Advanced digital image analysis method dedicated to the characterization of the morphology of filamentous fungus.

Filamentous fungi have a complex morphology that induces fermentation process development issues, as a consequence of viscosity increase and diffusion limitations. In order to better understand the relationship between viscosity changes and fungus morphology during fermentations of Trichoderma reesei, an accurate image analysis method has been developed to provide quantitative and representative data for morphological analysis. This method consisted of a new algorithm called FACE that allowed sharp images to be created at all positions, segmentation of fungus, and morphological analysis using skeleton and topological approaches. It was applied and validated by characterizing samples of an industrial strain of Trichoderma reesei that had or had not been exposed to an extreme shear stress. This method allowed many morphological characteristics to be identified, among which nine relevant criteria were extracted, regarding the impact of shear stress on the fungus and on the viscosity of the fermentation medium.

[Dr. Samuel Serge Voronoff (1866-1951) or "the quest for eternal youth"]. / Le Docteur Samuel Serge Voronoff (1866-1951) ou "la quête de l'éternelle jeunesse".

Samuel Serge Voronoff, a French physician and surgeon of Russian origin was the Khédive's personal physician from 1896 to 1910 and the instigator of modern medicine in Egypt. He was later a student and friend of Alexis Carrel as soon as 1910 and directed a service of bone grafts during World War I. Between 1912 and 1949 he published the results of his experimental work at his Voronoff Foundation of the Collège de France and at Grimaldi where he performed homografts of endocrine glands of cattle and corresponding heterografts between great primates and man. Contested since 1922 by his colleagues for his results however histologically confirmed and improved durable, Voronoff who had an audience in the Académie des Sciences will proceed his research with success during the period preceding World War II. He grafted old people in senior homes and Government cattle in Algeria, training followers in Italy and California. In 1939 he gave all his research facilities at the Collège de France to René Leriche (1879-1955) and remained on the American continent until 1945. At that time his theories became obsolete in view of the progress in endocrinology and his laboratories were destroyed during the war. He died in Lausanne in 1951 at 85. The recent epidemics caused by HIV suggests to study the work he performed in the Collège de France.