Profile of commercialized aphicides on the survivorship and feeding behavior of the cotton aphid, Aphis gossypii.

The cotton aphid, Aphis gossypii Glover (Hemiptera: Aphididae), is one of the most destructive agricultural pests due to photosynthate removal and horizontal transmission of plant viruses. Horizontal transmission of plant viruses by aphids occurs during distinct feeding behavioral events, such as probing for non-persistent viruses or phloem feeding for persistent viruses. We employed toxicity bioassays and electrical penetration graph (EPG) methodology to compare toxicity and quantify changes to feeding behavior and toxicity of A. gossypii after exposure to commercialized aphicides. Commercialized aphicides containing flupyradifurone, sulfoxaflor, thiamethoxam, thiamethoxam + lambda cyhalothrin, and bifenthrin induced >90% aphid mortality within 4 h of exposure. Flupyradifurone was the most acutely toxic aphicide studied with an LT50 of 8.9 min after exposure, which was approximately 3-fold lower than bifenthrin and thiamethoxam + lambda cyhalothrin. This was supported by our EPG results that showed a significant reduction in the proportion of aphids that continued to probe on cotton 4 h after exposure to flonicamid, thiamethoxam, flupyradifurone, bifenthrin, and thiamethoxam + lambda cyhalothrin. The commercialized aphicides containing spirotetramat, flonicamid, thiamethoxam, flupyradifurone, bifenthrin, sulfoxaflor, and pymetrozine significantly (P < 0.05) decreased the time to first probe when compared to the untreated control. Lastly, E1 (phloem salivation) and E2 (phloem ingestion) waveforms were significantly (P < 0.05) reduced for flupyradifurone, flonicamid, thiamethoxam, sulfoxaflor, and thiamethoxam. These data provide a comparative study for the development of new aphicides aiming to induce acute lethality and reduce aphid transmission of plant viruses.

The Lipid Flippases ALA4 and ALA5 Play Critical Roles in Cell Expansion and Plant Growth.

Aminophospholipid ATPases (ALAs) are lipid flippases involved in transporting specific lipids across membrane bilayers. Arabidopsis (Arabidopsis thaliana) contains 12 ALAs in five phylogenetic clusters, including four in cluster 3 (ALA4-ALA7). ALA4/5 and ALA6/7, are expressed primarily in vegetative tissues and pollen, respectively. Previously, a double knockout of ALA6/7 was shown to result in pollen fertility defects. Here we show that a double knockout of ALA4/5 results in dwarfism, characterized by reduced growth in rosettes (6.5-fold), roots (4.3-fold), bolts (4.5-fold), and hypocotyls (2-fold). Reduced cell size was observed for multiple vegetative cell types, suggesting a role for ALA4/5 in cellular expansion. Members of the third ALA cluster are at least partially interchangeable, as transgenes expressing ALA6 in vegetative tissues partially rescued ala4/5 mutant phenotypes, and expression of ALA4 transgenes in pollen fully rescued ala6/7 mutant fertility defects. ALA4-GFP displayed plasma membrane and endomembrane localization patterns when imaged in both guard cells and pollen. Lipid profiling revealed ala4/5 rosettes had perturbations in glycerolipid and sphingolipid content. Assays in yeast revealed that ALA5 can flip a variety of glycerolipids and the sphingolipid sphingomyelin across membranes. These results support a model whereby the flippase activity of ALA4 and ALA5 impacts the homeostasis of both glycerolipids and sphingolipids and is important for cellular expansion during vegetative growth.

In-phase sub-Nyquist lenslet arrays encoded onto spatial light modulators.

When encoding diffractive lenses onto a spatial light modulator (SLM), there is a Nyquist limit to the smallest focal length that can be formed. When this limit is surpassed, a two-dimensional array of lenslets is formed. There have been very few discussions on the performance of these lenslets. In this work, we focus on the phase distribution of these lenses in the array. We show that, for certain values of the focal length, the lenslets are all in perfect phase. We show that this situation happens for a total number of N/4 different discrete equidistant sub-Nyquist focal lengths, where N×N is the number of pixels in the SLM. We find other distances in between where the array is composed of two sets of lenslets with a relative π phase among them. Finally, we illustrate these phase distributions in the application to generate an array of vortex producing lenses. We expect that these results might be useful for high-accuracy interferometric or multiple imaging where this phase must be exactly the same for each replica.

Gouy phase effects on propagation of pure and hybrid vector beams.

The robustness of the polarization spatial distribution of vector beams upon propagation is crucial for a number of applications, including optical communications and materials processing. This study has been commonly centered on Gouy phase effects on focused vector beams. In this work, we present a theoretical and experimental analysis of the Gouy phase's effects on the propagation of pure and hybrid vector beams. Experimental results at various axial planes, before and past the focus, are obtained by using a simplified liquid-crystal spatial light modulator-based optical system that allows the easy generation of these beams. Furthermore, a new alternative optical set-up that is devoid of moving elements is demonstrated, which simplifies this study. We experimentally verify the differences between pure and hybrid vector beams upon propagation. While the first ones remain stable, hybrid vector beams show Gouy phase effects that demonstrate an optical activity where the local polarization states rotate by an angle that depends on the propagation distance. Experimental results agree with the theory.

