Ameliorative impacts of gamma-aminobutyric acid (GABA) on seedling growth, physiological biomarkers, and gene expression in eight wheat (Triticum aestivum L.) cultivars under salt stress.

Plants spontaneously accumulate γ-aminobutyric acid (GABA), a nonprotein amino acid, in response to various stressors. Nevertheless, there is limited knowledge regarding the precise molecular mechanisms that plants employ to cope with salt stress. The objective of this study was to investigate the impact of GABA on the salt tolerance of eight distinct varieties of bread wheat (Triticum aestivum L.) by examining plant growth rates and physiological and molecular response characteristics. The application of salt stress had a detrimental impact on plant growth markers. Nevertheless, the impact was mitigated by the administration of GABA in comparison to the control treatment. When the cultivars Gemmiza 7, Gemmiza 9, and Gemmiza 12 were exposed to GABA at two distinct salt concentrations, there was a substantial increase in both the leaf chlorophyll content and photosynthetic rate. Both the control wheat cultivars and the plants exposed to salt treatment and GABA treatment showed alterations in stress-related biomarkers and antioxidants. This finding demonstrated that GABA plays a pivotal role in mitigating the impact of salt treatments on wheat cultivars. Among the eight examined kinds of wheat, CV. Gemmiza 7 and CV. Gemmiza 11 exhibited the most significant alterations in the expression of their TaSOS1 genes. CV. Misr 2, CV. Sakha 94, and CV. Sakha 95 exhibited the highest degree of variability in the expression of the NHX1, DHN3, and GR genes, respectively. The application of GABA to wheat plants enhances their ability to cope with salt stress by reducing the presence of reactive oxygen species (ROS) and other stress indicators, regulating stomatal aperture, enhancing photosynthesis, activating antioxidant enzymes, and upregulating genes involved in salt stress tolerance.

Hypoalbuminemia affects the spatio-temporal tissue distribution of ochratoxin A in liver and kidneys: consequences for organ toxicity.

Hypoalbuminemia (HA) is frequently observed in systemic inflammatory diseases and in liver disease. However, the influence of HA on the pharmacokinetics and toxicity of compounds with high plasma albumin binding remained insufficiently studied. The 'lack-of-delivery-concept' postulates that HA leads to less carrier mediated uptake of albumin bound substances into hepatocytes and to less glomerular filtration; in contrast, the 'concept-of-higher-free-fraction' argues that increased concentrations of non-albumin bound compounds facilitate hepatocellular uptake and enhance glomerular filtration. To address this question, we performed intravital imaging on livers and kidneys of anesthetized mice to quantify the spatio-temporal tissue distribution of the mycotoxin ochratoxin A (OTA) based on its auto-fluorescence in albumin knockout and wild-type mice. HA strongly enhanced the uptake of OTA from the sinusoidal blood into hepatocytes, followed by faster secretion into bile canaliculi. These toxicokinetic changes were associated with increased hepatotoxicity in heterozygous albumin knockout mice for which serum albumin was reduced to a similar extent as in patients with severe hypoalbuminemia. HA also led to a shorter half-life of OTA in renal capillaries, increased glomerular filtration, and to enhanced uptake of OTA into tubular epithelial cells. In conclusion, the results favor the 'concept-of-higher-free-fraction' in HA; accordingly, HA causes an increased tissue uptake of compounds with high albumin binding and increased organ toxicity. It should be studied if this concept can be generalized to all compounds with high plasma albumin binding that are substrates of hepatocyte and renal tubular epithelial cell carriers.

Immunomodulatory Efficacy-Mediated Anti-HCV and Anti-HBV Potential of Kefir Grains; Unveiling the In Vitro Antibacterial, Antifungal, and Wound Healing Activities.

