ABSTRACT
The effect of different natural and synthetic immunomodulators on the spontaneous interferon (IFN) synthesis by freshly isolated resident peritoneal cells of BALB/c, NZB and C3H mice was investigated. Actinomycetal glycolipids isolated from Curtobacterium betae, Faenia rectivirgula, Rothia dentocariosa and Saccharopolyspora hirsuta at the concentration 1-20 micrograms/ml were found to potentiate the IFN synthesis by the peritoneal cells of BALB/c mice. Similar results were obtained when dsRNA, LPS of Shigella sonnei and lipid A isolated from the LPS were used. The effect of potentiation of the physiological IFN production by the immunomodulators was observed also in the cells of C3H and NZB mice. In contrast, the inhibition of the IFN synthesis was observed when the peritoneal cells of BALB/c and NZB mice were treated with imuthiol at concentration 0.1-10 micrograms/ml. Thymomodulin (TFX-Polfa) at concentration of 1-100 micrograms/ml had no effect on the spontaneous IFN production.
Subject(s)
Adjuvants, Immunologic/pharmacology , Adjuvants, Immunologic/physiology , Interferons/metabolism , Animals , Female , Interferons/biosynthesis , Mice , Mice, Inbred BALB C , Mice, Inbred C3H , Mice, Inbred NZB , Peritoneal Cavity/cytologyABSTRACT
In three cases, two of which regarded exudative pericardium inflammation and one of lung abscess, biopsy revealed negative bacteriological results. Blood cultures resulted in culture of anaerobic Gram-negative bacteria from Propionibacterium genus. Precise chemo- geno-taxonomic studies were performed which confirmed generic identification of isolated strains. These were following: in cell wall--L-DAP, alanine, glycine, galactose, glucose, mannose; lack if mycolic acids; %GC 58-59. Basing on additional bacteriological studies it was established that two strains belong to the genus Propionibacterium acnes (type I), while third strain appeared to be Propionibacterium propionicum (before Arachnia propionica). These results confirm growing participation of relative pathogenic bacteria in opportunistic infections. It suggests necessity of blood cultures in cases subjected to prolonged therapy, especially when immunosuppression is involved.