Nano-pulsed discharge plasma-induced abiotic oligopeptide formation from diketopiperazine.

Conventional oligopeptide synthesis techniques involve environmentally harmful procedures and materials. In addition, the efficient accumulation of oligopeptides under Hadean Earth environments regarding the origin of life remains still unclear. In these processes, the formation of diketopiperazine is a big issue due to the strong inhibition for further elongation beyond dipeptides. Hydrothermal media enables environmentally friendly oligopeptide synthesis. However, hydrothermal oligopeptide synthesis produces large amounts of diketopiperazine (DKP), due to its thermodynamic stability. DKP inhibits dipeptide elongation and also constitutes an inhibitory pathway in conventional oligopeptide synthesis. Here, we show an efficient pathway for oligopeptide formation using a specially designed experimental setup to run both thermal and non-thermal discharge plasma, generated by nano-pulsed electric discharge with 16-23 kV voltage and 300-430 A current within ca. 500 ns. DKP (14%) was converted to dipeptides and higher oligopeptides in an aqueous solution containing alanine-DKP at pH 4.5, after 20 min of 50 pps thermal plasma irradiation. This is the first study to report efficient oligopeptide synthesis in aqueous medium using nano-pulsed plasma (with thermal plasma being more efficient than non-thermal plasma) via DKP ring-opening. This unexpected finding is implicative to evaluate the pathway how the oligopeptides could have accumulated in the primitive Earth with high-energy plasma sources such as thunder as well as to facilitate the green synthesis of oligopeptides.

Oral Supplementation with Z-Isomer-Rich Astaxanthin Inhibits Ultraviolet Light-Induced Skin Damage in Guinea Pigs.

The effect of oral supplementation with astaxanthin of different Z-isomer ratios on ultraviolet (UV) light-induced skin damage in guinea pigs was investigated. Astaxanthin with a high Z-isomer content was prepared from the all-E-isomer via thermal isomerization. Intact (all-E)-astaxanthin and the prepared Z-isomer-rich astaxanthin were suspended in soybean oil and fed to guinea pigs for three weeks. The UV-light irradiation was applied to the dorsal skin on the seventh day after the start of the test diet supplementation, and skin parameters, such as elasticity, transepidermal water loss (TEWL), and pigmentation (melanin and erythema values), were evaluated. The accumulation of astaxanthin in the dorsal skin was almost the same after consumption of the all-E-isomer-rich astaxanthin diet (E-AST-D; total Z-isomer ratio = 3.2%) and the Z-isomer-rich astaxanthin diet (Z-AST-D; total Z-isomer ratio = 84.4%); however, the total Z-isomer ratio of astaxanthin in the skin was higher in the case of the Z-AST-D supplementation. Both diets inhibited UV light-induced skin-damaging effects, such as the reduction in elasticity and the increase in TEWL level. Between E-AST-D and Z-AST-D, Z-AST-D showed better skin-protective ability against UV-light exposure than E-AST-D, which might be because of the greater UV-light-shielding ability of astaxanthin Z-isomers than the all-E-isomer. Furthermore, supplementation with Z-AST-D resulted in a greater reduction in skin pigmentation caused by astaxanthin accumulation compared to that of E-AST-D. This study indicates that dietary astaxanthin accumulates in the skin and appears to prevent UV light-induced skin damage, and the Z-isomers are more potent oral sunscreen agents than the all-E-isomer.

Improved Carotenoid Processing with Sustainable Solvents Utilizing Z-Isomerization-Induced Alteration in Physicochemical Properties: A Review and Future Directions.

