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1.
Chembiochem ; 25(15): e202400187, 2024 Aug 01.
Article in English | MEDLINE | ID: mdl-38639212

ABSTRACT

Understanding the mechanisms of drug action in malarial parasites is crucial for the development of new drugs to combat infection and to counteract drug resistance. Proteomics is a widely used approach to study host-pathogen systems and to identify drug protein targets. Plasmodione is an antiplasmodial early-lead drug exerting potent activities against young asexual and sexual blood stages in vitro with low toxicity to host cells. To elucidate its molecular mechanisms, an affinity-based protein profiling (AfBPP) approach was applied to yeast and P. falciparum proteomes. New (pro-) AfBPP probes based on the 3-benz(o)yl-6-fluoro-menadione scaffold were synthesized. With optimized conditions of both photoaffinity labeling and click reaction steps, the AfBPP protocol was then applied to a yeast proteome, yielding 11 putative drug-protein targets. Among these, we found four proteins associated with oxidoreductase activities, the hypothesized type of targets for plasmodione and its metabolites, and other proteins associated with the mitochondria. In Plasmodium parasites, the MS analysis revealed 44 potential plasmodione targets that need to be validated in further studies. Finally, the localization of a 3-benzyl-6-fluoromenadione AfBPP probe was studied in the subcellular structures of the parasite at the trophozoite stage.


Subject(s)
Antimalarials , Plasmodium falciparum , Proteomics , Vitamin K 3 , Antimalarials/pharmacology , Antimalarials/chemistry , Plasmodium falciparum/drug effects , Vitamin K 3/pharmacology , Vitamin K 3/chemistry , Vitamin K 3/metabolism , Protozoan Proteins/metabolism , Photoaffinity Labels/chemistry , Photoaffinity Labels/pharmacology , Saccharomyces cerevisiae/metabolism , Saccharomyces cerevisiae/drug effects , Molecular Probes/chemistry , Molecular Probes/pharmacology , Proteome/analysis , Proteome/metabolism , Molecular Structure
2.
J Org Chem ; 89(4): 2104-2126, 2024 02 16.
Article in English | MEDLINE | ID: mdl-37267444

ABSTRACT

This work describes the reactivity and properties of fluorinated derivatives (F-PD and F-PDO) of plasmodione (PD) and its metabolite, the plasmodione oxide (PDO). Introduction of a fluorine atom on the 2-methyl group markedly alters the redox properties of the 1,4-naphthoquinone electrophore, making the compound highly oxidizing and particularly photoreactive. A fruitful set of analytical methods (electrochemistry, absorption and emission spectrophotometry, and HRMS-ESI) have been used to highlight the products resulting from UV photoirradiation in the absence or presence of selected nucleophiles. With F-PDO and in the absence of nucleophile, photoreduction generates a highly reactive ortho-quinone methide (o-QM) capable of leading to the formation of a homodimer. In the presence of thiol nucleophiles such as ß-mercaptoethanol, which was used as a model, o-QMs are continuously regenerated in sequential photoredox reactions generating mono- or disulfanylation products as well as various unreported sulfanyl products. Besides, these photoreduced adducts derived from F-PDO are characterized by a bright yellowish emission due to an excited-state intramolecular proton transfer (ESIPT) process between the dihydronapthoquinone and benzoyl units. In order to evidence the possibility of an intramolecular coupling of the o-QM intermediate, a synthetic route to the corresponding anthrones is described. Tautomerization of the targeted anthrones occurs and affords highly fluorescent stable hydroxyl-anthraquinones. Although probable to explain the intense visible fluorescence emission also observed in tobacco BY-2 cells used as a cellular model, these coupling products have never been observed during the photochemical reactions performed in this study. Our data suggest that the observed ESIPT-induced fluorescence most likely corresponds to the generation of alkylated products through reduction species, as demonstrated with the ß-mercaptoethanol model. In conclusion, F-PDO thus acts as a novel (pro)-fluorescent probe for monitoring redox processes and protein alkylation in living cells.