The utilization of fermented foods with health-promoting properties is becoming more popular around the world. Consequently, kefir, a fermented milk beverage made from kefir grains, was shown in numerous studies to be a probiotic product providing significant health benefits. Herein, we assessed the antibacterial and antifungal potential of kefir against a variety of pathogenic bacteria and fungi. This study also showed the effectiveness of kefir in healing wounds in human gastric epithelial cells (GES-1) by (80.78%) compared with control (55.75%) within 48 h. The quantitative polymerase chain reaction (qPCR) results of kefir-treated HCV- or HBV- infected cells found that 200 µg/mL of kefir can eliminate 92.36% of HCV and 75.71% of HBV relative to the untreated infected cells, whereas 800 µg/mL (the highest concentration) completely eradicated HCV and HBV. Moreover, the estimated IC50 values of kefir, at which HCV and HBV were eradicated by 50%, were 63.84 ± 5.81 µg/mL and 224.02 ± 14.36 µg/mL, correspondingly. Kefir can significantly suppress the elevation of TNF-α and upregulate IL-10 and INF-γ in both treated HCV- and HBV-infected cells. High-performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS) analysis of kefir revealed the presence of numerous active metabolites which mainly contribute to the antimicrobial, antiviral, and immunomodulatory activities. This study demonstrated, for the first time, the anti-HBV efficacy of kefir while also illustrating the immunomodulatory impact in the treated HBV-infected cells. Accordingly, kefir represents a potent antiviral agent against both viral hepatitis C and B, as well as having antimicrobial and wound healing potential.

γ-Aminobutyric acid (GABA) mitigates drought and heat stress in sunflower (Helianthus annuus L.) by regulating its physiological, biochemical and molecular pathways.

Drought and heat stress are two dominant abiotic stress factors that often occur simultaneously in nature causing oxidative damage in plants and thus decline in yield. The present study was conducted to examine the γ-aminobutyric acid (GABA)-induced heat and drought tolerance in sunflower through physiological, biochemical and molecular analysis. The results showed that drought and heat stress triggered oxidative stress as revealed by enhanced level in hydrogen peroxide, malondialdehyde and electrolyte leakage. Moreover, the photosynthetic attributes such as photosynthetic rate, stomatal conductance and quantum efficiency declined when subjected to drought and heat stress. In this study, GABA treatment effectively alleviated the drought and heat-induced stress as reflected by significantly higher levels of proline, soluble sugar and total protein content. Besides, the data also revealed the direct relationship between antioxidant enzyme activities (superoxide dismutase, peroxidase, glutathione reductase, monodehydroascorbate peroxidase, ascorbate peroxidase) and the relative expression of genes (Heat Shock Proteins, Dehydrin, Osmotin, Aquaporin, Leaf Embryogenesis Protein), under drought and heat stress. Moreover, a significant increase in gene expression was observed upon GABA treatment with respect to control. This data suggest that GABA-induced drought and heat tolerance in sunflower could involve the improvement in osmolyte metabolism, gene expression and antioxidant enzyme activities and thus a rise in the GABA shunt which in turn provides intermediates during long-term drought and heat stress, thus maintaining homeostasis.

Comparative Physicochemical Evaluation of Starch Extracted from Pearl millet seeds grown in Sudan as a Pharmaceutical Excipient against Maize and Potato Starch, using Paracetamol as a model drug.

OBJECTIVES: The aim of the current study was to compare the physicochemical and disintegrant properties of pearl millet starch with other starches using paracetamol as model drug. METHODOLOGY: Determination of percentage yield, Physicochemical, micrometrics characteristics of starch/granules, drug excipients compatibility studies and evaluation of prepared paracetamol tablets were measured using official techniques. RESULTS: The yield of the millet starch ranged from 30 to 40%. Moisture content 8.77%, pH 5.7, Swelling capacity 1.2, Hydration capacity 1.748, Moisture uptake 11.8%, Amylose 24.6%, with poor flowability and compressibility. No significant difference in hardness, friability% & disintegration times for formulations containing millet starch to that containing potato and maize starch (P>0.05). CONCLUSION: From the study, Millet seeds locally cultivated in Sudan gave a high yield of starch, has same physicochemical properties as maize and potato starch so can be used as an alternative to those starches.

Effect of intravenous alteplase on ischaemic lesion water homeostasis.

