Subject(s)
Factor V/genetics , Point Mutation , Prothrombin/genetics , Arabs/genetics , Female , Gene Frequency , Humans , Male , Morocco , Risk Factors , Thrombophilia/genetics , Venous Thrombosis/geneticsABSTRACT
OBJECTIVE: To study the chromosomal content of spermatozoa that could be selected for intracytoplasmic sperm injection (ICSI) in cases of macrocephalic sperm head syndrome. DESIGN: Case report. SETTING: Obstetrics, gynecology, urology, and reproductive biology departments. PATIENT(S): Two infertile patient candidates for ICSI presenting with total teratozoospermia (100%) with mainly large-headed spermatozoa (91% and 82%, respectively). INTERVENTION(S): Fluorescence in situ hybridization with X, Y, 18 centromeric probes on unselected spermatozoa (all migrated spermatozoa) and specifically on selected spermatozoa with normal-sized heads. MAIN OUTCOME MEASURE(S): Percentage of polyploid, diploid, aneuploid, and normal haploid spermatozoa, according to X, Y, 18 chromosome centromeric probes on selected spermatozoa (head size compatible with ICSI). RESULT(S): All the nonselected spermatozoa were abnormal, diploid, or polyploid. The rate of normal ploidy (haploid cells) among the selected sperm population was 1 per 28 for patient 1 and 5 per 51 for patient 2. CONCLUSION(S): This very low proportion of normal haploid spermatozoa among selected spermatozoa contraindicated ICSI for the two patients. We suggest performance of this selection and analysis before including (or not), in an ICSI program, patients with macrocephalic sperm head syndrome, associated or not with preimplantation genetic diagnosis.
Subject(s)
Chromosome Aberrations , Infertility, Male/genetics , Infertility, Male/pathology , Ploidies , Sperm Head/pathology , Spermatozoa/abnormalities , Chromosomes, Human, Pair 18 , Chromosomes, Human, X , Chromosomes, Human, Y , Contraindications , Diploidy , Humans , In Situ Hybridization, Fluorescence , Male , Oligospermia/genetics , Oligospermia/pathology , Polyploidy , Sperm Injections, IntracytoplasmicABSTRACT
OBJECTIVE: To evaluate the influence of aging on testicular histology and the aneuploidy rate in testis postmeiotic cells. DESIGN: Comparison between older men and younger men. SETTING: Deceased donors and patients who requested assisted reproductive technology (ART). PATIENT(S): Thirty-six older men (61-102 years old) and 10 younger men (29-40 years old). INTERVENTION(S): Testes were obtained from 35 deceased donors, and testicular biopsies were obtained from 11 patients who requested ART. MAIN OUTCOME MEASURE(S): Histomorphometry of testis and fluorescent in situ hybridization (FISH), with a three-probe set X, Y, and 18. RESULT(S): The histomorphometric study showed a thickening of the basal membrane when spermatogenesis was arrested. The number of germinal and Sertoli cells decreased as age increased. The rate of aneuploidy of postmeiotic cells was 1.1% for the control group, 1.29% for older subjects with preserved spermatogenesis, and 14.28% for the subjects with an arrested spermiogenesis. Only this last figure was higher than the control group. CONCLUSION(S): The rate of aneuploidy in older subjects (61-95 years old) with preserved spermatogenesis was not statistically different from that found in the control group; it was increased in older subjects with arrested spermatogenesis.