The liquid Kangfuxin (KFX) has efficient antifungal activity and can be used in the treatment of vulvovaginal candidiasis in mice.

Vulvovaginal candidiasis (VVC) is an infectious disease caused mainly by Candida albicans. Kangfuxin (KFX) is a traditional Chinese medicine preparation made from Periplaneta americana extracts, which promotes wound healing and enhances body immunity and also acts as an antifungal agent. Here, we evaluated the effect of KFX in the treatment of VVC in vitro and in vivo. The minimum inhibitory concentration (MIC50 ) of KFX against C. albicans ranged from 7·65 to 20·57%. In addition, KFX was more efficient than fluconazole (FLC) in inhibiting the drug-resistant C. albicans, and the effect was more intense after 8 h. The KFX treatment also exhibited good activity in vivo. It restored the body weight and reduced the vulvovaginal symptoms in mice induced with VVC. It downregulated the expression of the hyphae-related gene, HWP1, thus inhibiting the growth and development of C. albicans hyphae. It also increased the number of neutrophils and promoted the secretion of interleukin-17A (IL-17A); however, the levels of interleukin-8 (IL-8) and interleukin-1ß (IL-1ß) decreased in mice with VVC. We deduce that KFX effectively treats vaginal candidiasis in two ways: by inhibiting the growth and development of mycelia to reduce colonization of C. albicans and by promoting the secretion and release of IL-17A and neutrophils in high numbers to fight C. albicans infection. This study provides a theoretical basis for the use of KFX for the clinical treatment of VVC.

[An exploration on the factors of Jiao Xun's compilation and edit of Wu shi ben cao (Wu's Materia Medica)].

Wu shi ben cao (Wu's Materia Medica) written by Wu Pu was long lost. Jiao Xun of the Qing Dynasty was the first to compile and edit this book. The background of Jiao's work included: ① the necessary for revising Mao shi niao shou cao mu chong yu shi (Annotation to the Bird, Beast, Grass, Wood, Insect and Fish in Mao's Book of Songs); ② fond of medicine; ③ the feeling of retaining hometown documents; ④ the influence of the traditional style of compilation and edit; whereas Jiao's intrinsic characteristics included: ① a wealth of books and convenient borrowing environment; ② the habit of reading and transcription; ③ the thought of pu xue (the School of ancient textual criticism), that is "using the Confucian's method to study medical classics" ; ④ the methods of studying classics based on the classical texts; ⑤ high literate level of compilation of losing texts of classics. The original texts of Wu shi ben cao was thus resumed preliminarily by Jiao Xun's work.

The safety and value of naloxone as a therapeutic aid.

The safety and value of naloxone as a therapeutic aid was demonstrated in a large population of narcotic dependent persons over a two-year period. Naloxone was used to precipitate the narcotic withdrawal syndrome. This withdrawal syndrome was rated according to a previously developed scale. Retrospectively, naloxone rating scores were correlated with the patients' initial dose of methadone. With patients who received 0.8 mg naloxone, good correlation was obtained between the naloxone test score and the optimum methadone dose (mg), as indicated by the patients' physical status during the initial three days of methadone treatment. Three hundred and sixty-three tests were administered to 343 persons and no individuals developed any serious effects such as convulsions, syncope or cardiovascular collapse.

[Pharmacological study on magnetite].

Magnetite can markedly inhibit the rodent turn-around reaction induced by acetic acid, reduce the threshold dose of pentobarbital sodium and shorten rodent's incubation period of falling asleep. It has also the following effects; antagonizing metrazol which causes rodent convulsions, postponing the incubation period of being startled by Huisuling, cutting down the extent of rodent's foot swells caused by JCCJ, and diminishing bleeding time and congulating time.