Subject(s)
Indolequinones , Vitamin K 3/analogs & derivatives , Mercaptoethanol , Indolequinones/chemistry , Anthraquinones
3.
Molecules ; 29(7)2024 Apr 04.
Article in English | MEDLINE | ID: mdl-38611899

ABSTRACT

2,6-Diaryl-4H-tetrahydro-thiopyran-4-ones and corresponding sulfoxide and sulfone derivatives were designed to lower the major toxicity of their parent anti-kinetoplatidal diarylideneacetones through a prodrug effect. Novel diastereoselective methodologies were developed and generalized from diarylideneacetones and 2,6-diaryl-4H-tetrahydro-thiopyran-4-ones to allow the introduction of a wide substitution profile and to prepare the related S-oxides. The in vitro biological activity and selectivity of diarylideneacetones, 2,6-diaryl-4H-tetrahydro-thiopyran-4-ones, and their S-sulfoxide and sulfone metabolites were evaluated against Trypanosoma brucei brucei, Trypanosoma cruzi, and various Leishmania species in comparison with their cytotoxicity against human fibroblasts hMRC-5. The data revealed that the sulfides, sulfoxides, and sulfones, in which the Michael acceptor sites are temporarily masked, are less toxic against mammal cells while the anti-trypanosomal potency was maintained against T. b. brucei, T. cruzi, L. infantum, and L. donovani, thus confirming the validity of the prodrug strategy. The mechanism of action is proposed to be due to the involvement of diarylideneacetones in cascades of redox reactions involving the trypanothione system. After Michael addition of the dithiol to the double bonds, resulting in an elongated polymer, the latter-upon S-oxidation, followed by syn-eliminations-fragments, under continuous release of reactive oxygen species and sulfenic/sulfonic species, causing the death of the trypanosomal parasites in the micromolar or submicromolar range with high selectivity indexes.


Subject(s)
Chagas Disease , Prodrugs , Pyrans , Safrole/analogs & derivatives , Sulfhydryl Compounds , Humans , Animals , Oxides , Oxidation-Reduction , Mammals
4.
J Org Chem ; 86(15): 10055-10066, 2021 08 06.
Article in English | MEDLINE | ID: mdl-34264092

ABSTRACT

3-Benzylmenadiones were obtained in good yield by using a blue-light-induced photoredox process in the presence of Fe(III), oxygen, and γ-terpinene acting as a hydrogen-atom transfer agent. This methodology is compatible with a wide variety of diversely substituted 1,4-naphthoquinones as well as various cheap, readily available benzyl bromides with excellent functional group tolerance. The benzylation mechanism was investigated and supports a three-step radical cascade with the key involvement of the photogenerated superoxide anion radical.


Subject(s)
Ferric Compounds , Quinones , Catalysis , Hydrogen , Oxidation-Reduction
5.
Chemistry ; 26(15): 3314-3325, 2020 Mar 12.
Article in English | MEDLINE | ID: mdl-31746499

ABSTRACT

A series of highly diversified 3-aroylmenadiones was prepared by a new Friedel-Crafts acylation variant/oxidative demethylation strategy. A mild and versatile acylation was performed between 1,4-dimethoxy-2-methylnaphthalene and various activated/deactivated benzoic and heteroaromatic carboxylic acids, in the presence of mixed trifluoroacetic anhydride and triflic acid, at room temperature and in air. The 1,4-dimethoxy-2-methylnaphthalene-derived benzophenones were isolated in high yield, and submitted to oxidative demethylation with cerium ammonium nitrate to produce 3-benzoylmenadiones. All 1,4-naphthoquinone derivatives were investigated as redox-active electrophores by cyclic voltammetry. The electrochemical data recorded for 3-acylated menadiones are characterized by a second redox process, the potentials of which cover a wide range of values (500 mV). These data emphasize the ability of the generated structural diversity at the 3-aroyl chain of these electrophores to fine-tune their corresponding redox potentials. These properties are of significance in the context of antimalarial drug development and understanding of the mechanism of bioactivation/action.

