ABSTRACT
We compared the capability of human fibroblasts to populate porous polycaprolactone (PCL) scaffolds modified during fabrication with surface-active agents Triton Ð¥-100 (type 1 scaffold) and polyvinylpyrrolidone (type 2 scaffold). The mean fiber diameter in both scaffolds was almost the same: 3.90±2.19 and 2.46±2.15 µ, respectively. Type 1 scaffold had higher surface density and hydrophilicity, when type 2 scaffold was 1.6 times thicker. The cells were seeded on the scaffolds by the dynamic seeding technique and then cultured in Petri dishes with nutrient medium in a humid atmosphere. During 3-day culturing, no cell release from the matrix was noted. DAPI staining proved the presence of cells in both scaffolds. However, in type 1 scaffold the cells populated the whole thickness, while in type 2 scaffold, the cells were present only in the superficial layer. These findings suggest that PCL scaffolds modified with Triton Ð¥-100 or polyvinylpyrrolidone are not cytotoxic, but the structure of the scaffold treated with Triton Ð¥-100 is more favorable for population with cells.
Subject(s)
Cell Proliferation/drug effects , Fibroblasts/drug effects , Octoxynol/pharmacology , Polyesters/pharmacology , Povidone/pharmacology , Tissue Scaffolds , Biocompatible Materials , Electrochemical Techniques , Fibroblasts/cytology , Humans , Hydrophobic and Hydrophilic Interactions , Octoxynol/chemistry , Polyesters/chemistry , Porosity , Povidone/chemistry , Primary Cell Culture , Skin/cytology , Skin/drug effects , Surface-Active Agents/chemistry , Surface-Active Agents/pharmacology , Tissue EngineeringABSTRACT
BACKGROUND: Twenty two patients with congestive cardiac failure treated surgically by dynamic cardiomyoplasty (CMP) with m. latissimus dorsi were examined. Myocardial perfusion was assessed with (199)TlCl scintigraphy combined with dipyridamole stress-test. In order to obtain direct evidence of myocardial perfusion from muscular flap we also injected a bolus of (99m)Tc into a. thoracodorsalis, with simultaneous blood sampling from coronary sinus. Haemodynamic parameters were assessed using radionuclide angiography. METHODS: In a year of follow-up all the patients were assigned to one of two groups: eleven patients demonstrated improvement in clinical status (first group) and in another group comprising eleven persons no positive effect or deterioration were obvious (second group). The patients of the first group before operation revealed two times less persistent defect size than patients of the second group. Analysis of integral index of persistent defect revealed more expressive differences between groups. Before the surgical treatment the patients with improvement in clinical status after cardiomyoplasty demonstrated greater size of reversible defect in comparison with patients of the second group. In the second group coronary fraction of thallium accumulation was 1.4 times higher in comparison to the first group, as the result of myocardial hypertrophy in patients with bad prognosis. There were no significant differences between the two groups in Il/m level before cardiomyoplasty. Before the surgical treatment the patients with improvement in clinical status after cardiomyoplasty demonstrated greater ejection fraction in comparison with patients of the second group. RESULTS: Cardiomyoplasty led to a decrease in the mean size of reversible defects due to indirect revascularisation. This hypothesis was testified to by the fact that in patients after cardiomyoplasty nuclide appeared in coronary sinus at 10-12th seconds after injection into artery thoracodorsalis through anastomoses between the latissimus dorsi muscle and the myocardium. The time of appearance of the second wave of rise gamma-counting in blood samples from coronary sinus reflects the repeated entry of radiopharmaceutical in myocardium after recirculation.
ABSTRACT
The pulmonary haemodynamics and thyroid hormone level were investigated in 21 patients with EKS-222 (VVI) pacemaker, implanted after successful transvenous electrical endocardial His-bundle fulguration (TEF). Triiodthyronine (T3) and thyroxine (T4) were determined by radioimmunoassay, pulmonary circulation parameters were estimated by means of radiocardiography and radionuclide ventriculography using an Omega 500 gamma-camera and a "Scintron MX" computer system. Patients with atrial fibrillation (AF) during VVI pacing had higher level of T3 and T4 and better pulmonary circulation parameters than those with persistent sinus node activity during paced rhythm.
Subject(s)
Atrial Fibrillation/physiopathology , Cardiac Pacing, Artificial , Pulmonary Circulation , Thyroid Hormones/blood , Adolescent , Adult , Atrial Fibrillation/blood , Atrial Fibrillation/etiology , Cardiac Pacing, Artificial/adverse effects , Female , Heart Block/complications , Humans , Male , Middle Aged , Tachycardia, Paroxysmal/complications , Tachycardia, Paroxysmal/therapy , Tachycardia, Supraventricular/complications , Tachycardia, Supraventricular/therapyABSTRACT
Thirty-five cardiomyoplasty procedures were performed in five patients with dilated and 30 patients with ischemic cardiomyopathies. Russian-made cardiomyostimulators (CMS) were implanted in these patients. Twenty-one patients underwent a one-step procedure using a left thoracotomy for cardiomyoplasty, while in 14 patients, a two-step procedure that included a mid-line sternotomy was carried out. During the average follow-up period of 9 +/- 2.1 months, studies were carried out that showed in survivors improved clinical functional status and decreased heart failure symptoms. Evidence of revascularization of the ischemic myocardium from the latissimus dorsi muscle (LDM) wrap was obtained by angiography and radioactive scintigraphy. In five patients, the CMS had to be removed due to complications, and in all these patients, their clinical functional status deteriorated following the cessation of cardiomyostimulation.