BACKGROUND AND PURPOSE: Intravenous (IV) lysis with alteplase is known to increase biomarkers of blood-brain barrier breakdown and has therefore been associated with secondary injuries such as hemorrhagic transformation. The impact of alteplase on brain edema formation, however, has not been investigated yet. The purpose was to examine the effects of IV alteplase on ischaemic lesion water homeostasis differentiated from final tissue infarct in patients with and without successful endovascular therapy (sET). METHODS: In all, 232 middle cerebral artery stroke patients were analyzed. 147 patients received IV alteplase, of whom 106 patients received subsequent sET. Out of 85 patients without IV alteplase, 50 received sET. Ischaemic brain edema was quantified at admission and follow-up computed tomography using quantitative lesion net water uptake (NWU) and its difference was calculated (ΔNWU). The relationship of alteplase on ΔNWU and edema-corrected final infarct volume was analyzed using univariate and multivariate linear regression models. RESULTS: The mean ΔNWU was 11.8% (SD 7.9) in patients with alteplase and 11.5% (SD 8.3) in patients without alteplase (P = 0.8). Alteplase was not associated with lowered ΔNWU whilst being associated with reduced edema-corrected tissue infarct volume [-27.4 ml, 95% confidence interval (CI) -49.4 to -5.4 ml; P = 0.02], adjusted for the Alberta Stroke Program Early Computed Tomography Score and recanalization status. In patients with sET, ΔNWU was 10.5% (95% CI 6.3%-10.5%) for patients with IV alteplase and 8.4% (95% CI 9.1%-12.0%) for patients without IV alteplase. CONCLUSION: The application of IV alteplase did not significantly alter ischaemic lesion water homeostasis but was associated with reduced edema-corrected tissue infarct volume, which might be directly linked to improved functional outcome.

Radiographic evaluation and determination of hypercementosis patterns in Al-Madinah Al-Munawwarah, Saudi Arabia: A retrospective cross-sectional study.

OBJECTIVES: Hypercementosis (HC) is an asymptomatic excessive cementum deposition at the dental root apex. There is a lack of research that determines the pattern and associated factors of HC in Saudi Arabia. MATERIALS AND METHODS: A retrospective cross-sectional study was conducted on a random sample of 815 Orthopantomograms (OPGs) that was obtained from a total of 14003 female patients archived OPGs. The OPGs were retrieved from the Kodak Carestream-R4 database for the period between 2015 and 2017 at Taibah University Dental College and Hospital (TUDCH), Al-Madinah Al-Munawwarah, Saudi Arabia. Width and radiodensity of HC were measured and the distribution was calculated. Association between HC and factors including age and nationality were explored. Descriptive and inferential analyses were performed. RESULTS: The mean/SD age of HC affected patients (9.8%) was 30.16 ± 13.1 years, of whom 55% were Saudis. The mean/SD width and radiodensity of the hypercementosed roots were 4.14 ± 1.2 mm and 119.86 ± 29.83, respectively. The mandibular teeth were more frequently associated with hypercementosis (65%) specifically first molars (47.5%), while the majority (64%) had a single unilateral distribution pattern. There was a non-significant association between nationality, age, and HC (P = 0.921, P = 0.633, respectively). CONCLUSION: Hypercementosis observed in 9.8% of female patients attending TUDCH which was primarily unilateral and prominently confined to the mandibular teeth roots. The complication of teeth extraction among female patients in Almadinah region should be anticipated.

Efficacy of balance exercises intervention on postural control-related impairment in children with sensorineural hearing loss.

BACKGROUND: Sensorineural hearing loss is the most common type of permanent hearing impairment and results in postural control and motor deficits in children that may affect or delay all developmental indicators. OBJECTIVE: The purpose of the study was to investigate the efficacy of balance exercises intervention on postural control-related impairment in children with sensorineural hearing loss. METHODS: Forty students of both genders, ages ranging from 10 to 16 years, diagnosed with severe to profound sensorineural hearing loss, were selected from the Public School for the Deaf and Hard of Hearing in El-Minia district, Minia governorate, Egypt. They divided randomly into two groups, 20 (study group), received balance exercises in addition to their ordinary daily living activities. Meanwhile, the control group of 20 children practiced only the ordinary daily living activities. The outcome was assessed pre-treatment and post-treatment by Humac Balance System and Bruininks-Oseretsky Test (BOT-2) subtest (5) for balance. RESULTS: Regarding the Humac balance system and subtest (5) of BOT-2, there was a statistically significant difference between pre-treatment data and post-treatment data of the study group with a p value equal to 0.036 or less. However, no statistically significant difference was observed in the control group with a p value equal to 0.096 or more. Finally, there was a statistically significant difference between the groups with respect to the post-treatment data, where the p value was 0.014 or less. CONCLUSION: Postural control of children with sensorineural hearing loss has been improved by balance exercises.

