ABSTRACT
Spermatogenesis is a process in which differentiated cells are produced and the adult stem cell population-known as spermatogonial stem cells (SSCs)-is continuously replenished. However, the molecular mechanisms underlying these processes are not fully understood in the canine species. We addressed this in this study by analysing the expression of specific markers in spermatogonia of seminiferous tubules of canine testes. SSCs at different stages of reproductive development (prepubertal and adult) were examined by immunohistochemistry and flow cytometry. Glial cell-derived neurotrophic factor family receptor alpha-1 (GFRA1), deleted in azoospermia-like (DAZL) and promyelocytic leukaemia zinc finger (PLZF) were expressed in SSCs, while stimulated by retinoic acid gene 8 (STRA8) was detected only in undifferentiated spermatogonia in prepubertal testis and differentiated spermatogonia and spermatocytes in adult canine. Octamer-binding transcription factor 4 (OCT4) showed an expression pattern, and the levels did not differ between the groups examined. However, C-kit expression varied as a function of reproductive developmental stage. Our results demonstrate that these proteins play critical roles in the self-renewal and differentiation of SSCs and can serve as markers to identify canine spermatogonia at specific stages of development.
Subject(s)
Dogs/physiology , Proteins/analysis , Spermatogenesis/physiology , Spermatogonia/chemistry , Adult Germline Stem Cells/chemistry , Animals , Biomarkers/analysis , Deleted in Azoospermia 1 Protein , Flow Cytometry/veterinary , Glial Cell Line-Derived Neurotrophic Factor Receptors/analysis , Immunohistochemistry/veterinary , Kruppel-Like Transcription Factors/analysis , Male , RNA-Binding Proteins/analysis , Seminiferous Tubules/cytology , Sexual Maturation , Spermatogonia/growth & developmentABSTRACT
Chronic kidney disease (CKD) is a common clinical condition in domestic cats, characterized by tubulointerstitial, vascular and glomerular inflammation and severe fibrosis. Studies in rodent model of induced CKD have shown a decrease and stabilization of the clinical condition. In this study was evaluated the safety and effect of intrarenal and intravenous infusion of allogeneic mesenchymal stem cells (AMSCs) derived from feline amniotic membrane in cats with naturally occurring CKD. Cat AMSCs were harvested after mechanical and enzymatic digestion of amnion. A healthy cat received intrarenal injection of AMSCs guided by ultrasound in both kidneys (5 × 105 cells/kidney). Nine cats with CDK received repeated intravenous infusions of AMSCs (2 × 106 cells × 2 treatments). The clinical parameters of healthy cat did not change, but sedation and general anaesthesia was required. The number of interventions stressed the animal, and he developed transient haematuria after AMSC injection. Cats with CDK registered a significant improvement of renal function (decrease in serum creatinine and urine protein concentrations and increase in urine specific gravity). The kidney architecture and morphology did not change following the treatment. The feline AMSCs have a renoprotective effect and improve renal function in cats with naturally occurring CKD, stabilizing the clinical condition and disease progression. Thus, intravenous injection of AMSCs may be an important tool to provide welfare in cats with chronic kidney disease.
