ABSTRACT
Studies have been made on viability and hereditary properties of the progeny of amoebae after micrurgical coercions (sectioning of cells, suction of cytoplasm) during metaphase and early anaphase. The progeny of the fragments as well as of the cells with the reduced bulk of the cytoplasm exhibits unusual methionine resistance. Possible mechanism of the observed phenomemon are discussed.
Subject(s)
Amoeba/physiology , Clone Cells/drug effects , Genetics , Mitosis , Animals , Ethanol/pharmacology , Methionine/pharmacology , MicromanipulationABSTRACT
17 mouse cell lines have been screened with specific sera against H-2 antigens. All the cell lines tested expressed H-2 antigens characteristic of the donor haplotype. The data obtained indicate that H-2 typing of cultured mouse cells can be used as an approach to control their intraspecies diversity.
Subject(s)
Cells, Cultured/classification , H-2 Antigens/classification , Animals , Cell Line , Fluorescent Antibody Technique , H-2 Antigens/analysis , Haplotypes , Immune Sera/immunology , Mice , Mice, Inbred Strains , Species SpecificityABSTRACT
Suspension of splenocytes of control C3HA mice and mice examined early after transplantation of mouse hepatoma 22a cells were fractionated by treatment with anti-T-sera (anti-thymocytes, antibrain and anti-suppressor-T-cells). This treatment leads to various changes in NK-activity due to elimination of different subpopulations of T-lymphocytes. This variability may be associated with the presence of two or more types of suppressor cells able to influence the NK-cells directly or by means of other immunocompetent cells.
Subject(s)
Antilymphocyte Serum/pharmacology , Killer Cells, Natural/drug effects , Liver Neoplasms, Experimental/immunology , Mice, Inbred C3H/immunology , Spleen/drug effects , T-Lymphocytes/immunology , Animals , Brain/immunology , Killer Cells, Natural/immunology , Male , Mice , Neoplasm Transplantation , Spleen/immunology , T-Lymphocytes, Regulatory/immunology , Time FactorsABSTRACT
A single injection of C3HA mice with various immunomodulators-ds-RNA, thymogene (TM) and cyclophosphamide (CY)--performed one day before transplantation of syngeneic hepatoma 22a cells led to a decrease in the tumor growth rate. The most prominent effect was found following the CY treatment. The NK cell activity estimated per spleen of mice treated with ds-RNA and TM was seen increased in comparison with the control mice not given the modulators. The rate of tumor growth was due probably to this fact. The protective effect of CY may be accounted for by a direct action of this agent on tumor cells.
Subject(s)
Adjuvants, Immunologic/pharmacology , Dipeptides , Killer Cells, Natural/drug effects , Liver Neoplasms, Experimental/immunology , Mice, Inbred C3H/immunology , Spleen/drug effects , Adjuvants, Immunologic/therapeutic use , Animals , Cyclophosphamide/pharmacology , Cyclophosphamide/therapeutic use , Cytotoxicity Tests, Immunologic , Drug Screening Assays, Antitumor , Killer Cells, Natural/immunology , Liver Neoplasms, Experimental/drug therapy , Male , Mice , Neoplasm Transplantation , Peptides/pharmacology , Peptides/therapeutic use , RNA, Double-Stranded/pharmacology , RNA, Double-Stranded/therapeutic use , Spleen/immunology , Time FactorsABSTRACT
The induction of tumor in C3HA mice after intramuscular injection with 20-methylcholanthrene is accompanied by a decrease in natural antitumor resistance. This conclusion is based on the observation of the decreased natural killer activity per total number of splenocytes, from the time of carcinogen application till the appearance of tumor nodes.
Subject(s)
Killer Cells, Natural/drug effects , Methylcholanthrene/toxicity , Mice, Inbred C3H/immunology , Neoplasms, Experimental/chemically induced , Spleen/drug effects , Animals , Cell Count/drug effects , Cytotoxicity, Immunologic/drug effects , Killer Cells, Natural/immunology , Male , Mice , Neoplasms, Experimental/immunology , Organ Size/drug effects , Spleen/immunology , Time FactorsABSTRACT
The influence of macrophages on NK cell activity of C3HA mice on 1, 7 and 13 days after single i. m. injection of 20-methylcholanthren was investigated. It is shown that macrophages significantly stimulate this activity on the 13th day after carcinogen application.
Subject(s)
Killer Cells, Natural/immunology , Macrophages/immunology , Methylcholanthrene/toxicity , Neoplasms, Experimental/immunology , Spleen/immunology , Animals , Cell Adhesion/immunology , Cells, Cultured/immunology , Cytotoxicity Tests, Immunologic , Male , Mice , Mice, Inbred C3H , Neoplasms, Experimental/chemically induced , Time FactorsABSTRACT
The role of T-lymphocytes in natural cytotoxicity of splenocytes of C3HA mice after a single injection of 20-methylcholanthrene (20-MC) was investigated. A splenocyte suspension was treated with anti-T-cell serum and complement. This treatment was not shown to exert influence on the natural cytotoxicity of splenocytes within 1-13 days after injecting 20-MC.