6.
Molecules ; 25(21)2020 Nov 01.
Article in English | MEDLINE | ID: mdl-33139647

ABSTRACT

Neglected parasitic diseases remain a major public health issue worldwide, especially in tropical and subtropical areas. Human parasite diversity is very large, ranging from protozoa to worms. In most cases, more effective and new drugs are urgently needed. Previous studies indicated that the gold(I) drug auranofin (Ridaura®) is effective against several parasites. Among new gold(I) complexes, the phosphole-containing gold(I) complex {1-phenyl-2,5-di(2-pyridyl)phosphole}AuCl (abbreviated as GoPI) is an irreversible inhibitor of both purified human glutathione and thioredoxin reductases. GoPI-sugar is a novel 1-thio-ß-d-glucopyranose 2,3,4,6-tetraacetato-S-derivative that is a chimera of the structures of GoPI and auranofin, designed to improve stability and bioavailability of GoPI. These metal-ligand complexes are of particular interest because of their combined abilities to irreversibly target the essential dithiol/selenol catalytic pair of selenium-dependent thioredoxin reductase activity, and to kill cells from breast and brain tumors. In this work, screening of various parasites-protozoans, trematodes, and nematodes-was undertaken to determine the in vitro killing activity of GoPI-sugar compared to auranofin. GoPI-sugar was found to efficiently kill intramacrophagic Leishmania donovani amastigotes and adult filarial and trematode worms.


Subject(s)
Anthelmintics , Antineoplastic Agents , Antiprotozoal Agents , Auranofin , Coordination Complexes , Gold , Helminthiasis/drug therapy , Neoplasms/drug therapy , Protozoan Infections/drug therapy , Animals , Anthelmintics/chemistry , Anthelmintics/pharmacology , Antineoplastic Agents/chemistry , Antineoplastic Agents/pharmacology , Antiprotozoal Agents/chemistry , Antiprotozoal Agents/pharmacology , Auranofin/chemistry , Auranofin/pharmacology , Cattle , Cell Line, Tumor , Coordination Complexes/chemistry , Coordination Complexes/pharmacology , Drug Evaluation , Gold/chemistry , Gold/pharmacology , Helminthiasis/metabolism , Helminthiasis/pathology , Humans , Neoplasms/metabolism , Neoplasms/pathology , Protozoan Infections/metabolism , Protozoan Infections/pathology
7.
Molecules ; 24(24)2019 Dec 10.
Article in English | MEDLINE | ID: mdl-31835450

ABSTRACT

With the aim to clarify the mechanism(s) of action of nitroaromatic compounds against the malaria parasite Plasmodium falciparum, we examined the single-electron reduction by P. falciparum ferredoxin:NADP+ oxidoreductase (PfFNR) of a series of nitrofurans and nitrobenzenes (n = 23), and their ability to inhibit P. falciparum glutathione reductase (PfGR). The reactivity of nitroaromatics in PfFNR-catalyzed reactions increased with their single-electron reduction midpoint potential (E17). Nitroaromatic compounds acted as non- or uncompetitive inhibitors towards PfGR with respect to NADPH and glutathione substrates. Using multiparameter regression analysis, we found that the in vitro activity of these compounds against P. falciparum strain FcB1 increased with their E17 values, octanol/water distribution coefficients at pH 7.0 (log D), and their activity as PfGR inhibitors. Our data demonstrate that both factors, the ease of reductive activation and the inhibition of PfGR, are important in the antiplasmodial in vitro activity of nitroaromatics. To the best of our knowledge, this is the first quantitative demonstration of this kind of relationship. No correlation between antiplasmodial activity and ability to inhibit human erythrocyte GR was detected in tested nitroaromatics. Our data suggest that the efficacy of prooxidant antiparasitic agents may be achieved through their combined action, namely inhibition of antioxidant NADPH:disulfide reductases, and the rapid reduction by single-electron transferring dehydrogenases-electrontransferases.


Subject(s)
Antimalarials/chemistry , Antimalarials/pharmacology , Glutathione Reductase/antagonists & inhibitors , Oxidation-Reduction/drug effects , Plasmodium falciparum/drug effects , Plasmodium falciparum/enzymology , Antioxidants/chemistry , Antioxidants/pharmacology , Dose-Response Relationship, Drug , Enzyme Inhibitors/chemistry , Enzyme Inhibitors/pharmacology , Erythrocytes/drug effects , Erythrocytes/metabolism , Erythrocytes/parasitology , Ferredoxin-NADP Reductase/metabolism , Humans , Inhibitory Concentration 50 , Molecular Structure , NADP/metabolism
8.
Mol Microbiol ; 104(2): 306-318, 2017 04.
Article in English | MEDLINE | ID: mdl-28118506

