Association between peroxisome proliferator-activated receptor-alpha, -delta and -gamma gene (PPARA, PPARD, PPARG) polymorphisms and overweight parameters in physically active men.

Peroxisome proliferator-activated receptors (PPARs) have unique functions in energy metabolism regulation but are also involved in regulation of the inflammatory process and obesity. The aim of this study was to analyse potential associations between polymorphisms of PPARA (rs1800206), PPARD (rs1053049; rs2267668) and PPARG (rs1801282) and overweight parameters. One hundred and sixty-six males, unrelated Caucasian military professionals, were recruited in the genetic case-control study conducted in the period 2016-2019. All the participants were aged 21-41 and had similar levels of physical activity. Body mass, height and body composition were measured. The participants were divided into two groups depending on their BMI (body mass index) and FMI (fat mass index). The control group consisted of people with BMI between 20.0 and 25.0 or FMI values ≤ 6, while the overweight group consisted of people with BMI of ≥ 25.0 or FMI values > 6. Genomic DNA was isolated from extracted buccal cells. All samples were genotyped using real-time polymerase chain reaction (real-time PCR). It was found that two polymorphisms rs2267668 and rs1053049 of the PPARD gene were significantly associated with BMI: SNP rs2267668 for the dominant (OR = 2.04, 95%CI 1.01-4.11, p-value = 0.04) model (A/G-G/G vs A/A). The likelihood of being overweight was over 2 times smaller for allele A. A relationship between the polymorphism of PPARG (rs1801282) and BMI was found for the overdominant (OR = 2.03, 95%CI 1.03-4.00, p-value = 0.04) model (C/G vs C/C-G/G). Significant associations were found in different models for PPARD, PPARG and PPARA genes with BMI. In SNP rs2267668 for the codominant genetic model (G/G vs A/A) (p-value = 0.04) and in SNP rs1053049 for the codominant (C/C vs T/T) (p-value = 0.01) and the recessive genetic model (C/C vs T/T-C/T) (p-value = 0.004) all polymorphisms were associated with BMI. In conclusion, it was found that three of the four polymorphisms (rs1053049, rs2267668, rs1801282) selected are associated with the risk of being overweight. Having said that, one has to bear in mind that DNA variants do not fully explain the reasons for being overweight. Therefore more research is needed to make a thorough assessment using the latest genomic methods in sequencing and genotyping, combined with epigenomics, proteomics, transcriptomics, and metabolomics.

Fat Mass Index and dietary behaviours of the Polish Border Guard officers

Background: Due to specific requirements of service, Border Guard officers should be characterized by good health. Whereas there is lack of studies assessing nutritional status as well as dietary behaviours among Polish Border Guard officers. Objective: The aim of the study was to assess the impact of dietary behaviours of the Polish Border Guard officers on the Fat Mass Index. Material and methods: The study was carried out with participation of 250 Border Guard officers (187 men and 63 women), aged 37±6; years of service: 12±6. Nutritional status was determined with the electrical bioimpedance method using the TANITA MC-780 analyzer. According to the calculated Fat Mass Index value each person was qualified to one of the following groups: fat deficit, normal fat or excess fat. The Border Guard officers were asked to fill in the original questionnaire containing questions about nutritional behaviours in line with the recommendations of the Swiss Food Pyramid for Athletes in the basic version. Results: The excessive amount of fat in every third officer of the Border Guard and numerous irregularities in eating behaviours were found. According to the food pyramid the smallest scale of rational dietary choices (especially regularity of meals, fruit and vegetable consumption) was characteristic of officers with excess fat. In addition, officers from the excess fat group obtained, on average, a lower sum of points for compliance of nutritional behaviours with the recommendations of the Swiss Food Pyramid than those from other groups (49% vs. 59% and 56%, p=0.002). Conclusions: There is a need for nutritional education and further monitoring of both the nutritional status and dietary behaviours of Border Guard officers.

Influence of Military Training and Standardized Nutrition in Military Unit on Soldiers' Nutritional Status and Physical Fitness.

