[Research progress on mechanism of delaying senility of Chinese materia medica in recent years].

Recently investigating mechanisms on delaying aging of traditional Chinese medicine (TCM) and discovering high effective related medicines have been become hot spot and have achieved some progress. This review comprehensively analyzed the mechanism of TCM on delaying senility in recent years. The modern researches have demonstrated that Chinese materia medica and compound formulas can retard aging process by anti-oxidant activity of free radical, modulating metabolism of neuroendocrine, balancing immunological function, prolonging telomere length and promoting telomerase activity in cells, anti-DNA damage of cells, and controlling expression of gene and protein involved in cell proliferation.

Development of vimentin and glial fibrillary acidic protein immunoreactivities in the brain of gray mullet (Chelon labrosus), an advanced teleost.

Previous studies in teleosts have revealed the presence of the intermediate filaments vimentin (Vim) and glial fibrillary acidic protein (GFAP) in glial cells of the spinal cord and/or some brain regions, but there is no comprehensive study of their distribution and developmental changes in fishes. Here, the distribution of Vim and GFAP immunoreactivities was studied in the brain of larvae, juveniles, and adults of an advanced teleost, the gray mullet (Chelon labrosus). A different sequence of appearance was observed for expression of these proteins: Vim levels decreased with age, whereas GFAP increased. In general, both immunoreactivities were expressed early in perikarya and endfeet of ependymocytes (tanycytes), whereas expression in radial processes appeared later. In large larvae, the similar expression patterns of Vim and GFAP suggest that some of these glial cells contain both proteins. Subependymal radial glia cells were observed mainly in the optic tectum, exhibiting Vim and GFAP immunoreactivity. The only immunoreactive cells with astrocyte-like morphology were observed in the optic chiasm of the adult, and they were positive for both GFAP and Vim. The perivascular processes of glial cells showed a different distribution of Vim and GFAP during development and had a caudorostral sequence of appearance of immunoreactivities similar to that observed for ependymal and radial glia cells. Several circumventricular organs (the organon vasculosum hypothalami, saccus vasculosus, and area postrema) exhibited highly specialized Vim- and/or GFAP-expressing glial cells. The glial cells of the midline septa of several brain regions were also Vim and/or GFAP immunoreactive. In the adult brain, tanycytes retain Vim expression in several brain regions. As in other vertebrates, the regions with Vim-immunoreactive ventricular and midline glia may represent areas with the capability of plasticity and regeneration in adult brain.

[Lipid peroxidation of central nerve system in aged guinea pig and antioxidation effect of the method extract from Pegasus laternarius].

OBJECTIVE: To study the lipid peroxidation products level in aged animals central nerve system and the antioxidation effect of the methol extract from Pegasus laternarius. METHODS: The lipid peroxidation product MDA was tested by spectrophotometer. RESULTS: Compared with the 10 month-old guinea pig, the MDA in 32 month-old guinea pig central nerve system obviously elevated, there were some difference in different fields of tested guinea pig brain, the level of MDA in hypothalamus increased biggest (up to 161.7%), cerebral cortex 93.7%, cerebella 84.9%, brain stem 81.2%, spinal cord 90.7%, rest of the cerebrum 58.9%. The method extract from Pegasus laternarius 10, 20, 40 mg/kg and ginseng saponin 20 mg/kg could reduce the level of MDA in tested brain field of aged animals. The method extract from Pegasus laternarius had stronger activity in brain stem, spinal cord and hypothalamus. CONCLUSION: The lipid peroxidation in aged animal may be increased, the method extract from Pegasus laternarius and ginseng saponin have a protective effect on neuron in central nerve system of aged animals from free radical hurt.

[Senility-preventing effect of erlingcankang decoction].

Experiments with Erlingcankang Decoction showed that when given to silk worms it could noticeably prolong the growth period of larvae and raise an average of 10-day survival of the male moths; when given to mice it could prolong their life; and when given to on old rats it could markedly raise the contents of SOD in the liver and red cells, lower the content of MAO-B in the brain, LF in the brain and adrenal gland and also LPO in the liver.

[Effect of Oviductus Ranae on Cyclin D1, CDK6 and P15 expressions in the liver tissue of aged male rats].

OBJECTIVE: To investigate the effect of Oviductus Ranae (OR) on the expressions of CyclinD1, CDK6 and P15 in the liver of aged male rats. METHODS: Eighteen male SD rats were randomly divided into 3 equal groups, namely the OR group, VE group and ageing model group. The rats received subcutaneous injection of D-galactose for 6 weeks to establish the aging models, and another 6 rats were injected daily with normal saline (NS) to serve as the normal control group. From the third week of the experiment, the rats were given oral OR or Vitamin E (VE) accordingly till the sixth week. After completion of the drug administration, all the rats were sacrificed for detecting the expressions of CyclinD1, CDK6 and P15 in the liver tissue by Western blotting. RESULTS: The relative expression levels of CyclinD1, CDK6 and P15 in the liver of the rats in the OR group were 41.73-/+0.54, 23.29-/+0.30 and 1.49-/+0.30, respectively, significantly up-regulated as compared with those in the ageing model group (P<0.01). The expressions of the proteins were obviously down-regulated in the model group in comparison with those in the normal control group. CONCLUSIONS: OR treatment can lower the expressions of Cyclin D1 and CDK6 in the liver to enhance the liver cell proliferation in aged male rats. OR also promotes the expression of P15 through a feedback mechanism to prevent excessive proliferation of the cells. The effect of OR against ageing is mediated possibly by up-regulation of the proteins associated with the cell proliferation in the liver, a mechanism different from that of VE.