ABSTRACT

The goal to prevent Plasmodium falciparum transmission from humans to mosquitoes requires the identification of targetable metabolic processes in the mature (stage V) gametocytes, the sexual stages circulating in the bloodstream. This task is complicated by the apparently low metabolism of these cells, which renders them refractory to most antimalarial inhibitors and constrains the development of specific and sensitive cell-based assays. Here, we identify and functionally characterize the regulatory regions of the P. falciparum gene PF3D7_1234700, encoding a CPW-WPC protein and named here Upregulated in Late Gametocytes (ULG8), which we have leveraged to express reporter genes in mature male and female gametocytes. Using transgenic parasites containing a pfULG8-luciferase cassette, we investigated the susceptibility of stage V gametocytes to compounds specifically affecting redox metabolism. Our results reveal a high sensitivity of mature gametocytes to the glutathione reductase inhibitor and redox cycler drug methylene blue (MB). Using isobologram analysis, we find that a concomitant inhibition of the parasite enzyme glucose-6-phosphate dehydrogenase-6-phosphogluconolactonase, a key component of NADPH synthesis, potently synergizes MB activity. These data suggest that redox metabolism and detoxification activity play an unsuspected yet vital role in stage V gametocytes, rendering these cells exquisitely sensitive to decreases in NADPH concentration.


Subject(s)
Plasmodium falciparum/drug effects , Antimalarials/pharmacology , Carboxylic Ester Hydrolases/metabolism , Carboxylic Ester Hydrolases/physiology , Gene Expression Regulation , Genes, Reporter , Glucosephosphate Dehydrogenase/metabolism , Glucosephosphate Dehydrogenase/physiology , Luciferases , Multienzyme Complexes/metabolism , Multienzyme Complexes/physiology , Oxidation-Reduction/drug effects , Plasmodium falciparum/genetics , Plasmodium falciparum/metabolism , Plasmodium falciparum/physiology
9.
Org Biomol Chem ; 16(15): 2647-2665, 2018 04 18.
Article in English | MEDLINE | ID: mdl-29542786

ABSTRACT

Malaria is a tropical parasitic disease threatening populations in tropical and sub-tropical areas. Resistance to antimalarial drugs has spread all over the world in the past 50 years, thus new drugs are urgently needed. Plasmodione (benzylmenadione series) has been identified as a potent antimalarial early lead drug, acting through a redox bioactivation on asexual and young sexual blood stages. To investigate its metabolism, a series of plasmodione-based tools, including a fully 13C-labelled lead drug and putative metabolites, have been designed and synthesized for drug metabolism investigation. Furthermore, with the help of UHPLC-MS/MS, two of the drug metabolites have been identified from urine of drug-treated mice.


Subject(s)
Antimalarials/chemical synthesis , Vitamin K 3/analogs & derivatives , Vitamin K 3/chemical synthesis , Animals , Antimalarials/metabolism , Antimalarials/pharmacology , Carbon Isotopes , Drug Resistance, Multiple , Humans , Isotope Labeling , Mice , Oxidation-Reduction , Plasmodium berghei/drug effects , Plasmodium falciparum/drug effects , Vitamin K 3/metabolism , Vitamin K 3/pharmacology
10.
J Comput Aided Mol Des ; 31(5): 441-451, 2017 May.
Article in English | MEDLINE | ID: mdl-28374255

ABSTRACT

Generative topographic mapping (GTM) has been used to visualize and analyze the chemical space of antimalarial compounds as well as to build predictive models linking structure of molecules with their antimalarial activity. For this, a database, including ~3000 molecules tested in one or several of 17 anti-Plasmodium activity assessment protocols, has been compiled by assembling experimental data from in-house and ChEMBL databases. GTM classification models built on subsets corresponding to individual bioassays perform similarly to the earlier reported SVM models. Zones preferentially populated by active and inactive molecules, respectively, clearly emerge in the class landscapes supported by the GTM model. Their analysis resulted in identification of privileged structural motifs of potential antimalarial compounds. Projection of marketed antimalarial drugs on this map allowed us to delineate several areas in the chemical space corresponding to different mechanisms of antimalarial activity. This helped us to make a suggestion about the mode of action of the molecules populating these zones.


