Near-infrared hyperspectral imaging to map collagen content in prehistoric bones for radiocarbon dating.

Many of the rarest prehistoric bones found by archaeologists are enormously precious and are considered to be part of our cultural and historical patrimony. Radiocarbon dating is a well-established technique that estimates the ages of bones by analysing the collagen still present. However, this method is destructive, and its use must be limited. In this study, we used imaging technology to quantify the presence of collagen in bone samples in a non-destructive way to select the most suitable samples (or sample regions) to be submitted to radiocarbon dating analysis. Near-infrared spectroscopy (NIR) that was connected to a camera with hyperspectral imaging (HSI) was used along with a chemometric model to create chemical images of the distribution of collagen in ancient bones. This model quantifies the collagen at every pixel and thus provides a chemical mapping of collagen content. Our results will offer significant advances for the study of human evolution as we will be able to minimise the destruction of valuable bone material, which is under the protection and enhancement of European cultural heritage and thus allow us to contextualise the valuable object by providing an accurate calendar age.

γ9δ2 T-Cell Expansion and Phenotypic Profile Are Reflected in the CDR3δ Repertoire of Healthy Adults.

γ9δ2T cells fill a distinct niche in human immunity due to the unique physiology of the phosphoantigen-reactive γ9δ2TCR. Here, we highlight reproducible TCRδ complementarity-determining region 3 (CDR3δ) repertoire patterns associated with γ9δ2T cell proliferation and phenotype, thus providing evidence for the role of the CDR3δ in modulating in vivo T-cell responses. Features that determine γ9δ2TCR binding affinity and reactivity to the phosphoantigen-induced ligand in vitro appear to similarly underpin in vivo clonotypic expansion and differentiation. Likewise, we identify a CDR3δ bias in the γ9δ2T cell natural killer receptor (NKR) landscape. While expression of the inhibitory receptor CD94/NKG2A is skewed toward cells bearing putative high-affinity TCRs, the activating receptor NKG2D is expressed independently of the phosphoantigen-sensing determinants, suggesting a higher net NKR activating signal in T cells with TCRs of low affinity. This study establishes consistent repertoire-phenotype associations and justifies stratification for the T-cell phenotype in future research on γ9δ2TCR repertoire dynamics.

A new miniaturised short-wave infrared (SWIR) spectrometer for on-site cultural heritage investigations.

The paper reports on the development of an analytical method based on the use of a new miniaturised short-wave infrared (SWIR) spectrometer for the analysis of cultural heritage samples. The spectrometer is a prototype characterised by small dimension (45.0 mm in diameter x 47.5 mm in height x 60 g weight), easily handled and transferable out of research laboratories. The prototype enables the acquisition of spectra in the SWIR range of 1200-2200 nm, which is a unique feature for miniaturised spectrometers. The exploitation of this spectral range allows the detection of a high number of combination and overtone bands, which guarantees significant diagnostic power to the instrument. The present study lays a significant foundation to the development of analytical strategies based on miniaturised NIR spectrometers working in the SWIR spectral range for the characterization of complex samples such as cultural heritage specimens. Analytical performances of the new spectrometer were assessed on archaeological bones, cinematographic films and bronze patinas. The selected cases of study present challenging conservation issues not properly addressed, and their analyses usually require to be performed on-site, in places not easily accessible by restorers, archaeologists and/or scientists. The data acquired with the prototype, combined with a multivariate data analysis approach, show the possibility to i) differentiate between the materials used as a support for cinematographic film namely cellulose nitrate (CN), cellulose acetates (CA) and polyethylene terephthalate (PET); ii) sort out archaeological bone fragments according to their collagen content as an initial screening test for bones characterization; iii) differentiate between corrosion products on outdoor bronze sculpture, which is important for assessing the state of conservation of the artwork. The prototype enabled rapid information acquisition to guide restoration strategies, which need to be supported in real time by quick and easy analytical procedures.