Hypothalamic transcriptional expression of the kisspeptin system and sex steroid receptors differs among polycystic ovary syndrome rat models with different endocrine phenotypes

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Marcondes, Rodrigo Rodrigues; Carvalho, Kátia Cândido; Giannocco, Gisele; Duarte, Daniele Coelho; Garcia, Natália; Soares-Junior, José Maria; da Silva, Ismael Dale Cotrim Guerreiro; Maliqueo, Manuel; Baracat, Edmund Chada; Maciel, Gustavo Arantes Rosa.

Clinics ; 72(8): 510-514, Aug. 2017. tab, graf

Artigo em Inglês | LILACS | ID: biblio-890718

OBJECTIVES:

Polycystic ovary syndrome is a heterogeneous endocrine disorder that affects reproductive-age women. The mechanisms underlying the endocrine heterogeneity and neuroendocrinology of polycystic ovary syndrome are still unclear. In this study, we investigated the expression of the kisspeptin system and gonadotropin-releasing hormone pulse regulators in the hypothalamus as well as factors related to luteinizing hormone secretion in the pituitary of polycystic ovary syndrome rat models induced by testosterone or estradiol.

A single injection of testosterone propionate (1.25 mg) (n=10) or estradiol benzoate (0.5 mg) (n=10) was administered to female rats at 2 days of age to induce experimental polycystic ovary syndrome. Controls were injected with a vehicle (n=10). Animals were euthanized at 90-94 days of age, and the hypothalamus and pituitary gland were used for gene expression analysis.

Rats exposed to testosterone exhibited increased transcriptional expression of the androgen receptor and estrogen receptor-β and reduced expression of kisspeptin in the hypothalamus. However, rats exposed to estradiol did not show any significant changes in hormone levels relative to controls but exhibited hypothalamic downregulation of kisspeptin, tachykinin 3 and estrogen receptor-α genes and upregulation of the gene that encodes the kisspeptin receptor.

Testosterone- and estradiol-exposed rats with different endocrine phenotypes showed differential transcriptional expression of members of the kisspeptin system and sex steroid receptors in the hypothalamus. These differences might account for the different endocrine phenotypes found in testosterone- and estradiol-induced polycystic ovary syndrome rats.

Animais Feminino Hormônio Liberador de Gonadotropina/análise Hipotálamo/química Kisspeptinas/análise Hormônio Luteinizante/metabolismo Hipófise/metabolismo Síndrome do Ovário Policístico/química Modelos Animais de Doenças Regulação para Baixo Estradiol Expressão Gênica Hormônio Liberador de Gonadotropina/genética Hipotálamo/metabolismo Kisspeptinas/genética Fenótipo Síndrome do Ovário Policístico/genética Síndrome do Ovário Policístico/metabolismo Ratos Wistar Reação em Cadeia da Polimerase em Tempo Real Receptores Androgênicos/análise Receptores de Estrogênio/análise Testosterona Regulação para Cima

Biblioteca responsável: BR1.1