Tomczak, A, Bertrandt, J, Klos, A, and Klos, K. Influence of military training and standardized nutrition in military unit on soldiers' nutritional status and physical fitness. J Strength Cond Res 30(10): 2774-2780, 2016-Despite suspension of conscription in Polish Army, trainings of soldiers are still carried out. It is expected that they will be effective and will contribute to obtaining optimum level of psychophysical efficiency that enables fulfillment of military tasks. Total of 60 soldiers took part in the study. During the 9-month military service, soldiers had 200 hours of physical training and basic military training (shooting, drill, anti-chemical training, topography, general tactics, and military equipment operation). The training lasted 8 hours everyday. To assess fitness level, 4 trials were done: long jump, pull-ups, sit-ups, and 1,000 m run. Evaluation of food was based on the analysis of full board menus using the "Tables of composition and nutritional value of food products." Energy value was assessed, and content of basic nutrients was calculated. Assessment of nutritional status was based on anthropometric measurements, such as body height, body mass, and thickness of 4 selected skinfolds. Body height and body mass were the basis for the body mass index calculation. Soldiers serving in the mechanized infantry unit, after completing the training, got better results only in 1,000 m run (from 250.3 to 233.61 seconds). During the research, an average energy value of a daily food ration planned for consumption was 4,504 kcal. This value consisted of 13.2% of energy from protein, 31.9% of energy from fat, and 54.9% from carbohydrates. In the course of military service, percentage of subjects indicating overweight increased from 10.2 to 25.4%.

The influence of vitamin B12 supplementation on the level of white blood cells and lymphocytes phenotype in rats fed a low-protein diet.

Protein malnutrition has a negative effect on body composition and some blood parameters, especially in the young growing organism. One of nutritional factors which could protect against negative consequences of protein deficiency may be B group vitamins. The aim of the study was to investigate the effect of vitamin B12 supplementation on the immune system in rats fed a standard and a low-protein diet. Rats were fed a control (20% of energy from protein) or a protein-deficient diet (4.5% of energy from protein). Half of animals in each group were additionally supplemented with vitamin B12 (300% of the daily intake). The white blood cells analysis and lymphocytes immunophenotyping (number and percentage) were performed. Low-protein diets caused disturbances in WBC and lymphocyte subpopulations in both short- (30-day) as well as long-term periods (90-day). Vitamin B12 supplementation significantly reduced the negative impact of protein malnutrition after 30 days, however had no effect on long-term malnutrition. Furthermore, vitamin B12 addition in rats fed a control diet did not affect the studied parameters. This observation opens the promise of use of vitamin B12 supplementation to improve immune system parameters in protein malnourished organisms.

Association between Diet, Physical Activity and Nutritional Status of Male Border Guard Officers.

The main factors that determine the effectiveness and reliability of duties and tasks performed by border guard officers, are very good health and maintaining a high level of psychophysical fitness that depend mainly on adequate diet and physical activity and thus, nutritional status. The aim of the study was to verify the correlations between dietary habits, physical activity level and selected nutritional status indicators. One hundred and sixty-nine male border guard officers participated in the study. A 61-item food frequency questionnaire (FFQ) was used to assess dietary habits and a long-form International Physical Activity Questionnaire (IPAQ) was used to assess physical activity. Fat mass was determined by bioelectrical impedance analysis (BIA) and bone calcification was assessed by the dual energy X-ray absorptiometry (DXA). Many correlations between dietary habits, as well as the physical activity of officers and body mass index (BMI), fat mass index (FMI) and visceral fat level (VFL) were found, while bone mineral density (BMD T-score) negatively correlated only with two food groups and 6 out of 61 products but did not correlate with physical activity. The results also confirmed many poor dietary habits and abnormalities in nutritional status. Thus, there is a need for nutritional education and further monitoring of health-related behaviors, as well as monitoring the nutritional status of border guard officers.

Assessment of the Level of Physical Activity and Body Mass Index of Soldiers of the Polish Air Force.