Subject(s)
Antimalarials/chemistry , Models, Molecular , Quantitative Structure-Activity Relationship , Databases, Factual , Drug Design , Humans , Molecular Conformation , Molecular Structure , Structure-Activity Relationship
11.
Molecules ; 22(1)2017 Jan 19.
Article in English | MEDLINE | ID: mdl-28106855

ABSTRACT

With the aim of increasing the structural diversity on the early antimalarial drug plasmodione, an efficient and versatile procedure to prepare a series of biaryl- and N-arylalkylamines as plasmodione analogues is described. Using the naturally occurring and commercially available menadione as starting material, a 2-step sequence using a Kochi-Anderson reaction and subsequent Pd-catalyzed Suzuki-Miyaura coupling was developed to prepare three representative biphenyl derivatives in good yields for antimalarial evaluation. In addition, synthetic methodologies to afford 3-benzylmenadione derivatives bearing a terminal -N(Me)2 or -N(Et)2 in different positions (ortho, meta and para) on the aryl ring of the benzylic chain of plasmodione were investigated through reductive amination was used as the optimal route to prepare these protonable N-arylalkylamine privileged scaffolds. The antimalarial activities were evaluated and discussed in light of their physicochemical properties. Among the newly synthesized compounds, the para-position of the substituent remains the most favourable position on the benzyl chain and the carbamate -NHBoc was found active both in vitro (42 nM versus 29 nM for plasmodione) and in vivo in Plasmodium berghei-infected mice. The measured acido-basic features of these new molecules support the cytosol-food vacuole shuttling properties of non-protonable plasmodione derivatives essential for redox-cycling. These findings may be useful in antimalarial drug optimization.


Subject(s)
Amines/administration & dosage , Amines/chemical synthesis , Antimalarials/administration & dosage , Antimalarials/chemical synthesis , Malaria/drug therapy , Amines/chemistry , Amines/pharmacology , Animals , Antimalarials/chemistry , Antimalarials/pharmacology , Combinatorial Chemistry Techniques , Mice , Molecular Structure , Oxidation-Reduction , Plasmodium berghei/drug effects , Structure-Activity Relationship , Vitamin K 3/analogs & derivatives
12.
Antimicrob Agents Chemother ; 60(9): 5146-58, 2016 09.
Article in English | MEDLINE | ID: mdl-27297478

ABSTRACT

Previously, we presented the chemical design of a promising series of antimalarial agents, 3-[substituted-benzyl]-menadiones, with potent in vitro and in vivo activities. Ongoing studies on the mode of action of antimalarial 3-[substituted-benzyl]-menadiones revealed that these agents disturb the redox balance of the parasitized erythrocyte by acting as redox cyclers-a strategy that is broadly recognized for the development of new antimalarial agents. Here we report a detailed parasitological characterization of the in vitro activity profile of the lead compound 3-[4-(trifluoromethyl)benzyl]-menadione 1c (henceforth called plasmodione) against intraerythrocytic stages of the human malaria parasite Plasmodium falciparum We show that plasmodione acts rapidly against asexual blood stages, thereby disrupting the clinically relevant intraerythrocytic life cycle of the parasite, and furthermore has potent activity against early gametocytes. The lead's antiplasmodial activity was unaffected by the most common mechanisms of resistance to clinically used antimalarials. Moreover, plasmodione has a low potential to induce drug resistance and a high killing speed, as observed by culturing parasites under continuous drug pressure. Drug interactions with licensed antimalarial drugs were also established using the fixed-ratio isobologram method. Initial toxicological profiling suggests that plasmodione is a safe agent for possible human use. Our studies identify plasmodione as a promising antimalarial lead compound and strongly support the future development of redox-active benzylmenadiones as antimalarial agents.


Subject(s)
Antimalarials/pharmacology , Gametogenesis/drug effects , Life Cycle Stages/drug effects , Naphthoquinones/pharmacology , Plasmodium falciparum/drug effects , Antimalarials/chemical synthesis , Artemisinins/pharmacology , Atovaquone/pharmacology , Drug Interactions , Drug Resistance/drug effects , Erythrocytes/drug effects , Erythrocytes/parasitology , Humans , Inhibitory Concentration 50 , Methylene Blue/pharmacology , Naphthoquinones/chemical synthesis , Plasmodium falciparum/growth & development
13.
Molecules ; 21(7)2016 Jun 29.
Article in English | MEDLINE | ID: mdl-27367660