Carotenoids-natural fat-soluble pigments-have attracted considerable attention because of their potential to prevent of various diseases, such as cancer and arteriosclerosis, and their strong antioxidant capacity. They have many geometric isomers due to the presence of numerous conjugated double bonds in the molecule. However, in plants, most carotenoids are present in the all-E-configuration. (all-E)-Carotenoids are characterized by high crystallinity as well as low solubility in safe and sustainable solvents, such as ethanol and supercritical CO2 (SC-CO2). Thus, these properties result in the decreased efficiency of carotenoid processing, such as extraction and emulsification, using such sustainable solvents. On the other hand, Z-isomerization of carotenoids induces alteration in physicochemical properties, i.e., the solubility of carotenoids dramatically improves and they change from a "crystalline state" to an "oily (amorphous) state". For example, the solubility in ethanol of lycopene Z-isomers is more than 4000 times higher than the all-E-isomer. Recently, improvement of carotenoid processing efficiency utilizing these changes has attracted attention. Namely, it is possible to markedly improve carotenoid processing using safe and sustainable solvents, which had previously been difficult to put into practical use due to the low efficiency. The objective of this paper is to review the effect of Z-isomerization on the physicochemical properties of carotenoids and its application to carotenoid processing, such as extraction, micronization, and emulsification, using sustainable solvents. Moreover, aspects of Z-isomerization methods for carotenoids and functional difference, such as bioavailability and antioxidant capacity, between isomers are also included in this review.

The thermal Z-isomerization-induced change in solubility and physical properties of (all-E)-lycopene.

The effect of Z-isomerization of (all-E)-lycopene on its solubility in organic solvents and physical properties was investigated. Lycopene samples containing different Z-isomer contents (23.8%, 46.9%, and 75.6% of total lycopene) were prepared from high-purity (all-E)-lycopene by thermal Z-isomerization in dichloromethane (CH2Cl2). As the Z-isomer content increased, the relative solubility of lycopene significantly improved. Although (all-E)-lycopene barely dissolved in ethanol (0.6 mg/L), the solubilities of lycopene containing 23.8%, 46.9%, and 75.6% Z-isomers were 484.5, 914.7, and 2401.7 mg/L, respectively. Furthermore, differential scanning calorimetry (DSC), powder X-ray diffraction (XRD), and scanning electron microscopy (SEM) analyses clearly indicated that (all-E)-lycopene was present in the crystal state, while Z-isomers of lycopene were present in amorphous states. A number of studies have suggested that Z-isomers of lycopene are better absorbed in the human body than the all-E-isomer. This may be due to the change in solubility and physical properties of lycopene by the Z-isomerization.

Extraction of fucoxanthin from raw macroalgae excluding drying and cell wall disruption by liquefied dimethyl ether.

Macroalgae are one of potential sources for carotenoids, such as fucoxanthin, which are consumed by humans and animals. This carotenoid has been applied in both the pharmaceutical and food industries. In this study, extraction of fucoxanthin from wet brown seaweed Undaria pinnatifida (water content was 93.2%) was carried out with a simple method using liquefied dimethyl ether (DME) as an extractant in semi-continuous flow-type system. The extraction temperature and absolute pressure were 25 °C and 0.59 MPa, respectively. The liquefied DME was passed through the extractor that filled by U. pinnatifida at different time intervals. The time of experiment was only 43 min. The amount of fucoxanthin could approach to 390 µg/g dry of wet U. pinnatifida when the amount of DME used was 286 g. Compared with ethanol Soxhlet and supercritical CO2 extraction, which includes drying and cell disruption, the result was quite high. Thus, DME extraction process appears to be a good method for fucoxanthin recovery from U. pinnatifida with improved yields.

Intranasal administration of ceramide liposome suppresses allergic rhinitis by targeting CD300f in murine models.

Allergic rhinitis (AR) is caused by type I hypersensitivity reaction in the nasal tissues. The interaction between CD300f and its ligand ceramide suppresses immunoglobulin E (IgE)-mediated mast cell activation. However, whether CD300f inhibits the development of allergic rhinitis (AR) remains elusive. We aimed to investigate the roles of CD300f in the development of AR and the effectiveness of intranasal administration of ceramide liposomes on AR in murine models. We used ragweed pollen-induced AR models in mice. Notably, CD300f deficiency did not significantly influence the ragweed-specific IgE production, but increased the frequency of mast cell-dependent sneezing as well as the numbers of degranulated mast cells and eosinophils in the nasal tissues in our models. Similar results were also obtained for MCPT5-exprssing mast cell-specific loss of CD300f. Importantly, intranasal administration of ceramide liposomes reduced the frequency of sneezing as well as the numbers of degranulated mast cells and eosinophils in the nasal tissues in AR models. Thus, CD300f-ceramide interaction, predominantly in mast cells, alleviates the symptoms and progression of AR. Therefore, intranasal administration of ceramide liposomes may be a promising therapeutic approach against AR by targeting CD300f.