Level of physical activity positively affects health condition, correlates highly with level of physical fitness and contributes to the efficient performance of military tasks. The aim of the study was to assess the level of physical activity and body mass index of the Polish Air Force soldiers. A total of 543 professional soldiers (men) doing military service in military units of the Polish Air Force underwent the examination. The average age of examined soldiers amounted to 34.8 ± 9.0 years. In order to carry out the research, the long version of the International Physical Activity Questionnaire was used. Out of the total of 477 questionnaires that qualified for the analysis, a high level of physical activity was found among 81.1% of subjects, moderate 10.5% and low 8.4%. Average MET values were obtained in the following: job-related, relocation (transportation), housekeeping, recreation (leisure activities and sport). The average MET values were 4173 ± 5306 MET; 2371 ± 2725 MET; 2455 ± 4843 MET; 2421 ± 2802 MET, respectively. The average level of body mass index amounted to 25.98 ± 3.38 kg/m2. The tested Air Force soldiers were generally characterized by a high and moderate level of physical activity. Generally, there was no difference in the level of physical activity between the flight crew and the ground staff.

Assessment of the Body Composition and Bone Calcification of Students of Police Schools and Police Training Centers in Poland-A Cross-Sectional Study.

The 21st century is considered the age of malnutrition resulting in the unprecedented frequency of civilization diseases. Among these disorders, obesity is particularly distinguished and considered an epidemic-scale disease. For this reason, conducting studies on obesity and counteracting this phenomenon is essential. Research from recent years indicates a problem of excessive body weight among officers of uniformed services, who should be characterized by good health and fitness level due to the specificity of the work. As the problem of obesity affects every fourth Pole, research in uniformed services seems to be essential from health and national security perspectives. The presented study aimed to determine the elements of nutritional status in 289 students of Polish police schools and police training centers. Body composition was determined by bioelectrical impedance analysis, and bone calcification assessment was conducted by the DXA densitometric method. Based on BMI and body fat content, body weight disorders were found in 31.8% of all examined students. Densitometric test results showed changes in bone calcification of varying severity in 26.6% of the total number of respondents. The presence of obesity in students of police schools and training centers proves that the present nutrition model is energetically unbalanced, while the demonstrated disorders of bone calcification indicate an improper condition of mineral nutrition.

The Influence of FTO, FABP2, LEP, LEPR, and MC4R Genes on Obesity Parameters in Physically Active Caucasian Men.

Obesity is a complex multifactorial abnormality that has a well-confirmed genetic basis. However, the problem still lies in identifying the polymorphisms linked to body mass and composition. Therefore, this study aimed to analyze associations between FTO (rs9939609), FABP2 (rs1799883), and LEP (rs2167270), LEPR (rs1137101), and MC4R (rs17782313) polymorphisms and obesity-related parameters. Unrelated Caucasian males (n = 165) were recruited. All participants had similar physical activity levels. The participants were divided into two groups depending on their body mass index (BMI) and fat mass index (FMI). All samples were genotyped using real-time polymerase chain reaction (real-time PCR). When tested individually, only one statistically significant result was found. The FTO A/T polymorphism was significantly associated with FMI (p = 0.01). The chance of having increased FMI was >2-fold higher for the FTO A allele carriers (p < 0.01). Gene−gene interaction analyses showed the additional influence of all investigated genes on BMI and FMI. In summary, it was demonstrated that harboring the FTO A allele might be a risk factor for elevated fat mass. Additionally, this study confirmed that all five polymorphisms are involved in the development of common obesity in the studied population and the genetic risk of obesity is linked to the accumulation of numerous variants.

Assessment of Energy Expenditure of Police Officers Trained in Polish Police Schools and Police Training Centers.