Accuracy of the revised Tokuhashi score in predicting survival in patients with metastatic spinal cord compression (MSCC).

PURPOSE: The revised Tokuhashi score has been widely used to evaluate indications for surgery and predict survival in patients with metastatic spinal disease. Our aim was to analyse the actual survival time of patients treated for metastatic spinal cord compression (MSCC) in comparison with the predicted survival based on the revised Tokuhashi score. This would thereby allow us to determine the overall predictive value of this scoring system. METHODS: This study was a semi-prospective clinical study of all patients with MSCC presenting to our unit over 8 years-data from October 2003 to December 2009 were collected retrospectively and from December 2009, all data collected prospectively to October 2011. Patients were divided into three groups--Group 1 (Tokuhashi score 0-8, n = 84), Group 2 (Tokuhashi score 9-11, n = 83) and Group 3 (Tokuhashi score 12-15, n = 34). Data collected included demographic data, primary tumour histology, surgery type and complications, neurological outcome (Frankel grade) and survival. RESULTS: A total of 233 patients with MSCC were managed surgically in our unit during this time. Out of these complete data were available on 201 patients for analysis. Mean age of patients was 61 years (range 18-86; 127 M, 74 F). The primary tumour type was Breast (n = 29, 15 %), Haematological (n = 28, 14 %), Renal (n = 26, 13 %), Prostate (n = 26, 13 %), Lung (n = 23, 11 %), Gastro-intestinal (n = 11, 5 %), Sarcoma (n = 9, 4 %) and others (n = 49, 24 %). All patients included in the study had surgical intervention in the form of decompression and stabilisation. Posterior decompression and stabilisation was performed in 171 patients (with vertebrectomy in 31), combined anterior and posterior approaches were used in 18 patients and 12 had an anterior approach only. The overall complication rate was 19 % (39/201)--the most common being wound infection (n = 15, 8 %). There was no difference in the neurological outcome (Frankel grade) between Groups 1 and 2 (p = 0.34) or Groups 2 and 3 (p = 0.70). However, there was a significant difference between Groups 1 and 3 (p = 0.001), with Group 3 having a significantly better neurological outcome. Median survival was 93 days in Group 1, 229 days in Group 2 and 875 days in Group 3 (p = 0.001). The predictive value between the actual and predicted survival was 64 % (Group 1), 64 % (Group 2) and 69 % (Group 3). The overall predictive value of the revised Tokuhashi score using Cox regression for all groups was 66 %. CONCLUSION: We would conclude that although the predictive value of the Tokuhashi score in terms of survival time is at best modest (66 %), the fact that there were statistically significant differences in survival between the groups looked at in this paper indicates that the scoring system, and the components which it consists of, are important in the evaluation of these patients when considering surgery.

Effect of timing of surgery on neurological outcome and survival in metastatic spinal cord compression.

PURPOSE: Metastatic spinal cord compression (MSCC) requires expeditious treatment. While there is no ambiguity in the literature about the urgency of care for patients with MSCC, the effect of timing of surgical intervention has not been investigated in detail. The objective of our study was to investigate whether or not the 'timing of surgery' is an important factor in survival and neurological outcome in patients with MSCC. METHODS: All patients with MSCC presenting to our unit from October 2005 to March 2010 were included in this study. Patients were divided into three groups-those who underwent surgery within 24 h (Group 1, n = 45), between 24 and 48 h (Group 2, n = 23) and after 48 h (Group 3, n = 53) from acute presentation of neurological symptoms. The outcome measures studied were neurological outcome (change in Frankel grade post-operatively), survival (survival rate and median survival in days), incidence of infection, length of stay and complications. RESULTS: Patients' age, gender, revised Tokuhashi score, level of spinal metastasis and primary tumour type were not significantly different between the three groups. Greatest improvement in neurology was observed in Group 1, although not significantly when compared against Group 2 (24-48 h; (p = 0.09). When comparisons of neurological outcome were performed for all patients having surgery within 48 h (Groups 1 and 2) versus after 48 h (Group 3), the Frankel grade improvement was significant (p = 0.048) favouring surgery within 48 h of presentation. There was a negative correlation (-0.17) between the delay in surgery and the immediate neurological improvement, suggesting less improvement in those who had delayed surgery. There was no difference in length of hospital stay, incidence of infection, post-operative complications or survival between the groups. CONCLUSIONS: Our results show that surgery should be performed sooner rather than later. Furthermore, earlier surgical treatment within 48 h in patients with MSCC resulted in significantly better neurological outcome. However, the timing of surgery did not influence length of hospital stay, complication rate or patient survival.

