ABSTRACT
OBJECTIVE: Injury to the sternoclavicular joint is a rare phenomenon that has implications for the shoulder complex. Limited literature exists on optimal physical therapy rehabilitation after a sternoclavicular ligament sprain. This case report details the physical therapy management and outcomes of a patient with a posterior sternoclavicular joint sprain. CASE DESCRIPTION: The patient was a 34-year-old female who sustained a grade I posteriorly directed sternoclavicular sprain during a motor vehicle accident. She received a combination of acromioclavicular joint mobilizations and therapeutic exercise for her shoulder complex for five sessions over six weeks. OUTCOMES: At discharge, the patient surpassed the minimally clinically important difference (MCID) and the minimal detectable change (MDC) in her Quick-DASH score. She surpassed the MDC and MCID in her Numerical Pain Rating Scale score. She was able to regain full functional use of her involved upper extremity and returned to her work and original exercise regimen with no further limitations. CONCLUSION: The outcomes suggest that a combination of acromioclavicular joint mobilizations and therapeutic exercise for the shoulder complex was a suitable option for the conservative management of this patient's grade I sternoclavicular sprain.
ABSTRACT
Recent findings have highlighted the role of the Old World alphavirus non-structural protein 3 (nsP3) as a host defence modulator that functions by disrupting stress granules, subcellular phase-dense RNA/protein structures formed upon environmental stress. This disruption mechanism was largely explained through nsP3-mediated recruitment of the host G3BP protein via two tandem FGDF motifs. Here, we present the 1.9 Å resolution crystal structure of the NTF2-like domain of G3BP-1 in complex with a 25-residue peptide derived from Semliki Forest virus nsP3 (nsP3-25). The structure reveals a poly-complex of G3BP-1 dimers interconnected through the FGDF motifs in nsP3-25. Although in vitro and in vivo binding studies revealed a hierarchical interaction of the two FGDF motifs with G3BP-1, viral growth curves clearly demonstrated that two intact FGDF motifs are required for efficient viral replication. Chikungunya virus nsP3 also binds G3BP dimers via a hierarchical interaction, which was found to be critical for viral replication. These results highlight a conserved molecular mechanism in host cell modulation.
Subject(s)
DNA Helicases/chemistry , DNA Helicases/metabolism , Poly-ADP-Ribose Binding Proteins/chemistry , Poly-ADP-Ribose Binding Proteins/metabolism , RNA Helicases/chemistry , RNA Helicases/metabolism , RNA Recognition Motif Proteins/chemistry , RNA Recognition Motif Proteins/metabolism , RNA-Binding Proteins/chemistry , RNA-Binding Proteins/metabolism , Semliki forest virus/physiology , Viral Nonstructural Proteins/chemistry , Viral Nonstructural Proteins/metabolism , Amino Acid Motifs , Cell Line , Chikungunya virus/metabolism , Chikungunya virus/physiology , Conserved Sequence , Crystallography, X-Ray , HEK293 Cells , Humans , Models, Molecular , Protein Binding , Protein Multimerization , Protein Structure, Tertiary , Semliki forest virus/metabolism , Virus ReplicationABSTRACT
The extended pi-delocalization of dppn (benzo[i]dipyrido[3,2-a:2,3-c]phenazine) results in a (3)pipi* state as the lowest triplet excited state in [Os(bpy)(2)(dppn)](2+) (bpy = 2,2'-bipyridine), which generates a (1)O(2) quantum yield of 0.42. Together with its (3)MLCT absorption, this new osmium complex shows efficient DNA cleavage under irradiation of lambda(irr) > or = 645 nm.
Subject(s)
Coordination Complexes/chemistry , DNA Cleavage , DNA/chemistry , Osmium/chemistry , 2,2'-Dipyridyl/chemistry , Coordination Complexes/chemical synthesis , DNA/radiation effects , Molecular Conformation , Quantum TheoryABSTRACT
The population and reactivity of two low-lying excited states in [Ru(bpy)(2)(dppn)](2+) (bpy = 2,2'-bipyridine, dppn = benzo[i]dipyrido[3,2-a:2',3'-c]phenazine), a weakly emissive (3)MLCT state and a long-lived ligand-centered (3)pipi* state, lead to efficient photoinduced DNA damage. Irradiation with visible light results in nearly complete DNA cleavage within 30 s (lambda(irr) > or = 455 nm), likely from the combined action of guanine oxidation and the production of reactive oxygen species derived from (1)O(2).
Subject(s)
Coordination Complexes/chemistry , DNA/chemistry , Light , Organometallic Compounds/chemistry , Ruthenium/chemistry , DNA/radiation effects , DNA Cleavage , Guanine/chemistry , Oxidation-Reduction , Photolysis , Reactive Oxygen Species/metabolismABSTRACT
Oral cancer affects approximately 5% of the Canadian population every year. One option for treatment of oropharyngeal cancer includes resection of the diseased tissue with primary reconstruction of the defect using a microvascular free flap, followed by post-operative adjuvant radiation therapy. The aim of reconstructive surgery is to maintain functional speech and swallowing. While the literature provides support for the maintenance of speech intelligibility following reconstructive procedures, certain aspects of resonance may be altered when the palatal structures are involved. Little is known about the effect of such alterations on the perception of speakers who have been treated with microvascular free flap reconstruction. Social perception is a process in which we infer attributes of others, with the speech signal playing an integral part in attribution. The purpose of this study was to explore the social perceptions formed about speakers both before and after surgery for oropharyngeal cancer. The results of this study revealed that positive perceptions of speakers significantly diminished as a result of surgery and negative perceptions increased. Certain variables, such as degree of resection of the soft palate and base of tongue, and sex of the speaker, had influence on the results. This research suggests that intelligibility measurements of speech, although useful, do not provide a complete indication of the social impact of reconstructive surgery on patients with oropharyngeal resections.