Knowledge of the energy expenditure related to the training of policemen allows for assessment of the intensity of the work performed and is an indispensable element of planning and implementing nutrition. This study on energy expenditure comprised a total of 280 persons, students of two Polish police schools and two police training centers. The energy expenditure of policemen was determined based on measurements of the heart rate using Polar RC3 GPS heart rate monitors. The energy expenditure of policemen associated with the training process in the police schools and training centers ranged from 1793 to 3043 kcal/8 h and amounted to 2314 ± 945 kcal/8 h during training on average. The values of energy expenditure related to a typical training day in Polish institutions conducting police training are diverse and depend on the specificity and nature of the training. According to the criteria for assessing the burden of work, the work performed by police officers can be classified as hard work and very hard work.

Association Between Diet, Physical Activity and Body Mass Index, Fat Mass Index and Bone Mineral Density of Soldiers of the Polish Air Cavalry Units.

Research from recent years indicates a problem of excessive body weight among soldiers, who, due to the kind of work carried out, should possess good health and fitness levels. The aim of the study was to determine the association between diet and physical activity and the nutritional status of soldiers of the Polish Air Cavalry Units. One hundred and twenty male soldiers (aged 28 ± 5 years) completed a questionnaire (food frequency questionnaire, long-form International Physical Activity Questionnaire). Body composition was determined by bioelectrical impedance analysis, and bone calcification of the forearm was assessed by the DXA (Dual Energy X-ray Absorptiometry) densitometric method. This study confirmed the association between both the diet and physical activity and body mass index (BMI), fat mass index (FMI), and bone mineral density (BMD) expressed as T-score. Significant negative correlations were found between BMI and the frequency of consumption of cereal products, meat products and fish, and nonalcoholic beverages, between FMI and cereal products, and between BMD T-score and meat products and fish, fat, nuts, and grains, sweets and snacks, and nonalcoholic beverages. Physical activity expressed as metabolic equivalent (MET-minutes/week) negatively correlated with FMI (but not BMI) and positively correlated with the BMD T-score. This study confirmed numerous irregularities in eating behavior and in nutritional status indices; therefore, there is a need for nutritional education and further monitoring of both dietary behaviors and nutritional status of soldiers.

An Attempt to Utilize the Body Composition Analyzer and the Functional Movement Screen (FMS) Test to Determine Injury Risk in Soldiers.

INTRODUCTION: Injuries are the most significant health problem of military services. Military medical surveillance data are useful for determining the magnitude and causes of the injury problem, identifying possible prevention targets and monitoring trends among military personnel. Soldiers serving in the Polish Armed Forces took part in the research. The aim of the study was to identify the most common injuries of the musculoskeletal system and to analyze causes of injuries. An additional goal was to examine whether there is a relationship between selected values of body composition indexes, the result of the functional movement screen (FMS) test and occurrence of an injury. MATERIAL AND METHODS: The Polish Armed Forces soldiers serving in the land forces, military police, air forces took part in the research. The first stage was to complete the questionnaire regarding injuries in the last 5 years, their reasons, and the circumstances of their occurrence. After completing the survey, subjects were measured and weighed on the body composition analyzer. The next step was to perform a FMS to evaluate the locomotor system. RESULTS: In the groups of examined Polish soldiers, the most frequent injuries concerned lower limbs and, above all, a knee joint. The leading causes of injuries were activities resulting from the service as well as playing football and volleyball. CONCLUSIONS: Parameters from the body composition analyzer such as body mass index, muscle mass distribution on lower limbs as well as evaluation in the FMS test may be helpful for the initial assessment of predisposition to injury.

An assessment of energy expenditure of soldiers serving in the Polish chemical units, performing training tasks in 2 types of personal chemical protective clothing - comparative studies.