Encoding lenses with focal lengths lower than the Nyquist limit using high phase-modulation displays.

Liquid crystal displays allow the easy implementation of diffractive optical elements. However, the shortest focal lengths for lenses are limited by Nyquist conditions. In this work, we show that focal lengths much lower than this Nyquist limit can be encoded onto devices having a large phase-dynamic range. Experimental results are included with a display showing 10π phase modulation reducing the Nyquist limit by a factor of about 1/10.

Simulation of multimode optical fibers using the angular spectrum algorithm and a Fourier analysis.

Multimode optical fibers are gaining increased interest for higher data rate transmissions. In this work, we examine the propagation of beams in multimode optical fibers using the angular spectrum method for several values for the propagation constant V. We prepare a sequence of 4096 images over 200,000 steps, each representing a wavelength within the fiber. We perform a one-dimensional Fourier transform of these data and obtain the propagation constant for each transmitted mode. We then obtain the electric field profile for each transmitted mode. We find excellent agreement with weakly guided mode theory. This work represents another step in the use of this algorithm for analyzing more complicated optical fiber structures.

Simulation of optical fiber couplers using the angular spectrum algorithm.

We examine single-mode optical fiber transmission using the angular spectrum method. We find excellent agreement with the theoretical solutions for the cylindrical single-mode optical fiber. Next, we examine fiber coupler configurations with 1×2, 1×3, and 1×5 designs. The input within the central fiber core is broadcast equally into the surrounding fiber cores and then returns with propagation distances as expected. Then we examine supermode theory for the 1×3 case. The initial energy is sent into different combinations of fiber cores where their phase relationship governs the output. Surprisingly, we find evidence of an unexpected mode in some of the multicore designs. We expect these results might be useful for exploration of more complicated fiber core arrays. However, computational times are short using this algorithm.

Q-plates with a nonlinear azimuthal distribution of the principal axis: application to encoding binary data.

We encode q-plates where the angular orientation of the principal axis is varied spatially with a nonstandard distribution. In the usual q-plate design, the orientation of the optical axis depends linearly on the azimuthal angle. In this work, we examine cases where this azimuthal dependence is nonlinear. We consider two cases: first, where the principal axis distribution is like an inverse-tangent function of the azimuth; and second, where it displays linear and flat segments. This last case is proposed as a new method for encoding binary data into the azimuthal lobes of the vector beam. We encode these patterns using a spatial light modulator system that allows new and exotic q-plate designs without the difficulty of fabricating individual plates. Experimental results are presented.

Arithmetic with q-plates.

In this work we show the capability to form various q-plate equivalent systems using combinations of commercially available q-plates. We show operations like changing the sign of the q-value, or the addition and subtraction of q-plates. These operations only require simple combinations of q-plates and half-wave plates. Experimental results are presented in all cases. Following this procedure, experimental testing of higher and negative q-valued devices can be carried out using commonly available q-valued devices.

Diffraction gratings generating orders with selective states of polarization.

We propose specially designed double anisotropic polarization diffraction gratings capable of producing a selective number of diffraction orders and with selective different states of polarization. Different polarization diffraction gratings are demonstrated, including linear polarization with horizontal, vertical and ± 45° orientations, and circular R and L polarization outputs. When illuminated with an arbitrary state of polarization, the system acts as a complete polarimeter where the intensities of the diffraction orders allow measurement of the Stokes parameters with a single shot. Experimental proof-of-concept is presented using a parallel-aligned liquid crystal display operating in a double pass architecture.

Terahertz thermometry of gold nanospheres in water.

The photo-thermal effects of plasmonic nanoparticles are promising for cancer therapies. These treatments would greatly benefit from real-time, multi-scale temperature mapping by non-invasive means. Here we show that intense terahertz time domain spectroscopy can be used as a non-contact and high-resolution thermometer of water solutions. Using this technique, we measure the temperature change, triggered by femtosecond amplified laser pulses, of a solution of gold nanospheres in water. Extensions of this ultra-fast and non-invasive technique could open the door to real-time micro-thermometry of single cells without fluorescent labels.

Nondiffracting vector beams where the charge and the polarization state vary with propagation distance.

We generate nondiffracting vector beams where the charge and the polarization state vary with the propagation distance. We use reflective geometry where a parallel-aligned spatial light modulator is used to spatially modulate two orthogonal linear polarizations. We encode spiral phases with equal charge but with opposite signs onto the two polarization directions to encode a vector beam and add two axicon phases. Both the charge and the phase shift between the two axicons can be varied along the focus line. We provide experimental results that demonstrate both features.

Generation of integer and fractional vector beams with q-plates encoded onto a spatial light modulator.

We generate programmable vector beams with arbitrary q-plates encoded using a spatial light modulator system. Consequently, we can analyze new and exotic q-plate designs without the difficulty of fabricating individual plates. We show experimental results for positive and negative integer and new fractional vector beam values.

