ABSTRACT
Three new HLA class I alleles were described in the Spanish population. HLA-A*68:169 and -B*39:129 show one amino acid replacement at the α1-domain, compared to A*68:02 (P47 > L47) and -B*39:06 (S11 > A11), respectively. HLA-B*07:298 presents one nucleotide mutation within exon 1, resulting in a new amino acid position -14, L>Q, which has not been previously described in any HLA protein. Prediction of the B*07:298 signal peptide cleavage did not show significant differences in comparison with that obtained for the rest of HLA-B genes.
Subject(s)
Alleles , Base Sequence , HLA-A Antigens/genetics , HLA-B Antigens/genetics , HLA-B7 Antigen/genetics , Sequence Analysis, DNA , Amino Acid Sequence , HLA-A Antigens/chemistry , HLA-B Antigens/chemistry , HLA-B7 Antigen/chemistry , Haplotypes , Humans , Peptides/chemistryABSTRACT
A novel HLA-DRB1*13:216 allele was characterized in a Spanish bone marrow donor.
Subject(s)
Alleles , Amino Acid Substitution , Exons , HLA-DRB1 Chains/genetics , Mutation , Base Sequence , Bone Marrow Transplantation , Gene Expression , HLA-DRB1 Chains/immunology , Histocompatibility Testing , Humans , Sequence Analysis, DNA , Spain , Tissue DonorsABSTRACT
Three new HLA class I alleles, HLA-A*02:620, HLA-B*27:150 and HLA-B*07:05:01:02, were described in the Spanish Caucasoid population.
Subject(s)
Alleles , HLA-A Antigens/genetics , HLA-B Antigens/genetics , Genes, MHC Class I/immunology , HLA-A Antigens/immunology , HLA-B Antigens/immunology , Humans , Spain , White People/geneticsABSTRACT
Three novel HLA class II alleles, DRB1*08:70, DQA1*01:13 and DQA1*03:01:03, were characterized.
Subject(s)
Alleles , Histocompatibility Antigens Class II/genetics , Amino Acid Sequence , Exons , HLA-DQ alpha-Chains/chemistry , HLA-DQ alpha-Chains/genetics , HLA-DRB1 Chains/chemistry , HLA-DRB1 Chains/genetics , Histocompatibility Antigens Class II/chemistry , Humans , Lymphoma, Non-Hodgkin/genetics , Sequence Analysis, DNAABSTRACT
HLA-B*18:105 shows two nucleotide differences regarding B*18:22 (97 AGC>AGG, 99 TAC>TAT) and B*18:52 (94 ACC>ATC, 95 CTC>ATC).
Subject(s)
Alleles , HLA-B Antigens/genetics , Hispanic or Latino/genetics , White People/genetics , Base Sequence , Exons/genetics , Humans , Molecular Sequence Data , Sequence AlignmentABSTRACT
HLA-A*31:01:02:02 differs from A*31:01:02 in a single nucleotide mutation at intron 3, nucleotide position 1000 (G > A).
Subject(s)
Alleles , HLA-A Antigens/genetics , Polymorphism, Single Nucleotide , Tissue Donors , Base Sequence , Bone Marrow Transplantation , Codon , Exons , Gene Expression , HLA-A Antigens/immunology , Histocompatibility Testing , Humans , Introns , Molecular Sequence Data , Sequence AlignmentABSTRACT
HLA-B*49:34 shows one nucleotide difference regarding B*49:01:01 at codon 66 (ATC>GTC, I66>V66).
Subject(s)
Alleles , HLA-B Antigens/genetics , Point Mutation , Amino Acid Sequence , Amino Acid Substitution , Blood Donors , Exons , Genotype , HLA-B Antigens/immunology , Histocompatibility Testing , Humans , Molecular Sequence Data , Platelet Transfusion , Polymerase Chain Reaction , Sequence Alignment , Sequence Analysis, DNA , Spain , White PeopleABSTRACT
Two new HLA-A null alleles were characterized, A*11:210N and A*26:107N.
Subject(s)
Genes, MHC Class I , HLA-A Antigens/genetics , Adult , Alleles , Amino Acid Sequence , Base Sequence , Humans , Molecular Sequence Data , Sequence Alignment , Sequence Homology, Amino Acid , Sequence Homology, Nucleic Acid , Spain , Tissue DonorsABSTRACT
HLA-B*44:203 shows one nucleotide difference to B*44:03:01 at codon 171 (TAC>CAC, Y171>H171).
Subject(s)
Alleles , Amino Acid Substitution , HLA-B Antigens/genetics , Polymorphism, Single Nucleotide , Base Sequence , Codon , Exons , Fetal Blood/chemistry , Fetal Blood/immunology , HLA-B Antigens/classification , HLA-B Antigens/immunology , Histocompatibility Testing , Humans , Molecular Sequence Data , Protein Isoforms/classification , Protein Isoforms/genetics , Protein Isoforms/immunology , Sequence Alignment , SpainABSTRACT
HLA-DRB1*11:153 differs from DRB1*11:131 by one nucleotide at position 286 where C > A, (codon 67 CTC>ATC), resulting in an amino acid substitution, 67L>67I.