OBJECTIVES: Individual protection against contamination is the kind of protection provided to persons in an environment contaminated with radioactive substances, and war toxic or biological agents, including the use of gas masks, and insulating or filtration skin protection agents. The aim of this work was to perform a comparative assessment of energy expenditure of chemical troop soldiers wearing L-2 insulating personal protective clothes (PPC) and FOO-1 filter-sorptive PPC during training in the training ground conditions. Surveys on the subjective assessment of comfort related to using the L-2 and FOO-1 PPC were carried out as well. MATERIAL AND METHODS: A total of 29 men doing the same training tasks, wearing the L-2 PPC and FOO-1 filter-sorptive protective clothing with an MP-5 filter gas mask, underwent the examination. Measurements of energy expenditure values were done based on the frequency of heart contractions, recorded by the Polar Sport Tester 810 heart rate monitor. RESULTS: It was found that an average energy expenditure of soldiers, resulting from performing training tasks, was lower among the subjects wearing the FOO- 1 filter-sorptive PPC, compared to the energy load of the ones using the L-2 PPC. CONCLUSIONS: According to Christensen's classification of work severity, the average energy expenditure makes it possible to qualify works performed by soldiers using both types of PPC to moderate work. In the opinion of the examined soldiers serving in the chemical troops, the FOO-1 filter-sorptive PPC increases the sense of security while on a threatened ground. The FOO-1 filter-sorptive PPC is assessed by chemical troop soldiers as better because it hinders tasks fulfillment to a lesser extent. Int J Occup Med Environ Health. 2019;32(5):645-52.

Relationships between bone mineral density and new indices of body composition in young, sedentary men and women.

INTRODUCTION: Data concerning the relationship between body fat and BMD are equivocal since both positive and negative effects have been noted. Recently, the index of fat mass (IFM) representing subjects with different body fat and similar lean mass and index of lean mass (ILM) representing subjects with different lean body mass and similar body fat, have been used to evaluate body composition effect on BMD in middle-aged women. This study aimed at determination of ILM and IFM association with BMD in young men and women. MATERIAL AND METHODS: A total of 212 university students of Public Health (125 women and 87 men) participated in the study. Body composition was determined by the bioelectrical impedance method (BIA) using BC 418 MA equipment (Tanita Co., Japan). Fat mass and fat free mass were used to calculate ILM and IFM. Bone mineral density was measured on the wrist of the non-dominant hand using the DEXA method and EXA 3000 equipment (HFS Ltd., Korea). BMD was evaluated using Z-score, with values lower than -2.0 indicating inadequate BMD for subject chronological age. RESULTS: Exclusively in women, IFM was markedly and positively correlated with Z-score (r=0.366, P<0.001). In both genders, a significant relationship was found between ILM and Z-scores (r=0.420; p<0.001 and r=0.220; p<0.02 in men and women, respectively). Women with lower than median IFM but similar ILM, were characterized by significantly lower Z-scores vs. women with higher IFM (-1.016 vs. -0.512; p<0.001). Irrespective of gender, participants with higher ILM but similar IFM, were characterized by markedly higher Z-score vs. their counterparts with low ILM. CONCLUSIONS: The use of IFM and ILM in the present study, allowed the observation that in young adults lean body mass was associated with BMD, regardless of gender, while fat mass is significant for bone mineral density only in women.

[The assessment of nutritional status of the selected navy warship crew]. / Antropometryczna ocena stanu odzywienia zalogi wybranej jednostki plywajacej marynarki wojennej.

The aim of the work was the assessment of nutritional status of the ORP "PULASKI" frigate crew before long lasting training cruise. Crew of the warship consisting of 156 men, aged 22-42, underwent the nutritional status examination. The nutritional status was estimated based on the anthropometrical measurements. Body mass and body height of examined men was the base to calculate the Body Mass Index (BMI). Based on this index, using the Ferro-Luzzi classification, examined soldiers were classified into groups indicating overweight or obesity. It was observed that percentage of persons indicating overweight increased with the age. Overweight was found among 49% of examined aged up to 30, 54.3% of men aged up to 40 indicated overweight. Obesity was found among 13.5% of examined sailors aged up to 30 and among 10.9% sailors aged 40. Obesity found among sailors creates necessity to carry on large-scale prophylaxis operations including propagation of rational nutrition and healthy life style education.

Energy and nutritional value of diets used in patients alimentation and their assessment by patients of selected clinical department in the Military Medical Institute in Warsaw.