Effect of ultrasound-enhanced bee venom on selected post inguinal hernioplasty complications: a single-blind randomized controlled trial.

OBJECTIVE: Bee venom (BV) phonophoresis has been recommended as a non-invasive treatment for a variety of inflammatory conditions and for reducing post-operative pain. This study aimed at evaluating the impact of bee venom phonophoresis around incisions and on selected acupuncture points for the treatment of pain, inflammation, and mobility of the hip following inguinal hernioplasty. PATIENTS AND METHODS: Sixty-six male patients who had acute pain and decreased mobility of the hip after having an indirect unilateral inguinal hernioplasty with a mesh participated in this study. Patients were randomly assigned into two equal groups of 33. The bee venom phonophoresis group (Group A) received low-intensity pulsed ultrasound using BV gel, and the control group (Group B) received low-intensity pulsed ultrasound using only plain gel without BV gel. Both groups received the same regular medical care and 5 minutes of therapy each, three times a week, for three weeks postoperative. The visual analogue scale (VAS), serum C-reactive protein (CRP), and hip ROM measurements were used to assess the acute pain, inflammation, and ROM pre-application (pre-treatment) and post-3 weeks of treatment (post-treatment) for both groups. RESULTS: The findings have exhibited an extremely significant difference in VAS, CRP, and hip ROM measurements in the BV phonophoresis group compared to that of the control group (p < 0.05). CONCLUSIONS: BV delivered by phonophoresis around incisions and on selected acupuncture points has a beneficial effect in the treatment of pain, inflammation, and mobility of the hip following inguinal hernioplasty with mesh.

A cross-sectional analysis of the association between diabetes self-care practices and blood glucose regulation among Egyptian diabetic patients.

OBJECTIVE: Diabetes Mellitus (DM) is a main public health issue worldwide, with Egypt among the world's top countries with diabetic patients. Data on the degree of self-care behaviors of Egyptian diabetic patients is not fully reported. Therefore, the study aimed to assess the adequacy of diabetes self-management (DSM) habits among diabetic patients in Egypt and to identify associated socio-demographic factors that could negatively impact the patients' glycemic state, using a structured questionnaire. SUBJECTS AND METHODS: This study was a cross-sectional observational study. The diabetes self-management questionnaire (DSMQ) was used to assess diabetes self-care activities, which is a 16-item questionnaire. The statistical analysis was conducted on SPSS software, and the data were significant with a p-value <0.05. RESULTS: A total of 2,164 participants were included. The majority of the patients (83.8%) had type 2 DM. The mean glycated hemoglobin (HbA1c) of the study group was 8.44±1.99. Significant differences were found between different glycemic control groups both in terms of the DSMQ sum scores and the subscale scores, with the exception of physical activity. Moreover, there was a significant weak inverse correlation between the total DSMQ sum score and HbA1c ≥9.0% (ρ=-0.116, p<0.01). The overall internal consistency (Cronbach's alpha) was acceptable (0.756). CONCLUSIONS: The study indicated that patients with controlled blood glucose had significantly higher 'glucose management', and 'total DSMQ sum' scores compared to uncontrolled diabetic patients. All significant correlations between assessed parameters and DSMQ were weak.

Sublingual floor of Rahmani sheep (Ovis aries): A scanning electron microscopy and histomorphology analysis.

