Chitosan nanocomposites as a nano-bio tool in phytopathogen control.

Chitosan, an economically viable and versatile biopolymer, exhibits a wide array of advantageous physicochemical and biological properties. Chitosan nanocomposites, formed by the amalgamation of chitosan or chitosan nanoparticles with other nanoparticles or materials, have garnered extensive attention across agricultural, pharmaceutical, and biomedical domains. These nanocomposites have been rigorously investigated due to their diverse applications, notably in combatting plant pathogens. Their remarkable efficacy against phytopathogens has positioned them as a promising alternative to conventional chemical-based methods in phytopathogen control, thus exploring interest in sustainable agricultural practices with reduced reliance on chemical interventions. This review aims to highlight the anti-phytopathogenic activity of chitosan nanocomposites, emphasizing their potential in mitigating plant diseases. Additionally, it explores various synthesis methods for chitosan nanoparticles to enhance readers' understanding. Furthermore, the analysis delves into elucidating the intricate mechanisms governing the antimicrobial effectiveness of these composites against bacterial and fungal phytopathogens.

Recent advances in immuno-based methods for the detection of Ralstonia solanacearum.

Ralstonia solanacearum (RS) is a widely recognized phytopathogenic bacterium which is responsible for causing devastating losses in a wide range of economically significant crops. Timely and accurate detection of this pathogen is pivotal to implementing effective disease management strategies and preventing crop losses. This review provides a comprehensive overview of recent advances in immuno-based detection methods for RS. The review begins by introducing RS, highlighting its destructive potential and the need for point-of-care detection techniques. Subsequently, it explores traditional detection methods and their limitations, emphasizing the need for innovative approaches. The main focus of this review is on immuno-based detection methods and it discusses recent advancements in serological detection techniques. Furthermore, the review sheds light on the challenges and prospects of immuno-based detection of RS. It emphasizes the importance of developing rapid, field-deployable assays that can be used by farmers and researchers alike. In conclusion, this review provides valuable insights into the recent advances in immuno-based detection methods for RS.

Development of antibody to virulence factor flagellin and its evaluation in screening Ralstonia pseudosolanacearum.

The bacterial wilt disease caused by Ralstonia pseudosolanacearum presents a notable economic risk to a variety of crucial crops worldwide. During preliminary isolation of this phytopathogen, several colonies of other saprophytic bacteria may be mistaken with it. So, the present study aims to address this issue by proposing the application of immunogenic proteins, particularly flagellin (FliC), to enable a rapid and early identification of bacterial wilt. In this study, a novel approach is unveiled for the early detection of R. pseudosolanacearum. The study exploits the immunogenic attributes of flagellin (FliC), by generating polyclonal antibodies against recombinant FliC within model organisms-rabbits and mice. The efficacy of these antibodies is meticulously assessed through discerning techniques, including DAS-ELISA and Western blot analyses, which elucidate their remarkable specificity in identifying various R. pseudosolanacearum strains. Furthermore, the introduction of antibody-coated latex agglutinating reagents offers an additional layer of confirmation, substantiating the feasibility of establishing a laboratory-based toolkit for swift screening and unambiguous identification of the bacterial wilt pathogen. This study presents a significant stride toward enhancing early diagnostic capabilities, potentially revolutionizing agricultural practices by safeguarding crop yield and quality through proactive pathogen detection and mitigation strategies.

Molecular Insights of Plant Phytochemicals Against Diabetic Neuropathy.

Diabetes and its associated complications including diabetic neuropathy have become a menacing headache for health workers and scientists all over the world. The number of diabetic individuals has been growing exponentially every day while the entire medical fraternity feels crippled and unable to handle such an enormous and anarchical scenario. The disease also demonstrates itself in the patients in numerous ways ranging from a little discomfort to death. Diabetic neuropathy has a poor prognosis since it might go unnoticed for years after the onset of diabetes. The etiology of the disease has been linked to oxidative stress caused by increased free radical production. Hyperglycemia causes multiple metabolic pathways to be activated, as well as significant oxidative stress, which becomes the major cause of cell death, culminating in Diabetic Neuropathy. So, it is the need of the hour to find out permanent treatment for this life-threatening disease. The primary goal of this study is to emphasize the potential importance of numerous processes and pathways in the development of diabetic neuropathy as well as the possible role of plant metabolites to control the disease at a molecular level. A possible mechanism was also summarized in the study about scavenging the reactive oxygen species by a flavonoid component. The study also covered the in vivo data of various plants and some of the flavonoid compounds actively studied against Diabetic Neuropathy by inhibiting or reducing the contributing factors such as proinflammatory cytokines, ROS, RNS inhibition, and upregulating the various cellular antioxidants such as GSH, SOD, and CAT.