The aim of the work was laboratory assessment of energy and nutritional value of general and light diets used in patients of selected clinical department in the Military Medical Institute in Warsaw alimentation. Using questionnaire method the assessment of diets was done by patients too. Meals given to patients in hospital not always fulfilled nutritional requirements. Additional consumption of supplementary products did not always meet the requirements of proper nutrition. Half of examined patients appraised nutrition variety as good but at the same time claimed the there was not enough fruits and vegetables.

Influence of protein deficient diet, vitamin B[sub]2[/sub] supplementation and physical training on serum composition of polyunsaturated fatty acids (PUFAs) in rats.

[b]Introduction[/b]. Prolonged shortages of protein in the diet significantly alter the composition and content of polyunsaturated fatty acids (PUFA) in tissues and body fluids. One of nutritional factors which may reduce negative effects of protein malnutrition might be vitamin B[sub]2[/sub] due to its influence on lipids metabolism. [b]Objective. [/b]The aim of the study was to investigate the influence of low protein (LP) diet enriched with vitamin B[sub]2[/sub] on the content and composition of PUFA in the blood serum of rats treated with dosed physical exercise. [b]Materials and method. [/b]The experiment was carried out for 3 months on 72 growing male Wistar rats divided into 5 groups. Animals were fed ad libitum on a diet with an energy value of 350 kcal/100 g, in which 4.5% of the energy was provided by protein. In the control diet, 20% of the energy was provided by protein. Two groups were fed the diet enriched with vitamin B2. The two groups of tested animals were trained for 5 days a week. [b]Results.[/b] LP diet caused a decrease in α-linolenic acid (ALA) after 30 days, and a decrease in docosahexaenoic acid (DHA) after 60 days of experiment, compared with rats fed the control diet. After 60 and 90 days of the experiment, a significant decrease was noted in arachidonic acid (AA) in serum of trained rats, compared with sedentary rats fed the LP diet. Physical activity increased LA (mainly on day 30), EPA (on day 90) and reduced AA content (on day 90) in serum of rats fed the LP diet. B2 supplementation in the trained LP group did not change the EPA and AA dependence; however, there was a decrease in LA content in comparison to the non-supplemented trained group. [b]Conclusions. [/b] Results of this study suggest that all investigated factors (protein deficiency, physical exercise and supplementation of vitamin B2) have significant impact on PUFA composition of serum in rats.

Effect of 3-year education in the Main Fire Service School on the fitness level of students.

INTRODUCTION AND OBJECTIVE: The aim of the study was to determine changes in the fitness level among students of the Main Fire Service School during 3-year's education. MATERIAL AND METHODS: A total of 75 male and 5 female students took part in the study. Fitness level measurements were carried out before beginning the studies (Measurement 0) and after the 1st, 2nd and 3rd years at the school (Measurement 1, 2, 3). The following tests were performed to estimate level of physical fitness for men: pull-ups on a bar, a 50 m sprint and 1,000 m run, and for women: sit-ups, medical ball (2 kg) throw from above the head and a 4 x10 m shuttle run. RESULTS: General fitness level (average number of points from 3 attempts) of the male and female students improved. Improvement of fitness level between Measurement 0 (male students 60.3p.±7.58; female students 61.60p.±3.08), and Measurements 1, 2, 3 (men 64.59p.±6.87; 64.41p.±7.17; 64.03p.±7.65 and women 68.40p.±1.99; 66.67p.±2.36; 68.87±1.87 respectively), was found. The same relationship was found while analysing results of individual competitions. Only speed endurance (anaerobic-aerobic) assessed during Measurement 0 at 64.03p.±10.23, decreased after the 1st year at the school (Measurement 1 - 59.29p.±14.14) and remained on the same level during Measurements 2 and 3 (60.25p.±11.20 and 58.56p. ±12.80, respectively). CONCLUSIONS: Speed endurance (anaerobic-aerobic) is a capability which decreased among the students in the subsequent years. Because this capability highly correlates with activities that a fire-fighter will perform during rescue operations, it should be treated as a matter of priority during physical education classes in the Main Fire Service School.

Polyunsaturated fatty acid (PUFA) changes in serum and liver of undernourished rats given dietary vitamin B6 supplementation.