The current study investigated the sublingual floor of Rahmani sheep (Ovis aries). Samples from nine healthy adult sheep have been analysed using morphometrical, scanning electron microscopic (SEM), and histological examination. The sublingual floor of the sheep was formed of a rostral prefrenular part (spatula-shaped) and two lateral sublingual recesses. Right and left sublingual caruncles were asymmetrical in length and each one had a serrated border, divided into three papillae. At higher magnifications, the three papillae of the sublingual caruncle's edge were variable in shape, with other two papillae were demonstrated underneath the caruncle. Each sublingual recess had a sublingual fold (plica sublingualis) extended from the level of frenulum linguae till the glossopalatine arch, bordered with caudolaterally directed papillae till the level of first lower molar cheek teeth. The sublingual salivary glands were mixed (seromucoid) type. The glandular lobule consisted of mucous acini and sporadic serous acini, as well as the presence of some mucous acini capped by acidophilic cells, serous demilunes. The mucous acinus was formed of pyramidal mucous cells with pale stained cytoplasm and basally located nuclei. Two ducts; major sublingual and submandibular ducts were observed within the submucosa of the sublingual caruncle. The sublingual ductal pseudostratified columnar epithelium was interspersed by more goblet cells than that of the submandibular duct. Collagen fibres around the glandular lobules, intralobular and interlobular excretory ducts as well as the connective core of the papillae were noticed. By SEM, two epithelium-lined pits on the surface of the sublingual floor mucosa caudal to the central incisive teeth were termed orobasal organ. As the first study to give a comprehensive analysis of the sheeps' sublingual floor, this one serves as a baseline for future clinical studies.

Combined effect of Schroth method and Gensingen brace on Cobb's angle and pulmonary functions in adolescent idiopathic scoliosis: a prospective, single blinded randomized controlled trial.

OBJECTIVE: To detect the effect of the Schroth method added to the Gensingen brace for six months on Cobb's angle, axial rotation of the trunk, and pulmonary function in adolescent idiopathic scoliosis. PATIENTS AND METHODS: The study followed a prospective, single-blinded, randomized controlled trial design following the CONSORT guidelines. The study was conducted in Health and Rehabilitation Centre. 42 males aged 10-18 years old, diagnosed with adolescent idiopathic scoliosis (AIS) with curves of 35-40°, were recruited and then were allocated into three groups: Group A, Group B, and Group C. RESULTS: Significant improvement (p < 0.001) in Cobb's angle and the axial rotation of the trunk (ART) were noted after the treatment in the three groups, while pulmonary function showed better results in Group A (p < 0.001) when compared to the two other groups p ≥ 0.000). CONCLUSIONS: Six months of Schroth method added to Gensingen Brace demonstrate favorable results in Cobb's angle, thoracic trunk rotation angle, and pulmonary function in adolescent idiopathic scoliosis.

Marginal gap distance and cyclic fatigue loading for different all-ceramic endocrowns.

OBJECTIVE: The aim of the study was to evaluate the effect of the fabrication techniques of two types of glass ceramics on the marginal gap distance and the fracture resistance of endocrown restorations after cyclic loading. MATERIALS AND METHODS: Forty extracted mandibular first molars were root canal treated. Decoronation was done for all the endodontically treated teeth 2 mm above the cemento-enamel junction. The teeth were individually fixed vertically into epoxy resin mounting cylinders. All teeth were prepared to receive endocrown restorations. The prepared teeth were randomly divided into four equal groups (n=10) according to the all-ceramic materials and technique used for endocrown construction as follows: Group I (n=10): Pressable lithium disilicate glass ceramics (IPS e-max Press), Group II (n=10): Pressable zirconia-reinforced lithium disilicate glass ceramics (Celtra Press), Group III (n=10): Machinable lithium disilicate glass ceramics (IPS e-max CAD), Group IV (n=10): Machinable zirconia-reinforced lithium disilicate glass ceramics (Celtra Duo). The endocrowns were cemented using dual-cure resin cement. All endocrowns were subjected to fatigue loading. The cycles were repeated 120,000 times to clinically simulate one year chewing condition. Marginal gap distance of all endocrowns was measured directly using a digital microscope with x100 magnification. The load required to failure was recorded in Newton. Data were collected, tabulated, and statistically analyzed. RESULTS: Fracture resistance testing of all-ceramic crowns revealed a statistically significant difference between all different ceramic materials used in this study (p-value <0.001). On the other hand, there was a statistically significant difference between all the four ceramic crowns for the marginal gap distance either before or after fatigue cyclic loading. CONCLUSIONS: After considering the limitation of the current study, the following conclusions were given: endocrowns are considered one of the promising minimally invasive restorations for root canal treated molars. CAD/CAM technology revealed better results than heat press technology regarding the fracture resistance of glass ceramics. Heat Press technology revealed better results than CAD/CAM technology regarding the marginal accuracy of glass ceramics.

