Ecophysiological Response on Dehydration and Temperature in Terrestrial Klebsormidium (Streptophyta) Isolated from Biological Soil Crusts in Central European Grasslands and Forests.

The green algal genus Klebsormidium (Klebsormidiophyceae, Streptophyta) is a typical member of biological soil crusts (BSCs) worldwide. Ecophysiological studies focused so far on individual strains and thus gave only limited insight on the plasticity of this genus. In the present study, 21 Klebsormidium strains (K. dissectum, K. flaccidum, K. nitens, K. subtile) from temperate BSCs in Central European grassland and forest sites were investigated. Photosynthetic performance under desiccation and temperature stress was measured under identical controlled conditions. Photosynthesis decreased during desiccation within 335-505 min. After controlled rehydration, most isolates recovered, but with large variances between single strains and species. However, all K. dissectum strains had high recovery rates (>69%). All 21 Klebsormidium isolates exhibited the capability to grow under a wide temperature range. Except one strain, all others grew at 8.5 °C and four strains were even able to grow at 6.2 °C. Twenty out of 21 Klebsormidium isolates revealed an optimum growth temperature >17 °C, indicating psychrotrophic features. Growth rates at optimal temperatures varied between strains from 0.26 to 0.77 µ day-1. Integrating phylogeny and ecophysiological traits, we found no phylogenetic signal in the traits investigated. However, multivariate statistical analysis indicated an influence of the recovery rate and growth rate. The results demonstrate a high infraspecific and interspecific physiological plasticity, and thus wide ecophysiological ability to cope with strong environmental gradients. This might be the reason why members of the genus Klebsormidium successfully colonize terrestrial habitats worldwide.

Cold Acclimation Improves the Desiccation Stress Resilience of Polar Strains of Klebsormidium (Streptophyta).

Biological soil crusts (BSCs) are complex communities of autotrophic, heterotrophic, and saprotrophic (micro)organisms. In the polar regions, these biocrust communities have essential ecological functions such as primary production, nitrogen fixation, and ecosystem engineering while coping with extreme environmental conditions (temperature, desiccation, and irradiation). The microalga Klebsormidium is commonly found in BSCs all across the globe. The ecophysiological resilience of various Klebsormidium species to desiccation and other stresses has been studied intensively. Here we present the results of transcriptomic analyses of two different Klebsormidium species, K. dissectum and K. flaccidum, isolated from Antarctic and Arctic BSCs. We performed desiccation stress experiments at two different temperatures mimicking fluctuations associated with global change. Cultures grown on agar plates were desiccated on membrane filters at 10% relative air humidity until the photosynthetic activity as reflected in the effective quantum yield of photosystem II [Y(II)] ceased. For both species, the response to dehydration was much faster at the higher temperature. At the transcriptome level both species responded more strongly to the desiccation stress at the higher temperature suggesting that adaptation to cold conditions enhanced the resilience of both algae to desiccation stress. Interestingly, the two different species responded differently to the applied desiccation stress with respect to the number as well as function of genes showing differential gene expression. The portion of differentially expressed genes shared between both taxa was surprisingly low indicating that both Klebsormidium species adapted independently to the harsh conditions of Antarctica and the Arctic, respectively. Overall, our results indicate that environmental acclimation has a great impact on gene expression and the response to desiccation stress in Klebsormidium.

Biological Soil Crusts of Arctic Svalbard-Water Availability as Potential Controlling Factor for Microalgal Biodiversity.

In the present study the biodiversity of biological soil crusts (BSCs) formed by phototrophic organisms were investigated on Arctic Svalbard (Norway). These communities exert several important ecological functions and constitute a significant part of vegetation at high latitudes. Non-diatom eukaryotic microalgal species of BSCs from 20 sampling stations around Ny-Ålesund and Longyearbyen were identified by morphology using light microscopy, and the results revealed a high species richness with 102 species in total. 67 taxa belonged to Chlorophyta (31 Chlorophyceae and 36 Trebouxiophyceae), 13 species were Streptophyta (11 Klebsormidiophyceae and two Zygnematophyceae) and 22 species were Ochrophyta (two Eustigmatophyceae and 20 Xanthophyceae). Surprisingly, Klebsormidium strains belonging to clade G (Streptophyta), which were so far described from Southern Africa, could be determined at 5 sampling stations. Furthermore, comparative analyses of Arctic and Antarctic BSCs were undertaken to outline differences in species composition. In addition, a pedological analysis of BSC samples included C, N, S, TP (total phosphorus), and pH measurements to investigate the influence of soil properties on species composition. No significant correlation with these chemical soil parameters was confirmed but the results indicated that pH might affect the BSCs. In addition, a statistically significant influence of precipitation on species composition was determined. Consequently, water availability was identified as one key driver for BSC biodiversity in Arctic regions.