Visualizing the cortical representation of whisker touch: voltage-sensitive dye imaging in freely moving mice.

Voltage-sensitive dye imaging resolves the spatiotemporal dynamics of supragranular subthreshold cortical activity with millisecond temporal resolution and subcolumnar spatial resolution. We used a flexible fiber optic image bundle to visualize voltage-sensitive dye dynamics in the barrel cortex of freely moving mice while simultaneously filming whisker-related behavior to generate two movies matched frame-by-frame with a temporal resolution of up to 2 ms. Sensory responses evoked by passive whisker stimulation lasted longer and spread further across the barrel cortex in awake mice compared to anesthetized mice. Passively evoked sensory responses were large during behaviorally quiet periods and small during active whisking. However, as an exploring mouse approached an object while whisking, large-amplitude, propagating cortical sensory activity was evoked by active whisker-touch. These experiments demonstrate that fiber optics can be used to image cortical sensory activity with high resolution in freely moving animals. The results demonstrate differential processing of sensory input depending upon behavior.

Initiation and propagation of neuronal coactivation in the developing hippocampus.

Correlated neuronal activity is ubiquitous in developing nervous systems, where it may introduce spatiotemporal coherence and contribute to the organization of functional circuits. In this report, we used voltage-sensitive dyes and optical imaging to examine the spatiotemporal pattern of a spontaneous network activity, giant depolarizing potentials (GDPs), in rat hippocampal slices during the first postnatal week. The propagation pattern of the GDP is closely correlated to the anatomical organization of the network. In the hilus, where mossy cells and interneurons are not organized in layers, GDPs propagate at the same velocity in all directions. In CA3 and CA1, the activation is synchronous along the axis of the pyramidal cells' dendritic tree. The velocity of wave propagation is significantly different in three hippocampal subfields: it is slowest in the hilus, faster in CA3, and fastest in CA1. The velocity of horizontal propagation (along the axis of the pyramidal layer) has a large variation from trial to trial, suggesting that the horizontal velocity is determined to some extent by dynamic network factors. Imaging revealed that each GDP event is initiated from a small focus. The location of the initiation focus differs from event to event. All together, our data suggest that GDP is a propagating excitation wave, initiated from a small site, and propagating to the whole hippocampus. The spatiotemporal patterns of the wave in CA3 and CA1 areas show better synchrony along the pyramidal cell dendritic trees and progressive activation along the axis of the pyramidal cell layer.