ABSTRACT
Okibacterium fritillariae gen. nov., sp. nov. (type strain VKM Ac-2059T = IFO 16404T) is proposed for aerobic, oxidase- and catalase-positive, coryneform bacteria isolated from seeds of Fritillaria ruthenica Wikstr. and Clematis recta L. Strains of the new genus form a distinct branch within the phylogenetic cluster composed of members of the family Microbacteriaceae and are characterized by B-type peptidoglycan containing amino acids glycine, glutamate, homoserine, alanine and lysine, the glycolyl type of muramic acid, the major menaquinones MK-10 and MK-11, the principal phospholipids phosphatidylglycerol and diphosphatidylglycerol, and a DNA G+C content of approximately 67 mol %.
Subject(s)
Actinomycetales/classification , Clematis/microbiology , Fritillaria/microbiology , Seeds/microbiology , Actinomycetales/chemistry , Actinomycetales/genetics , Actinomycetales/physiology , Bacterial Typing Techniques , DNA, Ribosomal/analysis , Molecular Sequence Data , Peptidoglycan/analysis , Peptidoglycan/chemistry , Phospholipids , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNA , Vitamin K 2/analysisABSTRACT
The cell wall of Brevibacterium sp. VKM Ac-2118 isolated from a frozen (mean annual temperature -12 degrees C) late Pliocene layer, 1.8-3 Myr, Kolyma lowland, Russia, contains mannitol teichoic acid with a previously unknown structure. This is 1,6-poly(mannitol phosphate) with the majority of the mannitol residues bearing side phosphate groups at O-4(3). The structure of the polymer was established by chemical methods, NMR spectroscopy, and MALDI-TOF mass spectrometry.