Subject(s)
Amnion/cytology , Cat Diseases/surgery , Mesenchymal Stem Cell Transplantation/veterinary , Renal Insufficiency, Chronic/veterinary , Animals , Cat Diseases/pathology , Cat Diseases/prevention & control , Cats , Female , Infusions, Intravenous/veterinary , Injections/veterinary , Kidney/pathology , Male , Mesenchymal Stem Cell Transplantation/methods , Mesenchymal Stem Cells , Renal Insufficiency, Chronic/prevention & control , Renal Insufficiency, Chronic/surgeryABSTRACT
The biosafety of innovative procedures that utilize stem cells in regenerative medicine has been addressed in several studies. Previous work has showed no tumour formation following the use of feline and human amniotic membrane-derived stem cells (AMSCs). In contrast, tumour formation was observed when canine AMSCs were utilized. These findings suggested that feline and human, but not canine, AMSCs are suitable for cell transplantation trials. This study aimed to further evaluate the feasibility of utilizing canine AMSCs for transplantation purposes as well as for felines. We tested teratoma formation following cell injection into BALB/c nude mice and then assessed expression of haematopoietic, mesenchymal, tumorigenic, pluripotency and cellular regulation markers using flow cytometry and qPCR. The use of canine AMSCs did not result in macroscopic tumour formation as determined 60 days after transplantation. The immunophenotypic characterization by flow cytometry revealed expression of mesenchymal markers (CD73 and CD90) and expression of the pluripotent marker OCT4 and SOX2. Quantitative PCR analysis revealed that there were no differences in the patterns of gene expression (CD34, CD73, OCT4, CD30 and P53) between canine and feline AMSCs, with the exception of the expression of SOX2 and CD90.
Subject(s)
Amnion/cytology , Mesenchymal Stem Cell Transplantation/adverse effects , Teratogens/analysis , Teratoma/pathology , Animals , Biomarkers , Cats , Cell Differentiation/physiology , Cell Proliferation/physiology , Cells, Cultured , Dogs , Flow Cytometry , Gene Expression , Mesenchymal Stem Cells/pathology , Mice , Mice, NudeABSTRACT
Distemper disease is an infectious disease reported in several species of domestic and wild carnivores. The high mortality rate of animals infected with canine distemper virus (CDV) treated with currently available therapies has driven the study of new efficacious treatments. Mesenchymal stem cell (MSC)-based therapy is a promising therapeutic option for many degenerative, hereditary, and inflammatory diseases. Therefore, the aim of this study was to characterize stem cells derived from the canine fetal olfactory epithelium and to assess the systemic response of animals infected with CDV to symptomatic therapy and treatment with MSCs. Eight domestic mongrel dogs (N = 8) were divided into two groups: support group (SG) (N = 5) and support group + cell therapy (SGCT) (N = 3), which were monitored over 15 days. Blood samples were collected on days 0, 6, 9, 12, and 15 to assess blood count and serum biochemistry (urea, creatinine, alanine transferase, alkaline phosphatase, gamma-glutamyl transferase, total protein, albumin, and globulin), and urine samples were obtained on days 0 and 15 for urinary evaluation (urine I). The results showed a high mortality rate (SG = 4 and SGCT = 2), providing inadequate data on the clinical course of CDV infection. MSC therapy resulted in no significant improvement when administered during the acute phase of canine distemper disease, and a prevalence of animals with high mortality rate was found in both groups due to the severity of symptoms.
Subject(s)
Antibodies, Viral/blood , Distemper/therapy , Mesenchymal Stem Cell Transplantation , Animals , Distemper/blood , Distemper/mortality , Distemper/virology , Distemper Virus, Canine/pathogenicity , Dogs , Mesenchymal Stem Cells/metabolismABSTRACT
The precise determination of the embryonic chronology is very important in reproductive biotechnologies, especially in estimating embryonic age. Thus, there is a need for greater knowledge and standardization for determining the chronology of embryonic development and functional morphology. We describe aspects of embryonic development in two domestic carnivores to add knowledge about organ peculiarities and for application in veterinary practice, in prenatal development and in the biotechnology fields. We found that the development of differential characteristics of embryonic organs occurs in the first trimester of pregnancy for both species. Thus, using the combination of the crown-rump length, macroscopic analysis and optical microscopy, it is possible to predict gestational age more precisely in animals that lack a defined breed and establish an embryonic pattern.