ABSTRACT

Malaria is a parasitic tropical disease that kills around 600,000 patients every year. The emergence of resistant Plasmodium falciparum parasites to artemisinin-based combination therapies (ACTs) represents a significant public health threat, indicating the urgent need for new effective compounds to reverse ACT resistance and cure the disease. For this, extensive curation and homogenization of experimental anti-Plasmodium screening data from both in-house and ChEMBL sources were conducted. As a result, a coherent strategy was established that allowed compiling coherent training sets that associate compound structures to the respective antimalarial activity measurements. Seventeen of these training sets led to the successful generation of classification models discriminating whether a compound has a significant probability to be active under the specific conditions of the antimalarial test associated with each set. These models were used in consensus prediction of the most likely active from a series of curcuminoids available in-house. Positive predictions together with a few predicted as inactive were then submitted to experimental in vitro antimalarial testing. A large majority from predicted compounds showed antimalarial activity, but not those predicted as inactive, thus experimentally validating the in silico screening approach. The herein proposed consensus machine learning approach showed its potential to reduce the cost and duration of antimalarial drug discovery.


Subject(s)
Antimalarials/chemistry , Antimalarials/pharmacology , Computer Simulation , Data Mining , Drug Design , Plant Extracts/chemistry , Plant Extracts/pharmacology , Quantitative Structure-Activity Relationship , Curcuma/chemistry , Molecular Structure , Parasitic Sensitivity Tests , Plasmodium falciparum/drug effects
14.
Chemistry ; 21(8): 3415-24, 2015 Feb 16.
Article in English | MEDLINE | ID: mdl-25556761

ABSTRACT

In the context of the investigation of drug-induced oxidative stress in parasitic cells, electrochemical properties of a focused library of polysubstituted menadione derivatives were studied by cyclic voltammetry. These values were used, together with compatible measurements from literature (quinones and related compounds), to build and evaluate a predictive structure-redox potential model (quantitative structure-property relationship, QSPR). Able to provide an online evaluation (through Web interface) of the oxidant character of quinones, the model is aimed to help chemists targeting their synthetic efforts towards analogues of desired redox properties.

15.
ACS Infect Dis ; 10(5): 1808-1838, 2024 05 10.
Article in English | MEDLINE | ID: mdl-38606978

ABSTRACT

Chagas disease, or American trypanosomiasis, is a neglected tropical disease which is a top priority target of the World Health Organization. The disease, endemic mainly in Latin America, is caused by the protozoan Trypanosoma cruzi and has spread around the globe due to human migration. There are multiple transmission routes, including vectorial, congenital, oral, and iatrogenic. Less than 1% of patients have access to treatment, relying on two old redox-active drugs that show poor pharmacokinetics and severe adverse effects. Hence, the priorities for the next steps of R&D include (i) the discovery of novel drugs/chemical classes, (ii) filling the pipeline with drug candidates that have new mechanisms of action, and (iii) the pressing need for more research and access to new chemical entities. In the present work, we first identified a hit (4a) with a potent anti-T. cruzi activity from a library of 3-benzylmenadiones. We then designed a synthetic strategy to build a library of 49 3-(4-monoamino)benzylmenadione derivatives via reductive amination to obtain diazacyclic benz(o)ylmenadiones. Among them, we identified by high content imaging an anti-amastigote "early lead" 11b (henceforth called cruzidione) revealing optimized pharmacokinetic properties and enhanced specificity. Studies in a yeast model revealed that a cruzidione metabolite, the 3-benzoylmenadione (cruzidione oxide), enters redox cycling with the NADH-dehydrogenase, generating reactive oxygen species, as hypothesized for the early hit (4a).


Subject(s)
Chagas Disease , Oxidation-Reduction , Trypanocidal Agents , Trypanosoma cruzi , Trypanosoma cruzi/drug effects , Chagas Disease/drug therapy , Animals , Trypanocidal Agents/pharmacology , Trypanocidal Agents/chemistry , Trypanocidal Agents/chemical synthesis , Humans , Mice
16.
Antimicrob Agents Chemother ; 57(5): 2114-20, 2013 May.
Article in English | MEDLINE | ID: mdl-23439633