Surfactant-Free Decellularization of Porcine Auricular Cartilage Using Liquefied Dimethyl Ether and DNase.

The most common decellularization method involves lipid removal using surfactant sodium dodecyl sulfate (SDS) and DNA fragmentation using DNase, and is associated with residual SDS. We previously proposed a decellularization method for the porcine aorta and ostrich carotid artery using liquefied dimethyl ether (DME), which is free from the concerns associated with SDS residues, instead of SDS. In this study, the DME + DNase method was tested on crushed porcine auricular cartilage tissues. Unlike with the porcine aorta and the ostrich carotid artery, it is important to degas the porcine auricular cartilage using an aspirator before DNA fragmentation. Although approximately 90% of the lipids were removed using this method, approximately 2/3 of the water was removed, resulting in a temporary Schiff base reaction. The amount of residual DNA in the tissue was approximately 27 ng/mg dry weight, which is lower than the regulatory value of 50 ng/mg dry weight. Hematoxylin and eosin staining confirmed that cell nuclei were removed from the tissue. Residual DNA fragment length assessment by electrophoresis confirmed that the residual DNA was fragmented to less than 100 bp, which was lower than the regulatory limit of 200 bp. By contrast, in the uncrushed sample, only the surface was decellularized. Thus, although limited to a sample size of approximately 1 mm, liquefied DME can be used to decellularize porcine auricular cartilage. Thus, liquefied DME, with its low persistence and high lipid removal capacity, is an effective alternative to SDS.

Isothiocyanate-functionalized silica as an efficient heterogeneous catalyst for carotenoid isomerization.

Daily consumption of carotenoids is associated with multiple health benefits, but their bioavailability is generally extremely low. In this context, the Z-isomerization is receiving attention as a method for increasing carotenoid bioavailability because this approach is superior to conventional physical approaches. Here we investigated the feasibility of using isothiocyanate-functionalized silica (Si-NCS) as a heterogeneous catalyst for carotenoid isomerization. We found that this catalyst promoted Z-isomerization of (all-E)-carotenoids with high efficiency, e.g., when lycopene and astaxanthin solutions were incubated at 50 °C with 10 mg/mL Si-NCS, their total Z-isomer ratios increased by approximately 80 and 50 %, respectively. Furthermore, the Z-isomerization was successfully performed continuously by introducing carotenoid solution into a column packed with Si-NCS. Materials rich in carotenoid Z-isomers have not been used in practical applications due to high production cost and quality limitations (e.g., low Z-isomer ratio). The use of Si-NCS has sufficient potential to solve both these issues.

Gas/Liquid Pulsed Discharge Plasma in a Slug Flow Reactor under Pressurized Argon for Dye Decomposition.

Pulsed discharge plasma produced in a gas/liquid environment has attracted much attention because of its low energy requirement and the generation of various radical species with high reactivity. In our previous work, a slug flow system was developed to produce gas/liquid plasma under atmospheric pressure, generating continuous bubbles and stable gas-liquid interfaces. Currently, meaningful results have also been obtained in the field of plasma under high-pressure conditions. Therefore, in this study, a slug flow system using gas/liquid discharge plasma was implemented under pressurized argon. The system pressure was controlled from 0.1 (atmospheric pressure) to 0.4 MPa, and the effect of pressure on the system was investigated. This system was also applied to the decomposition of methylene blue. The chemical reactivity was studied, and the energy of the system was calculated. The results showed that as the system pressure increased, the decomposition rate of methylene blue decreased, while the concentration of the total oxidation species increased. This can be explained by a decrease in the energy available for methylene blue decomposition owing to the steady input energy and increasing energy loss.

High-temperature Supercritical CO2 Extraction of Lycopene from Tomato Powder for Enhancing Z-Isomerization and Recovery of Lycopene.

