ABSTRACT
We describe a method of isolation of human mesenchymal stromal cells from the umbilical cord (Wharton's jelly) and human placenta: amnion, placental villi, and trophoblast. Morphology, immunophenotypic characteristics, and differentiation potencies of isolated cells were studied. The capacity of mesenchymal stromal cells from extraembryonic tissues to osteogenic, adipogenic, and chondrogenic differentiation was demonstrated and the dynamics of this process was described. The isolated cells met the criteria for multipotent mesenchymal stem cells.
Subject(s)
Amnion/cytology , Mesenchymal Stem Cells/cytology , Multipotent Stem Cells/cytology , Umbilical Cord/cytology , Wharton Jelly/cytology , Adipocytes/cytology , Adipocytes/immunology , Amnion/immunology , Antigens, CD/immunology , Antigens, CD/metabolism , Biomarkers/metabolism , Cell Differentiation , Cell Separation , Cells, Cultured , Chondrocytes/cytology , Chondrocytes/immunology , Chorionic Villi/immunology , Female , HLA-DR Antigens/immunology , HLA-DR Antigens/metabolism , Humans , Immunophenotyping , Mesenchymal Stem Cells/immunology , Multipotent Stem Cells/immunology , Osteocytes/cytology , Osteocytes/immunology , Pregnancy , Trophoblasts/cytology , Trophoblasts/immunology , Umbilical Cord/immunology , Wharton Jelly/immunologyABSTRACT
Cytogenetic analysis of 13 mesenchymal stem cell cultures isolated from normal human adipose tissue was carried out at different stages of culturing. The incidence of chromosomes 6, 8, 11, and X aneuploidy and polyploidy was studied by fluorescent in situ hybridization. During the early passages, monosomal cells were more often detected than trisomal ones. A clone with chromosome 6 monosomy was detected in three cultures during late passages.
Subject(s)
Adipose Tissue/cytology , Aneuploidy , Clone Cells , Stem Cells/cytology , Cells, Cultured , Chromosomes, Human , HumansABSTRACT
The incidence of autosomes 6 and 8 aneuploidy in stem cell cultures derived from adipose tissue was evaluated at different stages of culturing. Monosomy was more incident than trisomy during the early passages. Distribution of cultures by the incidence of aneuploidy in different chromosomes was virtually the same. Clones with chromosome 6 monosomy were detected in two cultures during late passages.