The influence of vitamin B6 on linoleic (LA), alpha-linolenic (ALA), arachidonic (AA), eicosapentaenoic (EPA) and docosahexaenoic (DHA) acid content in serum and liver of rats fed with protein-energy deficient diets for 90 d, was studied. To estimate the effect of dietary supplementation with vitamin B6 on the composition and level of fatty acids in the serum and liver of rats, two experiments were performed. In these experiments control rats were fed ad libitum semisynthetic isocaloric diets of 1,466.5 kJ/100 g (350 kcal/100 g) throughout 90 d while the examined rats were offered 50% and 30% of the previously determined daily intake of the diet consumed in the control group. The experimental diet was supplemented with vitamin B6 to the level 4-times higher than in the control diet. A reduction to the half consumption of a standard diet supplemented with vitamin B6 caused a significant decrease of LA and ALA in blood serum at 30 and 60 d. At 90 d of the experiment the value of LA was lower and the content of AA was higher in comparison to the control group. After 30 d of consumption of vitamin B6 enriched diet in rats subjected to feed restriction to only 30% of the control intake, an increase of ALA and a decrease of AA, EPA and DHA were noticed in serum. At 60 d an increase of DHA was observed. Ninety days of feeding this diet caused a significant increase of AA level. Feeding animals for 90 d with a vitamin B6 enriched diet, with limited consumption to 50%, caused a significant increase of ALA content in liver. Further limitation of this diet consumption to 30%, caused a significant decrease of LA and ALA and an increase of EPA content.

Content of polyunsaturated fatty acids (PUFAs) in serum and liver of rats fed restricted diets supplemented with vitamins B2, B6 and folic acid.

The aim of study was to investigate an influence of nutritional deficiency and dietary addition of vit. B(2), B(6) and folic acid on PUFAs content in rats' serum and liver. Limitation of consumption full value diet to 50% of its previously determined daily consumption, enriched with m/a vitamins, significant decreased of linoleic (LA) and alpha-linolenic (ALA) acids as well as distinctly increased arachidonic (AA) and docosahexaenoic (DHA) acids content in serum in 30th day. In 60th day lower content of AA and DHA fatty acids was found. Nutrition with such diet, lasting 90 days caused decrease of LA content and increase of AA. Diet limitation to its 30% of daily consumption decreased of eicosapentaenoic acid (EPA) and DHA in the 30th day, while AA and DHA content was increased in the 60th day. Distinct decrease of AA content and increase of EPA content were found in the 90th day of experiment. Use of diets, with limited consumption to 50% caused increase of LA and ALA acids content while AA and DHA acids content were significantly decreased in the liver, in 90th day. Limited consumption supplemented diet to 30% caused in liver significant decrease of LA and increase of EPA acids content.

Effect of restricted feed intake and addition of vitamins B2 and B6 and folic Acid on the liver concentration of zinc and copper in rats.

The aim of the present study was to investigate the influence of nutritional deficiency and dietary addition of vitamins (B2, B6, and folate) on hepatic concentration of zinc and copper in rats. The experiment was performed on 260 growing male Wistar rats divided into 13 groups. Animals of 11 groups were fed isocaloric diets (14.7 MJ/kg) in which the 20% of energy was derived from protein. Another two groups of rats were offered diets with 9% or 4.5% of energy originating from protein. Animals of both mentioned groups and of the control group (20% of energy from protein) were offered diets ad libitum. The other 10 groups were offered 50% and 30% of the amount consumed in the control group. Eight groups, from those 10 restricted ones, were differentiated by dietary addition of vitamins B2 and B6 and folate (300% addition). Restricted feed intake did not affect the liver zinc concentration but significantly increased the copper concentration. The addition of vitamin B6 decreased the liver Zn concentration. The highest liver Cu concentration was noted in rats offered restricted diets to only 30% of intake in the control group and high in vitamin B2 and in rats supplemented with all of studied vitamins together. It suggests that vitamin B2 had the strongest impact on liver Cu concentration in rats fed restricted diets.