Subject(s)
Alleles , Databases, Nucleic Acid , HLA-DRB1 Chains/genetics , Base Sequence , Humans , Molecular Sequence DataABSTRACT
HLA-A*03:168N differs from HLA-A*03:01:01 by a single nucleotide substitution resulting in a coding change Y27X.
Subject(s)
Alleles , HLA-A Antigens/genetics , Hematopoietic Stem Cells/metabolism , Histocompatibility Testing , Sequence Analysis, DNA , Tissue Donors , Base Sequence , Exons/genetics , Humans , Male , Molecular Sequence Data , Norway , Sequence Alignment , SerotypingABSTRACT
HLA-B*08:108 shows one nucleotide difference regarding B*08:01:01 at codon 109 (CTC>TTC, L109>F109).
Subject(s)
Alleles , Exons , HLA-B8 Antigen/genetics , Polymorphism, Single Nucleotide , Base Sequence , Bone Marrow Transplantation , Codon , HLA-B8 Antigen/immunology , Histocompatibility Testing , Humans , Molecular Sequence Data , Sequence Alignment , Sequence Analysis, DNA , Tissue Donors , White PeopleABSTRACT
HLA-B*49:24 shows one nucleotide difference regarding B*49:10 at codon 12 (ATG>GTG). DRB1*03:64 differs from DRB1*03:01:01 in one amino acid residue at position 59, E>Q.
Subject(s)
Alleles , HLA-B Antigens/genetics , HLA-DRB1 Chains/genetics , Sequence Analysis, DNA , Amino Acid Sequence , HLA-B Antigens/chemistry , HLA-DRB1 Chains/chemistry , Humans , Molecular Sequence Data , Protein Structure, TertiaryABSTRACT
The new HLA-DPB1*142:01 allele differs from DPB1*26:01:02 and DPB1*56:01 at codons 65 (I65>L65) and 35 (F35>Y35), respectively.
Subject(s)
Alleles , Graft Rejection , HLA-DP beta-Chains/genetics , Kidney Transplantation , Polymorphism, Single Nucleotide , Base Sequence , Codon , Exons , Fatal Outcome , Histocompatibility Testing , Humans , Molecular Sequence Data , Peru , Sequence Alignment , Sequence Analysis, DNA , Tissue DonorsABSTRACT
HLA-B*51:153 shows two nucleotide differences compared with B*51:08 at codon 163 (CTG>ACG).
Subject(s)
Alleles , HLA-B Antigens/genetics , Point Mutation , Base Sequence , Exons , HLA-B Antigens/immunology , Haplotypes , Histocompatibility Testing , Humans , Molecular Sequence Data , Polymerase Chain Reaction , Sequence Alignment , Sequence Analysis, DNA , SpainABSTRACT
Two novels alleles, HLA-A*01:128 and HLA-C*05:88 are characterized.
Subject(s)
Alleles , HLA-A Antigens/genetics , HLA-C Antigens/genetics , Amino Acid Sequence , Amino Acid Substitution , Bone Marrow Transplantation , Codon , HLA-A Antigens/immunology , HLA-C Antigens/immunology , Histocompatibility Testing , Humans , Molecular Sequence Data , Mutation , Sequence Alignment , Sequence Analysis, DNA , Spain , Tissue DonorsABSTRACT
B*08:79 is composed by partial B*08:01:01 and B*07:06 sequences because of a possible recombination event within intron 2.
Subject(s)
Alleles , Exons/genetics , Genome, Human/genetics , HLA-B7 Antigen/genetics , HLA-B8 Antigen/genetics , Recombination, Genetic/genetics , Base Sequence , Humans , Molecular Sequence DataABSTRACT
To determine the complete sequence of a newly identified human leukocyte antigen (HLA)-C allele, we designed a method where the full genomic sequence of HLA-C*04 was amplified in isolation from the patient second HLA-C allele in a single polymerase chain reaction (PCR), using primers spanning its 5'- and 3'-untranslated regions. The new allele, officially designated HLA-C*04:71, differs from HLA-C*04:01:01:01 by two single-nucleotide polymorphisms: one determines substitution of phenylalanine for serine 9 at the B pocket of the peptide-binding site; the second substitution is a new polymorphism in intron 5. Phe-9 is characteristic of Cw1 alleles and its presence in C*04:71 most likely affects its peptide-binding repertoire. The principle we have used for C*04:71 isolation could be adapted for unambiguous sequence-based HLA-C typing of selected samples in a clinical setting.
Subject(s)
Alleles , HLA-C Antigens/genetics , Peptides/chemistry , Amino Acid Motifs , Base Sequence , Crystallography, X-Ray , Female , Genomics , HLA-C Antigens/chemistry , Humans , Molecular Sequence Data , Protein Binding , Sequence AlignmentABSTRACT
A*33:49 has one nucleotide change regarding A*33:01:01 at exon 3, producing an amino acid replacement at codon 97, M97 to I97.