Subject(s)
Cats/embryology , Dogs/embryology , Embryonic Development , Organogenesis , Animals , Crown-Rump Length , Female , Gestational Age , Pregnancy , Species SpecificityABSTRACT
Very few carnivore's embryology is reported mainly restricted to old literature without new technique analyses. Also, their development focuses on pharyngeal arches and stem cell sources and the high capacity for differentiation from those cells to generate embryonic tissue. We aimed to use immunohistochemistry to prove the potentiality of these stem cell niches. The results were to highlight the timetable for the development of dogs and cats, the proper formation of pharyngeal arches and the description of these cells on first and second arches since 17-25 days of pregnancy. After that, the differentiation process is reduced.
Subject(s)
Branchial Region/embryology , Cats/embryology , Dogs/embryology , Gene Expression Regulation, Developmental/physiology , Octamer Transcription Factor-3/metabolism , Animals , Branchial Region/metabolism , Female , Octamer Transcription Factor-3/genetics , PregnancyABSTRACT
In vitro activity of caspofungin and voriconazole against 184 clinical isolates of Candida and other medically important yeasts in comparison with that of fluconazole, ketoconazole, itraconazole and amphotericin B was determined by using a disk diffusion method (Neo-Sensitabs) standardized according to the recommendations of the CLSI documents M44-A and M44-S1 (same medium: Mueller-Hinton plus methylene blue; inoculum and minimal inhibitory concentration/zone breakpoints). Seventy-two percent of clinical isolates were susceptible to caspofungin, 23.6% showed an intermediate susceptibility (most of them were Candida parapsilosis) and 4.3% were resistant (values for Candida spp. were 71.2, 23.8 and 5%, respectively). For voriconazole, 96.7% of clinical isolates were susceptible and 3.3% were resistant (Candida spp.: 96 and 3.8%, respectively). Both caspofungin and voriconazole showed high activity against a wide variety of clinically important yeasts.
Subject(s)
Antifungal Agents/pharmacology , Candida/drug effects , Drug Resistance, Fungal , Echinocandins/pharmacology , Pyrimidines/pharmacology , Triazoles/pharmacology , Amphotericin B/pharmacology , Caspofungin , Cryptococcus/drug effects , Disk Diffusion Antimicrobial Tests , Fluconazole/pharmacology , Itraconazole/pharmacology , Ketoconazole/pharmacology , Lipopeptides , Rhodotorula/drug effects , Trichosporon/drug effects , VoriconazoleABSTRACT
We have compared a commercially available tablet diffusion method for the in vitro antifungal susceptibility testing of fluconazole (FCZ) and voriconazole (VCZ) with the disk diffusion method M44 (CLSI) with 282 clinical yeast isolates. The superior stability of antifungal agents in tablets can explain the differences for each category of susceptibility by both methods.Neo-Sensitabs tablets antifungal susceptibility testing showed an excellent correlation (0.98 for FCZ and 0.98 for VCZ at 24h and 0.96 for FCZ and 0.94 for VCZ at 48 h ), a reduced percentage of disagreements (4.6% and 8.2% for FCZ at 24h and 48 h respectively; 1.1% and 2.1% for VCZ at 24h and 48 h respectively) and the absence of statistically significant difference in comparison with the reference protocol for performing antifungal susceptibility testing with the agar diffusion method.
Subject(s)
Antifungal Agents/pharmacology , Drug Resistance, Fungal/drug effects , Fluconazole/pharmacology , Microbial Sensitivity Tests/methods , Pyrimidines/pharmacology , Triazoles/pharmacology , Candida/drug effects , Cells, Cultured , Humans , In Vitro Techniques , Linear Models , Reproducibility of Results , Saccharomyces/drug effects , VoriconazoleSubject(s)
Acinetobacter baumannii/drug effects , Anti-Bacterial Agents/pharmacology , Drug Resistance, Multiple, Bacterial , Minocycline/analogs & derivatives , Piperazines/pharmacology , Humans , Hydrogen-Ion Concentration , Microbial Sensitivity Tests/methods , Minocycline/pharmacology , TigecyclineABSTRACT
1. The absorption, tissue distribution and excretion of antimony dextran glycoside (RL-712) has been studied in normal rodents.2. Some organs in the body, especially liver and spleen, retain large amounts of antimony for considerable periods of time. Excretion of antimony in the urine was low and only about 10-12% of the dose administered was excreted within the first 48 hours.3. Blood levels were maintained for at least 3 days after a single intramuscular dose to rabbits, corresponding to 14 mg Sb/kg body weight.4. Toxicity studies and tests on foetal toxicity in mice and rats, respectively, showed no abnormalities.5. The possible value of RL-712 in the prophylaxis and treatment of leishmaniasis is discussed.