ABSTRACT

Methylene blue and a series of recently developed 1,4-naphthoquinones, including 3-[4-(substituted)benzyl]-menadiones, are potent antimalarial agents in vitro and in vivo. The activity of these structurally diverse compounds against the human malaria parasite Plasmodium falciparum might involve their peculiar redox properties. According to the current theory, redox-active methylene blue and 3-[4-(trifluoromethyl)benzyl]-menadione are "subversive substrates." These agents are thought to shuttle electrons from reduced flavoproteins to acceptors such as hemoglobin-associated or free Fe(III)-protoporphyrin IX. The reduction of Fe(III)-protoporphyrin IX could subsequently prevent essential hemoglobin digestion and heme detoxification in the parasite. Alternatively, owing to their structures and redox properties, methylene blue and 1,4-naphthoquinones might also affect the mitochondrial electron transport chain. Here, we tested the latter hypothesis using an established system of transgenic P. falciparum cell lines and the antimalarial agents atovaquone and chloroquine as controls. In contrast to atovaquone, methylene blue and 3-[4-(trifluoromethyl)benzyl]-menadione do not inhibit the mitochondrial electron transport chain. A systematic comparison of the morphologies of drug-treated parasites furthermore suggests that the three drugs do not share a mechanism of action. Our findings support the idea that methylene blue and 3-[4-(trifluoromethyl)benzyl]-menadione exert their antimalarial activity as redox-active subversive substrates.


Subject(s)
Antimalarials/pharmacology , Methylene Blue/pharmacology , Plasmodium falciparum/drug effects , Vitamin K 3/analogs & derivatives , Atovaquone/pharmacology , Chloroquine/pharmacology , Electron Transport/drug effects , Erythrocytes/drug effects , Erythrocytes/parasitology , Humans , Inhibitory Concentration 50 , Mitochondria/drug effects , Oxidation-Reduction/drug effects , Plasmodium falciparum/metabolism , Structure-Activity Relationship , Vitamin K 3/pharmacology
17.
Gastroenterology ; 143(1): 213-22.e5, 2012 Jul.
Article in English | MEDLINE | ID: mdl-22465429

ABSTRACT

BACKGROUND & AIMS: Interferon-based therapies for hepatitis C virus (HCV) infection are limited by side effects and incomplete response rates, particularly among transplant recipients. We screened a library of plant-derived small molecules to identify HCV inhibitors with novel mechanisms. METHODS: We isolated phenolic compounds from Marrubium peregrinum L (Lamiaceae). Replication of HCV RNA, virus production, and cell entry were monitored using replicons and infectious HCV. Inhibition of HCV was measured in hepatoma cells and primary human hepatocytes using luciferase reporter gene assays, core enzyme-linked immunosorbent assays, or infectivity titration. We tested the bioavailability of the compound in mice. RESULTS: We identified a flavonoid, ladanein (BJ486K), with unreported antiviral activity and established its oral bioavailability in mice. Natural and synthetic BJ486K inhibited a post-attachment entry step, but not RNA replication or assembly; its inhibitory concentration 50% was 2.5 µm. BJ486K was effective against all major HCV genotypes, including a variant that is resistant to an entry inhibitor; it prevented infection of primary human hepatocytes. Combined administration of BJ486K and cyclosporine A had a synergistic effect in inhibition of HCV infection. CONCLUSIONS: BJ486K has oral bioavailability and interferes with entry of HCV into cultured human hepatocytes. It synergizes with cyclosporine A to inhibit HCV infection. Its inhibitory effects are independent of HCV genotype, including a variant that is resistant to an entry inhibitor against scavenger receptor class B type I. Flavonoid derivatives therefore might be developed as components of combination therapies because they are potent, broadly active inhibitors of HCV entry that could prevent graft reinfection after liver transplantation.


Subject(s)
Antiviral Agents/pharmacology , Flavones/pharmacology , Hepacivirus , Hepatitis C/drug therapy , Hepatocytes/drug effects , Marrubium , Virus Internalization/drug effects , Cells, Cultured , Genotype , Humans , Phytotherapy , Plant Extracts/therapeutic use
18.
Org Biomol Chem ; 10(24): 4795-806, 2012 Jun 28.
Article in English | MEDLINE | ID: mdl-22618151

ABSTRACT

Menadione is the 2-methyl-1,4-naphthoquinone core used to design potent antimalarial redox-cyclers to affect the redox equilibrium of Plasmodium-infected red blood cells. Exploring the reactivity of fluoromethyl-1,4-naphthoquinones, in particular trifluoromenadione, under quasi-physiological conditions in NADPH-dependent glutathione reductase reactions, is discussed in terms of chemical synthesis, electrochemistry, enzyme kinetics, and antimalarial activities. Multitarget-directed drug discovery is an emerging approach to the design of new antimalarial drugs. Combining in one single 1,4-naphthoquinone molecule, the trifluoromenadione core with the alkyl chain at C-3 of the known antimalarial drug atovaquone, revealed a mechanism for CF(3) as a leaving group. The resulting trifluoromethyl derivative 5 showed a potent antimalarial activity per se against malarial parasites in culture.