Retraction Note: Combined effect of Schroth method and Gensingen brace on Cobb's angle and pulmonary functions in adolescent idiopathic scoliosis: a prospective, single blinded randomized controlled trial.

The article "Combined effect of Schroth method and Gensingen brace on Cobb's angle and pulmonary functions in adolescent idiopathic scoliosis: a prospective, single blinded randomized controlled trial", by S.A. Moawd, G. Nambi, A.E. El-Bagalaty, S.M. Hassan, S.E.B. Elsayed, F.M. Aboelmagd, N.A. Alhwoaimel, H.A. Abdeen, published in Eur Rev Med Pharmacol Sci 2023; 27 (2): 601-610-DOI: 10.26355/eurrev_202301_31061-PMID: 36734703 has been retracted by the Editor in Chief for the following reasons: After publication, concerns were raised about the use of copyright and trademark protected terms "Gensingen Brace" and "the Schroth Method" by the authors of the paper without permission for referring to results of a study with treatment of patients with a "Gensingen Brace". The owner of the copyright expressly denies the use of his trademark for scientific statements that are neither authorized by him nor correspond to the product's characteristics and qualities of his well-known products sold under the trademark. After being informed about the copyright and trademark issue, the corresponding author has been acknowledged and affirmed that the authors were not aware of the possible copyright and trademark infringement at the time of submission. The Publisher apologizes for any inconvenience this may cause. https://www.europeanreview.org/article/31061.

Validity and reliability of Squegg device in measuring isometric handgrip strength.

OBJECTIVE: The quantitative measurement of handgrip strength is important in assessing and charting the progress of patients with neuromuscular diseases. The aim of this research was to determine the intra-rater and inter-rater reliability and the validity of the Squegg digital dynamometer. SUBJECTS AND METHODS: Twenty-one females and nine male participants with an age range between 18 and 40 years volunteered for the study. Three testers each took three measurements with a Squegg device and a Jamar dynamometer using standardized measurement techniques. Intra- and inter-tester reliability were calculated using the intra-class correlation coefficient (ICC). To investigate the relationship between hand measures and isometric handgrip strength, the Pearson correlation coefficient test was used. To determine the agreement between the two devices, a Bland Altman plot was constructed, and the concurrent validity of Squegg was calculated. RESULTS: The intra-rater reliability coefficients for both Jamar and Squegg were greater than 0.99 for all three testers, indicating excellent intra-rater reliability. The inter-rater reliability of Jamar (ICC=0.93) and Squegg (ICC=0.87) was excellent. With an ICC of 0.844 and an r-value of 0.720, Squegg with Jamar demonstrates good validity and statistical significance (p=0.001). CONCLUSIONS: The isometric handgrip strength and hand measures showed a moderate correlation in the study population. The Squegg isometric handgrip dynamometer has good concurrent validity and great intra- and inter-rater reliability in healthy individuals. The validity of Squegg in patients with neuromuscular diseases that affect hand function has to be investigated further.

Retention of different all ceramic endocrown materials cemented with two different adhesive techniques.