Subject(s)
Antimony , Dextrans , Abnormalities, Drug-Induced , Animals , Antimony/analysis , Antimony/blood , Antimony/metabolism , Antimony/therapeutic use , Antimony/toxicity , Antimony/urine , Fetus/drug effects , Glycosides , Kidney/analysis , Leishmaniasis/drug therapy , Leishmaniasis/immunology , Liver/analysis , Liver/metabolism , Mice , Myocardium/analysis , Rabbits , Rats , Spleen/analysis , Spleen/metabolismABSTRACT
Thirty-one Norwegian clinical isolates of rapidly growing mycobacteria classified as Runyon's group IV, including 20 Mycobacterium fortuitum and 11 Mycobacterium chelonae strains, were found resistant to a majority of tuberculostatic agents. Minimal inhibitory concentration (MIC) was determined for twelve other antimicrobial agents: amikacin, tobramycin, streptomycin, cefoxitin, imipenem, norfloxacin, ciprofloxacin, doxycycline, erythromycin, fusidic acid, co-trimoxazole and capreomycin. The agar plate dilution method was employed and compared with the agar tablet diffusion method. Regression lines were established correlating MIC values and inhibition zones. The agar tablet diffusion method was found to be a simple and useful method for testing antimicrobial susceptibilities of M. fortuitum and M. chelonae, and a good correlation between MIC values and zone sizes with twelve antimicrobial agents was revealed. Correlation coefficients for most of these antimicrobial agents were around -0.90. M. chelonae was generally more resistant than M. fortuitum. Four antimicrobial agents, capreomycin, norfloxacin, ciprofloxacin and amikacin, showed differences between M. fortuitum and M. chelonae large enough to allow the zone diameter to be used diagnostically.
Subject(s)
Anti-Bacterial Agents/pharmacology , Microbial Sensitivity Tests/methods , Mycobacterium chelonae/drug effects , Mycobacterium/drug effects , Diffusion , Humans , Mycobacterium/growth & development , Mycobacterium/isolation & purification , Mycobacterium chelonae/growth & development , Mycobacterium chelonae/isolation & purification , Norway , Regression AnalysisABSTRACT
A diffusion method for determining the sensitivity of pathogenic fungi to therapeutic agents is described using tablets containing the following antibiotics: amphotericin B, clotrimazole, econazole, fluorocytosine, and miconazole. The composition of the media used, standardisation of inocula, incubation time, and temperature are detailed.
Subject(s)
Antifungal Agents/pharmacology , Fungi/drug effects , Culture Media , Diffusion , Microbial Sensitivity Tests/methods , TabletsABSTRACT
The agar diffusion method Neo-Sensitabs for sensitivity testing, was evaluated with 33 reference strains by fourteen laboratories. Tablets with 5-fluorocytosine, amphotericin B, nystatin, fluconazole, itraconazole, ketoconazole and tioconazole were used on Shadomy modified medium. These tests classify each strain as susceptible, intermediate or resistant to all tested antifungals by measuring the inhibition zone diameters. Intra and interlaboratory reproducibility was studied. Neo-Sensitabs sensitivity for fungi was easy to perform and reliable method with a reproducibility of 97.1% and superior to other commercialized methods, being specially interesting for antifungal susceptibility in vitro testing of triazole derivatives fluconazole and itraconazole.