Performance of a q-plate tunable retarder in reflection for the switchable generation of both first- and second-order vector beams.

We examine the performance of a tunable liquid crystal q-plate in a reflective geometry. When the device is tuned to a half-wave retardance, it operates as a q-plate with twice the value (2q) by adding a quarter-wave retarder between the mirror and the q-plate. However, when the device is tuned to a quarter-wave retardance, it acts as the original q-plate without the retarder. Experimental results are shown. Using an input tunable polarization state generator, the system allows the switchable production of all states on both the first- and second-order Poincaré spheres.

Revealing and Characterizing Dark Excitons through Coherent Multidimensional Spectroscopy.

Dark excitons are of fundamental importance in a broad range of contexts but are difficult to study using conventional optical spectroscopy due to their weak interaction with light. We show how coherent multidimensional spectroscopy can reveal and characterize dark states. Using this approach, we identify parity-forbidden and spatially indirect excitons in InGaAs/GaAs quantum wells and determine details regarding lifetimes, homogeneous and inhomogeneous linewidths, broadening mechanisms, and coupling strengths. The observations of coherent coupling between these states and bright excitons hint at a role for a multistep process by which excitons in the barrier can relax into the quantum wells.

Azimuthal multiple-beam interference effects with combinations of vortex beams.

We create a series of vortex beams consisting of positive and negative topological charges and develop a geometry where they interfere creating azimuthal multiple-beam interference effects. Usually the combination of two beams with opposite charges creates an azimuthal two-beam interference effect where the intensity varies sinusoidally with azimuthal angle. We combine several of these beams to create patterns where the interference becomes more sharply defined in the azimuthal direction and where destructive interference eliminates some of the intensity peaks. The process is complicated by the fact that the radii of the different vortex beams depend on the topological charge and the focal length of the Fourier lens. We generate a series of patterns where the different charges are focused with different focal lengths such that their radii agree. However to encode these, we encode patterns from the lower charges that have been Fresnel diffracted to the plane of the highest charge. These complicated patterns are encoded onto a liquid crystal display (LCD). Experimental results agree well with theory.

Nondiffracting Bessel beams with polarization state that varies with propagation distance.

We generate nondiffracting Bessel beams whose polarization state varies with propagation distance. We use a reflective geometry where a single parallel-aligned spatial light modulator device is used to spatially modulate two orthogonal linear polarizations with two axicon phase profiles. Then, by adding an extra phase retardation radial profile between these linear states, we are able to modulate the state of polarization along the line focus of the axicon. We provide experimental results that demonstrate the polarization axial control with zero-order and higher order Bessel beams.

Analysis of a segmented q-plate tunable retarder for the generation of first-order vector beams.

In this work we study a prototype q-plate segmented tunable liquid crystal retarder device. It shows a large modulation range (5π rad for a wavelength of 633 nm and near 2π for 1550 nm) and a large clear aperture of one inch diameter. We analyze the operation of the q-plate in terms of Jones matrices and provide different matrix decompositions useful for its analysis, including the polarization transformations, the effect of the tunable phase shift, and the effect of quantization levels (the device is segmented in 12 angular sectors). We also show a very simple and robust optical system capable of generating all polarization states on the first-order Poincaré sphere. An optical polarization rotator and a linear retarder are used in a geometry that allows the generation of all states in the zero-order Poincaré sphere simply by tuning two retardance parameters. We then use this system with the q-plate device to directly map an input arbitrary state of polarization to a corresponding first-order vectorial beam. This optical system would be more practical for high speed and programmable generation of vector beams than other systems reported so far. Experimental results are presented.

Isolating quantum coherence using coherent multi-dimensional spectroscopy with spectrally shaped pulses.

We demonstrate how spectral shaping in coherent multidimensional spectroscopy can isolate specific signal pathways and directly access quantitative details. By selectively exciting pathways involving a coherent superposition of exciton states we are able to identify, isolate and analyse weak coherent coupling between spatially separated excitons in an asymmetric double quantum well. Analysis of the isolated signal elucidates details of the coherent interactions between the spatially separated excitons. With a dynamic range exceeding 10(4) in electric field amplitude, this approach facilitates quantitative comparisons of different signal pathways and a comprehensive description of the electronic states and their interactions.

Accelerating light beams with arbitrarily transverse shapes.

Accelerating beams are wave packets that preserve their shape while propagating along curved trajectories. Their unique characteristics have opened the door to applications that range from optical micromanipulation and plasma-channel generation to laser micromachining. Here, we demonstrate, theoretically and experimentally, that accelerating beams can be generated with a variety of arbitrarily chosen transverse shapes. We present a general method to construct such beams in the paraxial and nonparaxial regime and demonstrate experimentally their propagation in the paraxial case. The key ingredient of our method is the use of the spectral representation of the accelerating beams, which offers a unique and compact description of these beams. The on-demand accelerating light patterns described here are likely to give rise to new applications and add versatility to the current ones.