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1.
J Altern Complement Med ; 9(5): 719-25, 2003 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-14629849

RESUMO

OBJECTIVES: The primary biomolecular target of a homeopathic potency is unknown. If it is a plasma membrane protein such as water-channel protein, the drug would alter water permeation in cells. Therefore, the objective is to see if potentized homeopathic drugs like Mercuric chloride 30c and Nux vomica 30c could alter permeation of water through the erythrocytes of a fresh water fish under acute ethanol intoxication. LOCATION: The work was carried out in the Zoology Laboratory of Visva Bharati University, Santiniketan, West Bengal, India. SUBJECT: Live freshwater catfish. DESIGN: Erythrocytes collected from fish with and without ethanol intoxication were incubated in distilled water at 30 degrees C for 30 minutes with Ethanol 30c (control), Merc cor 30c (test 1), and Nux vomica 30c (test 2). Merc cor 30c and Nux vom 30c were prepared by successive dilution of the respective mother tinctures with 90% ethanol (1:100) followed by sonication at 20 kHz for 30 seconds in 30 steps. Ethanol 30c was prepared in the same way from 90% ethanol diluted with 90% ethanol. In another experiment, fish were pretreated with Ethanol 30c and Nux vom 30c followed by ethanol injection at 2 g/kg of body weight. Then their erythrocytes were tested in vitro with the same potencies. After centrifugation of blood samples, fluid part was removed, erythrocyte pellets dried in a BioChemical Oxygen Demand (BOD; Atlas Surgical, New Delhi, India) incubator at 90 degrees C for 12 hours and intracellular water content measured. RESULTS: Red blood cells (RBCs) from ethanol-injected fish permeated more water than those from normal fish. Water permeation was enhanced with Merc cor 30c and Nux vom 30c. RBCs from fish pretreated with Nux vom 30c imbibed more water in in vitro treatments than those from fish pretreated with Ethanol 30c. CONCLUSION: Because water channel proteins or aquaporins are mainly responsible for water transport through the plasma membrane of RBCs, it is thought that potentized drugs interact with these proteins, thereby facilitating water influx in the cells.


Assuntos
Intoxicação Alcoólica/metabolismo , Eritrócitos/efeitos dos fármacos , Água Doce/química , Homeopatia/métodos , Cloreto de Mercúrio/farmacologia , Extratos Vegetais/farmacologia , Strychnos nux-vomica , Intoxicação Alcoólica/tratamento farmacológico , Análise de Variância , Animais , Peixes-Gato , Modelos Animais de Doenças , Eritrócitos/metabolismo , Humanos , Técnicas In Vitro , Membranas Intracelulares/efeitos dos fármacos , Permeabilidade/efeitos dos fármacos
2.
Pesqui. vet. bras ; 37(4): 368-378, Apr. 2017. tab, graf
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-895427

RESUMO

ladonia substellata Vainio is a lichen found in different regions of the world, including the Northeast of Brazil. It contains several secondary metabolites with biological activity, including usnic acid, which has exhibited a wide range of biological activities. The aim of this study was to evaluate the in vitro antimicrobial activity of the organic extract of C. substellata and purified usnic acid. Initially, Staphylococcus spp., derived from samples of skin and ears of dogs and cats with suspected pyoderma and otitis, were isolated and analyzed. In antimicrobial susceptibility testing against Staphylococcus spp., 77% (105/136) of the isolates were resistant to the antimicrobials tested. In the assessment of biofilm production, 83% (113/136) were classified as producing biofilm. In genetic characterization, 32% (44/136) were positive for blaZ, no isolate (0/136) was positive for the mecA gene, and 2% (3/136) were positive for the icaD gene. The in vitro antimicrobial activity of the organic extract of C. substellata and purified usnic acid against Staphylococcus spp. ranged from 0.25mg/mL to 0.0019mg/mL, inhibiting bacterial growth at low concentrations. The substances were more effective against biofilm-producing bacteria (0.65mg/mL-0.42mg/mL) when compared to non-biofilm producing bacteria (2.52mg/mL-2.71mg/mL). Usnic acid and the organic extract of C. substellata can be effective in the treatment of pyoderma and otitis in dogs and cats caused by Staphylococcus spp.(AU)


Cladonia substellata Vainio é um líquen encontrado em diversos continentes do mundo, inclusive no nordeste do Brasil, possui vários metabólitos secundários com atividade biológica, entre eles, o ácido úsnico, que tem apresentado uma vasta gama de atividades biológicas. O objetivo deste trabalho foi avaliar a atividade antimicrobiana in vitro do extrato orgânico de C. substellata e do ácido úsnico purificado. Para isto, foram isolados Staphylococcus spp. de amostras de pele e orelha de cães e gatos com suspeita de piodermatite e otite. No teste de sensibilidade aos antimicrobianos frente Staphylococcus spp., 77% (105/136) foram resistentes. Na avaliação da produção de biofilme 83% (113/136) foram classificadas como produtoras de biofilme. Na caracterização genotípica, 32% (44/136) foram positivos para o gene blaZ, nenhum isolado (n=136) foi positivo para o gene mecA, e 2% (3/136) foram positivos para o gene icaD. A atividade antimicrobiana in vitro do extrato orgânico de C. substellata e do ácido úsnico purificado para Staphylococcus spp. variou de 0,25mg/ml a 0,0019mg/ml, inibindo o crescimento bacteriano em baixas concentrações. Foram mais eficazes contra bactérias produtoras de biofilme (0,65mg/ml-0,42mg/ml) quando comparadas às não produtoras de biofilme (2,52mg/ml-2,71mg/ml). Viabilizando a utilização do ácido úsnico e do extrato orgânico de C. substellata, no tratamento de otite e piodermatite em cães e gatos com o envolvimento de Staphylococcus spp.(AU)


