analysis of arsenic in TCM focuses on the species of arsenic. The present paper summarized some methods and techniques in the speciation analysis of arsenic in TCM, in order that optimal methods can be chosen and the roles of arsenic could be evaluated properly.