Subject(s)
Antimalarials/chemical synthesis , Enzyme Inhibitors/chemical synthesis , Fluorine Compounds/chemical synthesis , Glutathione Reductase/antagonists & inhibitors , Vitamin K 3/chemical synthesis , Antimalarials/pharmacology , Biocatalysis , Drug Design , Enzyme Inhibitors/pharmacology , Fluorine Compounds/pharmacology , Humans , Molecular Structure , Oxidation-Reduction , Plasmodium falciparum/drug effects , Plasmodium falciparum/enzymology , Structure-Activity Relationship , Vitamin K 3/pharmacology
19.
Org Biomol Chem ; 10(31): 6375-87, 2012 Aug 21.
Article in English | MEDLINE | ID: mdl-22777178

ABSTRACT

Improving the solubility of polysubstituted 1,4-naphthoquinone derivatives was achieved by introducing nitrogen in two different positions of the naphthoquinone core, at C-5 and at C-8 of menadione through a two-step, straightforward synthesis based on the regioselective hetero-Diels-Alder reaction. The antimalarial and the antischistosomal activities of these polysubstituted aza-1,4-naphthoquinone derivatives were evaluated and led to the selection of distinct compounds for antimalarial versus antischistosomal action. The Ag(II)-assisted oxidative radical decarboxylation of the phenyl acetic acids using AgNO(3) and ammonium peroxodisulfate was modified to generate the 3-picolinyl-menadione with improved pharmacokinetic parameters, high antimalarial effects and capacity to inhibit the formation of ß-hematin.


Subject(s)
Antimalarials/chemistry , Naphthoquinones/chemistry , Plasmodium falciparum/drug effects , Quinolines/chemistry , Schistosoma mansoni/drug effects , Schistosomicides/chemistry , Animals , Antimalarials/chemical synthesis , Antimalarials/pharmacology , Hemin/antagonists & inhibitors , Hemin/metabolism , Humans , Malaria, Falciparum/drug therapy , Methemoglobin/metabolism , Mice , Naphthoquinones/chemical synthesis , Naphthoquinones/pharmacology , Quinolines/chemical synthesis , Quinolines/pharmacology , Schistosomiasis mansoni/drug therapy , Schistosomicides/chemical synthesis , Schistosomicides/pharmacology , Solubility
20.
Eur J Med Chem ; 243: 114735, 2022 Dec 05.
Article in English | MEDLINE | ID: mdl-36122550

ABSTRACT

A series of ferrocenyl-containing γ-hydroxy-γ-lactam tetramates were prepared in 2-3 steps through ring opening-ring closure (RORC) process of γ-ylidene-tetronate derivatives in the presence of ferrocenyl alkylamines. The compounds were screened in vitro for their antiplasmodial activity against chloroquine-sensitive (3D7) and chloroquine-resistant (W2) clones of P. falciparum, displaying activity in the range of 0.12-100 µM, with generally good resistance index. The most active ferrocene in these series exhibited IC50 equal to 0.09 µM (3D7) and 0.12 µM (W2). The low cytotoxicity of the ferrocenyl-containing γ-hydroxy-γ-lactam tetramates against Human Umbilical Vein Endothelial (HUVEC) cell line demonstrated selective antiparasitic activity. The redox properties of these ferrocene-derived tetramates were studied and physico-biochemical studies evidenced that these derivatives can exert potent antimalarial activities via a mechanism distinct from ferroquine.


Subject(s)
Antimalarials , Malaria, Falciparum , Humans , Metallocenes/pharmacology , Antimalarials/chemistry , Plasmodium falciparum , Lactams/pharmacology , Lactams/chemistry , Structure-Activity Relationship , Malaria, Falciparum/drug therapy , Chloroquine/therapeutic use
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