Assuntos
Animais , Gatos , Cães , Staphylococcus , Extratos Vegetais/uso terapêutico , Usnea/uso terapêutico , Anti-Infecciosos/uso terapêutico , Biofilmes , Líquens
3.
Br Homeopath J ; 90(2): 79-85, 2001 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-11341461

RESUMO

Adult toads, Bufo melanostictus, were administered Nux vomica (Nux v) 30 prepared with and without succussion on the tongue. The drug was mixed with sterile distilled water at the rate 0.05ml/ml water and given orally 0.05ml/individual. The control consisted of blank ethanol solution. Seeds of Strychnos nuxvomica were ground and extracted with 90% ethanol in the laboratory. Nux v 30 was prepared by successive dilution and succussion in 30 steps, Nux v 30 u was prepared by successive dilution only. Four hours after treatment, toads were given 25% ethanol i.p. at 8g/kg body weight. The duration of ethanol induced sleep time was recorded for each toad. Both Nux v 30 and Nux v 30 u significantly reduced ethanol induced sleep time in toads as compared to their respective controls. Electronic, infra red and nuclear magnetic resonance spectra of Nux v 30, Nux v 30 u and their diluent medium (90% ethanol) show marked differences from each other. These dilutions and ethanol 30 and ethanol 30 u show marked differences from each other with respect to spin-lattice relaxation time (T1) and chemical shift. The difference has been attributed to the variation in intra and inter-molecular association of ethanol and water.


Assuntos
Etanol/antagonistas & inibidores , Materia Medica/administração & dosagem , Materia Medica/farmacologia , Reflexo/efeitos dos fármacos , Sono/efeitos dos fármacos , Estricnina/administração & dosagem , Estricnina/farmacologia , Administração Oral , Animais , Bufonidae , Raios Infravermelhos , Espectroscopia de Ressonância Magnética
4.
Homeopathy ; 91(4): 217-20, 2002 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-12422924

RESUMO

Mercuric chloride 30c and Mercuric iodide 30c were prepared by successive dilution in 30 steps of 1:100 followed by sonication at 20KHz for 30s at each step. Both were prepared in two media: 90% ethanol and distilled water. Three preparations of Mercuric chloride 30 in water were used: 12-month old, 1-month old and 4-day old. The controls for the water and ethanol-water preparations were pure water 30c and 90% ethanol 30c, respectively. For the three water preparations there were three matched controls of water 30c of the same ages. Each potentized substance or its control was mixed with distilled water 1:100 before testing. Hydrolysis of starch by alpha-amylase was measured by the standard procedure after incubation for 15 min at 27 degrees C. Mercuric chloride 30c and Mercuric iodide 30c in both water and aqueous ethanol media, enhanced enzyme activity significantly, compared to their respective controls. Mercuric chloride 30c, prepared in water 12 months previously, produced no significant change in the enzyme activity compared to its control. We hypothesize that the structure of the active molecule imprinted on water polymers during the process of dynamization. The specifically structured water interacts with the active sites of alpha-amylase, modifying its activity. Ethanol molecules have large non-polar part stabilizing the water structure and thus retaining activity for a longer time.


Assuntos
Desinfetantes/farmacologia , Homeopatia , Iodetos/farmacologia , Cloreto de Mercúrio/farmacologia , Compostos de Mercúrio/farmacologia , alfa-Amilases/metabolismo , Etanol , Humanos , Técnicas In Vitro , Amido , Fatores de Tempo , Água , alfa-Amilases/efeitos dos fármacos
6.
Homeopathy (Londres. 2002) ; 91(4): 217-220, oct. 2002. tab
Artigo em Inglês | HomeoIndex (homeopatia) | ID: hom-6857

RESUMO

Mercuric chloride 30c and Mercuric iodide 30c were prepared by successive dilution in 30 steps of 1:100 followed by sonication at 20 KHz for 30s at each step. Both were prepared in... (AU)


Assuntos
Pesquisa Homeopática Básica , alfa-Amilases , Amido , Mercurius Corrosivus , Hidrólise
7.
Br. homoeopath. j ; 90(2): 79-85, apr. 2001. graf, tab
Artigo em Inglês | HomeoIndex (homeopatia) | ID: hom-5705

RESUMO

Adult toads, Bufo melanostictus, were administered Nux vomica (Nux v) 30 prepared with and without succussion on the tongue. The drug was mixed with sterile distilled water at the rate 0.05ml/individual. The control consisted... (AU)


Assuntos
Animais , Dinamização , /uso terapêutico , Potência , Alcoolismo/terapia
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