ABSTRACT
Twelve Spanish laboratories collected 325 yeast clinical isolates during a 30 day's period, among them 224 Candida albicans, 30 Candida glabrata, and 27 Candida parapsilosis. In vitro antifungal susceptibility to amphotericin B, ketoconazole, fluconazole and itraconazole was determined by an agar diffusion test (Neo-Sensitabs, Rosco, Denmark). All the isolates tested were susceptible in vitroto amphotericin B and nearly all (97.2%) to itraconazole. In vitrosusceptibility to fluconazole and ketoconazole was high (90.2% and 91.4% of isolates, respectively) but showed variations depending on the species tested. Resistance to fluconazole and ketoconazole was low in C. albicans (4% and 3%, respectively), but 30% of Candida guilliermondii and 36% of C. glabrata isolates were resistant to fluconazole. Ketoconazole resistance was observed in 40% of C. glabrata, and 17% of Candida tropicalis. Resistance to antifungal drugs is very low in Spain and it is related to non-C. albicans isolates.
ABSTRACT
The in vitro antifungal activity of amphotericin B was compared with that of griseofulvin, ketoconazole, clotrimazole and terbinafine in 193 clinical isolates of dermatophytes and Scopulariopsis brevicaulis. An agar diffusion method was used (NeoSensitabs) to categorize the susceptibility of the isolates as susceptible, intermediate or resistant to the antifungal agents. Using this method and following a standardized protocol adapted to the growth conditions of the dermatophytes and the opportunistic mold S. brevicaulis (inoculum size, temperature and time period of incubation), it was found that the in vitro susceptibility rates were 72%, 94.3%, 81.9%, 72% and 86% for amphotericin B, terbinafine, griseofulvin, ketoconazole and clotrimazole, respectively. Resistance percentages were 12.4%, 3.6%, 18.1%, 10.4% and 4.1% for the same antifungal agents. Amphotericin B showed no antifungal activity against S. brevicaulis; its activity against dermatophytes was similar to that of ketoconazole, and lower than that for clotrimazole and terbinafine.
Subject(s)
Amphotericin B/pharmacology , Antifungal Agents/pharmacology , Arthrodermataceae/drug effects , Ascomycota/drug effects , Microbial Sensitivity TestsABSTRACT
A standardised antimicrobial sensitivity testing method for veterinary use is described. The method makes use of Neo-Sensitabs tablets, and is based in a quantitative technique relating, diameter of inhibition zones to MIC values, and to serum concentrations for the different antimicrobials used in veterinary medicine. The sensitivity testing method has been standardised with 4 different media: Danish Blood Agar, Mueller-Hinton Agar, DST Agar, and Iso-sensitest Agar and with two different inocula (according to ICS and Kirby-Bauer respectively). Information is given on cross-resistance between different antimicrobials, and basic sets of drugs are recommended for routine use. Interpretation tablets are given for both inocula (semi-confluent or confluent colonies), and a quality control procedure using two standard strains (E. Coli ATCC 25922 and Staph, aureus ATCC 25923), enables the regular control of the media and inocula used in the test.
Subject(s)
Anti-Bacterial Agents/pharmacology , Bacterial Infections/veterinary , Microbial Sensitivity Tests/standards , Animals , Cattle , Culture Media , Immunodiffusion/methods , SwineABSTRACT
Recently, some Aerococcus-like organisms (ALOs), isolated from urine and blood of elderly patients with urinary tract infection, have been described. In this study ALOs and related taxons were tested for susceptibility by agar diffusion and agar dilution methods to 15 selected antimicrobial agents for diagnostic and taxonomic considerations. ALOs were susceptible to a wide range of antimicrobials including beta-lactams, but resistant to aminoglycosides, sulphonamides, trimethoprim and nalidixic acid. By using tablets containing vancomycin, furazolidone and bacitracin, it was possible to separate ALOs from related taxons. Clustering based on antibiotic susceptibilities showed that there is little similarity between Aerococcus viridans and ALOs.