[PROCUREMENT OF SANITARY AND EPIDEMIOLOGICAL WELFARE IN THE REGION OF-CITY-RESORT SOCHI FOR DANGEROUS AND NATURAL-FOCI INFECTIOUS DIS- EASES IN 2015].

AIM: Analysis ofresults of epidemiologic monitoring especially dangerous, natural-foci and other infectious diseases, asill as epizootologic activity of natural foci of infection on,the terri- tory, of city-resort Sochi. MATERIALS AND METHODS: Laboratory studies of 820. samples by PCR; im- mune- and bacteriologic methods were carried out, among those 344 - clinical material, 12 - water from open bodies and 321 - field material. Molecular-genetic identification of-143 strains of Vibrio-cholerae, isolated from open water bodies of city-resort Sochi, was-carried out,. RESULTS: Circulation of causative agents of Q fever, tularemia and hemorrhagic fever with renal syndrome of Dobrava-Adler genotypes was established, -as well as rickettsia of tick spotted fever group Predomination of rotaviruses (70.9%) was detected during study of samples of clinical material in etiologic structure of sporadically emerging acute intestine infections. Relatively high temperature values of river water during summer months (from 18, to 30 C), exit of sulfide mineral waters into the riverbed, that create-alkaline medium, seasonal reduction of river debit and speed of water current facilitated sustenance of contamination of water of Agura river by V.cholerae. CONCLUSION: Activity of natural foci of the infectious disease and contamination of Agura iver by V.cholerae Ο1, (atoxigenic) gives evidence on the necessity of constant control of epidemiologic situation by dangerous and natural-foci infectious diseases on the territory of city-resort Sochi, as well as study regional features, of their causative agents, including using genetic methods.

[Analysis of foreign experience of maintenance of biological safety of the Olympic Games].

The analysis of the international experience in providing measures of health and disease safety at the Olympic Games was done. The stages of the formation of bio-security system at public events were considered, including measures to prevent infection outbreaks, the use of computer and information technologies.

[Improvement of laboratory diagnostics of cholera due to genetically altered (hybrid) variants of cholera Vibrio biovar El Tor].

AIM: Improvement of laboratory diagnostics of cholera taking into the account appearance of hybrid variants of cholera vibrio El Tor biovar in the 1990s. MATERIALS AND METHODS: Phenotypic and molecular-genetic properties of typical toxigenic (151 strains) and hybrid (102 strains) variants of El Tor biovar cholera vibrios, isolated in the Caucuses in 1970-1990 and 1993-1998, respectively, were studied. Toxigenicity gene DNA fragments, inherent to El Tor biovars or classic, were detected by using a reagent kit "Genes of Vibrio cholerae variant ctxB-rstR-rstC, REF" developed by us. RESULTS: Reagent kit "Genes of V. cholerae variant ctxB-rstR-rstC, REF" is proposed to be used for laboratory diagnostics of cholera during study of material from humans or environmental objects and for identification of V. cholerae 01 on genome level in PCR-analysis as a necessary addition to the classic scheme of bacteriological analysis. CONCLUSION: Laboratory diagnostics of cholera due to genetically altered (hybrid) variants of cholera vibrio El Tor biovar is based on a complex study of material from humans and environmental objects by routine bacteriologic and PCR-analysis methods with the aim of detection of gene DNA fragments in the studied material, that determine biovar (classic or El Tor), identification of V. cholerae O1 strains with differentiation of El Tor vibrios into typical and altered, as well as determination of enterotoxin, produced by the specific cholera vibrio strain (by the presence ctxB(El) or ctxB(Cl) gene DNA fragment, coding biosynthesis of CT-2 or CT-1, respectively).

[Antibacterial susceptibility/resistance of Vibrio cholerae eltor clinical strains isolated in the Caucasus during the seventh cholera pandemic].

The data on antibacterial susceptibility and resistance of Vibrio cholerae eltor phenotypes with different sets of the susceptibility or resistance markers conditioning the outbreaks and sporadic cases of cholera in the Caucasus within 1970-1998 are presented. An increase of the number of the Vibrio cholerae phenotypes resistant to tetracycline and chloramphenicol usually used in the treatment of cholera was recorded in 1990-1994 vs. 1970-1989. The El Tor cholera vibrios stored on synthetic media lost some of their resistance markers, therefore the retrospective investigation of the antibioticograms was only of approximate prognostic value in the choice of the drugs for the etiotropic treatment of cholera in view of possible outbreak of the disease.

[Organizational provision for the sanitary and epidemiological safety of the population in the regions of the Stavropol Territory affected by the flood]. / Organizatsionnoe obespechenie sanitarno-épidemiologicheskogo blagopoluchiia naseleniia v raionakh Stavrpol'skogo Kraia, postradavshikh ot navodneniia.

Materials on the organizational provision and financing of measures taken with a view to ensure the sanitary and epidemiological safety in the Stavropol Territory in connection with the high flood of 2002 are presented. Due to the joint efforts of sanitary, antiepidemic and medico-prophylactic services of the Territory, administrative organs and institutions, as well as other relevant departments, not only high quality restoration works were carried out and completed in a short time, medical assistance was given to the victims and the appearance of mass infectious diseases prevented, but also the growth social tensions in the affected areas was averted.

[Situation of brucellosis in the regions of Stavropol Territory affected by the flood of 2002]. / Obstanovka po brutsellezu v raionakh Stavropol'skogo Kraia, postradavshikh ot navodeniia v 2002 g..

The emergency situation caused by inundations and high floods on the rivers in the affected regions exerts no direct influence on brucellosis morbidity among humans. Still the urgent evacuation of agricultural animals in connection with the natural calamity, their displacement and regrouping give grounds to prognosticate the deterioration of the epizootic situation in this infection in a number of regions of the territory where no sufficient veterinary surveillance has been ensured.

[Epidemiological features of leptospirosis in Stavropol' region]. / Epidemiologicheskie osobennosti leptospiroza v Stavropol'skom krae.

The data on leptospirosis morbidity among agricultural animals and humans in the Stavropol Territory for the last 10 years, as well as the serological picture of the causative agent of leptospirosis, are presented. The epidemiological importance of the main risk factors and their influence on the development and manifestations of the epidemic process are substantiated. As revealed in this investigation, the professional character of leptospirosis morbidity among humans has been almost completely lost under modern conditions, which requires further study with a view to the development of the rational complex of prophylactic measures.

[Epizootic significance of white-toothed shrews in the natural focus of steppe type tularemia in the Stavropol' region]. / Epizooticheskoe znachenie zemleroek belozubok v prirodnom ochage tuliaremii stepnogo tipa v Stavropol'skom krae.

The observations indicate that anthropogenic activities cause structural changes with time among tularemia pathogen carriers in the microfocus. The epizootic and epidemic values of animal species, such as dwarf hamsters and house mice, decrease while those of insect-eating animals, namely white-toothed shrews that refer to Group II increase, in terms of tularemia infection susceptibility. In white-toothed shrews, tularemia infection rates are 4-6-times greater than those in other small mammals that refer to Group I.