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Selection of Pseudomonas aeruginosa reference genes for RT-qPCR analysis from sputum of cystic fibrosis patients.
Costaglioli, Patricia; Barthe, Christophe; Fayon, Michael; Christoflour, Naik; Bui, Stéphanie; Derlich, Laurence; Domblides, Philippe; Crouzet, Marc; Vilain, Sébastien; Garbay, Bertrand.
Mol Cell Probes ; 28(1): 10-2, 2014 Feb.
Article in English | MEDLINE | ID: mdl-24075879

ABSTRACT

The prerequisite to monitor gene expression is the selection of reference genes for normalization of RT-qPCR results. Using 13 sputum samples collected from 9 CF patients, we demonstrated that PA2875 and PA3340 are better reference genes than the previously used clpX and oprL genes.


Subject(s)
Cystic Fibrosis/microbiology , Genes, Bacterial , Pseudomonas aeruginosa/genetics , Sputum/microbiology , Gene Expression Profiling/methods , Humans , Real-Time Polymerase Chain Reaction
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