OBJECTIVE: The purpose of this in vitro study was to evaluate the influence of two adhesive techniques on the retentive force of four all ceramic endocrowns. MATERIALS AND METHODS: Forty maxillary first molars of approximately similar size and shape were collected. The teeth were all decoronated 2 mm above the level of proximal cement-enamel junction (CEJ) and were all endodontically treated. The teeth were then randomly divided equally into four groups (10 each) according to all ceramic material used, as follows: Group I (VE) - Ten prepared molars were restored with hybrid ceramic (Vita Enamic); Group II (LU) - Ten prepared molars were restored with resin Nano-ceramic (Lava Ultimate). Group III (CD) - Ten prepared molars were restored with zirconia-reinforced lithium di-silicate ceramic material (Celtra Duo); Group IV (LZ) - Ten prepared molars were restored with zirconia ceramic (Lava Zirconia). Each group was then subdivided into two equal subgroups (n=5) according to the type of cement (adhesive technique) used for cementation. Subgroup A (RX ARC): the endocrowns were cemented with a total-etch adhesive resin cement (RelyX ARC). Subgroup B (RXU): the endocrowns were cemented with self-adhesive resin luting cement (RelyX UniCem). The restorations were designed with an outer cylindrical handle located on buccal and palatal surfaces to provide a mean for the removal of the endocrowns during the pull-out testing. The cemented endocrowns were thermocycled and then removed along the path of insertion using a universal testing machine at 0.5 mm/min. The retentive force was recorded, and the stress of dislodgement was calculated using the surface area of each preparation. RESULTS: The highest mean dislodgement stresses were 64.3 MPa for Group I (VE), whereas there was no statistically significant difference between Group I, II and III and LZ showed the lowest values with significant difference between the other three groups. Regarding the type of cement, there was a statistically significant difference between RelyX ARC (mean=60.09 MPa) and RelyX Unicem (mean=49.73 MPa). CONCLUSIONS: Retention of Vita Enamic, Lava Ultimate, and Celtra Duo are significantly higher than Lava Zirconia.

Effect of two fascial incision options for access to the temporomandibular joint on facial nerve function: objective investigation.

This study was performed to compare the effects on facial nerve (FN) function of the deep supra-temporalis muscle subfascial approach (DSFA) and traditional fascial approach (TFA) for access to the temporomandibular joint (TMJ), via qualitative and quantitative evaluations. Thirty patients requiring open TMJ surgery were randomly allocated to one of two groups: group A patients underwent the DSFA approach, while group B patients underwent the TFA approach. The TMJ was accessed via modified endaural incision with temporal extension. Clinical examinations, FN conduction tests, and electromyography (EMG) of the frontalis and orbicularis oculi muscles were used to assess FN function. A FN function deficit was noted in 50% of the whole sample population immediately after surgery, with no statistically significant difference between the groups (P = 0.082). Overall, 37.5% of the total study population experienced temporary loss of frontalis muscle activity, while zygomatic nerve injury was seen only in 25% of group A. Within 2-6 months, normal function returned in both groups. Nerve conduction studies showed no statistically significant difference between the groups in terms of nerve amplitude or latency after surgery. However, EMG of orbicularis oculi activity showed a significant difference between the groups after 6 months (P = 0.010). The results suggest that the traditional dissection approach is more protective of the FN, especially the zygomatic branch, than the deeper dissection technique.

Analyzing the influence of the combination of monochromatic infrared energy and tai chi exercise improve balance in community-dwelling older adults with lower-extremity disease: a double-blinded randomized controlled study.

OBJECTIVE: This study aimed to evaluate the efficacy of a combination of MIRE exposure and Tai Chi exercise on balance and fall risk in community-dwelling older adults with lower-extremity disease (LED). PATIENTS AND METHODS: In this double-blinded randomized controlled study, 49 older adults were randomly allocated into the MIRE group (n=25), in which the participants were exposed to 30 min of MIRE and performed 40-45 min of Tai Chi exercise, three sessions/week; and the sham group (n=24), in which the participants only performed the Tai Chi exercise. We measured the ankle/brachial index (ABI), as well as scores of the Berg balance scale (BBS), Tinetti clinical scale (TCS), and Timed Up and Go Test (TUG) before and after 3-months of intervention. RESULTS: A significant increase in ABI and scores of BBS, and TCS, was observed, along with a significant decrease in TUG scores in the MIRE group. The sham group showed a significant increase in the ABI, and TCS score, and a significant decrease in the TUG score, with a non-significant change in the BBS score. Comparison between both study groups illustrated that the patients in the MIRE group experienced improved balance and decreased fall risk. CONCLUSIONS: Three months of MIRE exposure and Tai Chi exercise improved balance and reduced the risk of falls in community-dwelling older adults with LEDs.