Multifunctional role of carbon dot-based polymer nanocomposites in biomedical applications: a review.

Carbon-based 0D materials have shown tremendous potential in the development of biomedical applications of the next generation. The astounding results are primarily motivated by their distinctive nanoarchitecture and unique properties. Integrating these properties of 0D carbon nanomaterials into various polymer systems has orchestrated exceptional potential for their use in the development of sustainable and cutting-edge biomedical applications such as biosensors, bioimaging, biomimetic implants and many more. Specifically, carbon dots (CDs) have gained much attention in the development of biomedical devices due to their optoelectronic properties and scope of band manipulation upon surface revamping. The role of CDs in reinforcing various polymeric systems has been reviewed along with discussing unifying concepts of their mechanistic aspects. The study also discussed CDs optical properties via the quantum confinement effect and band gap transition which is further useful in various biomedical application studies.

Graphene in nanomedicine: A review on nano-bio factors and antibacterial activity.

Graphene-based nanomaterials possess potent antibacterial activity and have engrossed immense interest among researchers as an active armour against pathogenic microbes. A comprehensive perception of the antibacterial activity of these nanomaterials is critical to the fabrication of highly effective antimicrobial nanomaterials, which results in highly efficient and enhanced activity. These materials owing to their antimicrobial activity are utilized as nanomedicine against various pathogenic microbes. The present article reviews the antimicrobial activity of graphene and its analogs such as graphene oxide, reduced graphene oxide as well as metal, metal oxide and polymeric composites. The review draws emphasis on the effect of various nano-bio factors on the antibacterial capability. It also provides an insight into the antibacterial properties of these materials along with a brief discussion on the discrepancies in their activities as evidenced by the scientific communities. In this way, the review is expected to shed light on future research and development in graphene-based nanomedicine.

Recent advances and mechanism of antimicrobial efficacy of graphene-based materials: a review.

Graphene-based materials have undergone substantial investigation in recent years owing to their wide array of physicochemical characteristics. Employment of these materials in the current state, where infectious illnesses caused by microbes have severely damaged human life, has found widespread application in combating fatal infectious diseases. These materials interact with the physicochemical characteristics of the microbial cell and alter or damage them. The current review is dedicated to molecular mechanisms underlying the antimicrobial property of graphene-based materials. Various physical and chemical mechanisms leading to cell membrane stress, mechanical wrapping, photo-thermal ablation as well as oxidative stress exerting antimicrobial effect have also been thoroughly discussed. Furthermore, an overview of the interactions of these materials with membrane lipids, proteins, and nucleic acids has been provided. A thorough understanding of discussed mechanisms and interactions is essential to develop extremely effective antimicrobial nanomaterial for application as an antimicrobial agent.

Chitosan nanoparticles and based composites as a biocompatible vehicle for drug delivery: A review.

The shellfish processing industry is one of the largest growing industries across the globe with a market size of around USD 62B. However, it also leads to a significant environmental issue as it produces >80,000 tons of waste shells globally. Unfortunately, the slow degradation of this waste causes it to accumulate over time, posing a serious threat to the marine environment. The key solution to this problem is to recycle this sea waste into a valuable product like chitin which is further used to produce chitosan. Chitosan is a natural biopolymeric substance obtained via N-deacetylation of the chitin. The chitosan-based nanoparticles are further useful for the fabrication of biopolymeric nanocomposites which are used in various biomedical applications specifically in drug delivery. Here, we review the recent advancements in the development of chitosan-based nanocomposites as a biocompatible carrier for drug delivery, specifically focusing on gene delivery, wound healing, microbial treatment, and anticancer drug delivery. By providing a valuable and up-to-date resource, this review illuminates the current state of research concerning chitosan's pivotal role in the biomedical domain as an efficacious drug delivery agent.