This study aimed to investigate the effect of extraction conditions (temperature, pressure, and entrainer content) on the total Z-isomer ratio and recovery of lycopene in the extracts obtained after supercritical CO2 (SC-CO2) extraction of lycopene from tomato powder, with a particular focus on high-temperature conditions (≥ 80°C). The results showed that high-temperature SC-CO2 extraction promoted the thermal isomerization of lycopene in a temperature-dependent manner up to 120℃. For example, when lycopene extraction was carried out at 80, 100, 120, and 140°C and a pressure of 30 MPa with an entrainer, ethanol, for 180 min, the total Z-isomer ratios obtained were 25.0, 57.2, 67.2, and 67.0%, respectively. The entrainer content also affected the Z-isomer ratio of lycopene, but the pressure had little effect. Interestingly, when SC-CO2 extraction was performed under high-temperature conditions (≥ 100°C), the extraction efficiency of lycopene was dramatically improved, e.g., when lycopene was extracted at 80, 100, 120, and 140°C under the same other conditions as above, the recovery rates of lycopene were 4.6, 28.5, 79.9, 84.8%, respectively. In general, SC-CO2 extraction of fat-soluble components is performed at temperatures in the range of 40-80°C because the SC-CO2 density decreases with increasing temperature, and thus, their solubility (extraction efficiency) decreases. However, our results showed that the lycopene recovery increased in a temperature-dependent manner, which might be due to the solubility enhancement associated with thermal Z-isomerization of lycopene (i.e., lycopene Z-isomers have greater solubility than the naturally occurring all-E-isomer). The high-temperature SC-CO2 extraction of lycopene from tomato materials not only enhances the Z-isomer ratio of lycopene in the resulting extracts but also improves lycopene recovery. This new finding will greatly contribute to the value addition and cost reduction of natural lycopene sources obtained by SC-CO2 extraction.

Reduced-Pressure Process for Fabricating Tea Tree Oil-Polyvinylpyrrolidone Electrospun Fibers.

Electrospun fibers containing tea tree oil (TTO) can be explored for practical applications due to the antimicrobial and anti-inflammatory activities of TTO. Considering that there are potentially toxic components in TTO, it is necessary to eliminate or reduce its content in the preparation process of TTO-doped electrospun fibers. In this work, electrospun TTO-PVP (polyvinylpyrrolidone) fibers containing an 18.18 wt.% decreased content of 1,8-Cineole were successfully fabricated by intense evaporation of a self-made reduced-pressure electrospinning (RP-ES) setup (as low as 94.4 kPa). In addition, such intense evaporation led to a morphology change, where a typical average fiber diameter increased from 0.831 to 1.148 µm, fewer and smaller beads in fibers, along with a rougher and grooves fiber surface. These morphology changes allowed Terpinen-4-ol to remain in the fiber for a more extended period. In addition, RP-ES proved the possibility for intense evaporation and continuous vapor removal by continuously environmental vacuum pumping of electrospinning.

Tensile Strength of Porcine Aorta Decellularized with Liquefied Dimethyl Ether and DNase.

In a previous report, we proposed a method for decellularizing porcine aortas by removing lipids from the aortas using liquefied dimethyl ether (DME) instead of the conventional sodium dodecyl sulfate (SDS). This is followed by DNA fragmentation with DNase. In the current work, the physical properties of porcine aortas decellularized using the DME method are evaluated by tensile strength tests. Conventional SDS decellularized aortas are typically swollen, rupture very easily, and have poor elasticity. By contrast, DME-treated samples are found to be less elastic. However, the maximum stress required for rupture is greater than that for the original aorta. These results indicate that decellularization with DME and DNase increases the maximum stress that can be withstood. Reduction of elasticity may derive from the appearance of temporary C=N bonds due to Schiff-base reactions that occur during the lipid removal process by liquefied DME, and methods to avoid this are desirable.

Extraction of Fucoxanthin Isomers from the Edible Brown Seaweed Undaria pinnatifida Using Supercritical CO2: Effects of Extraction Conditions on Isomerization and Recovery of Fucoxanthin.

Fucoxanthin, a characteristic carotenoid found in brown seaweeds, has been reported to exert beneficial biological activities, including antiobesity and anticancer activities Moreover, the Z-isomers of this compound potentially have greater bioavailability and biological activities than the naturally predominant all-E-isomer. Therefore, the consumption of Z-isomer-rich fucoxanthin through daily meals and dietary supplements may have beneficial effects. In this study, we aimed to investigate the effects of different extraction conditions on the Z-isomer ratio and recovery of fucoxanthin obtained from Undaria pinnatifida using supercritical CO2 (SC-CO2), particularly focusing on the high-temperature conditions that enhance thermal Z-isomerization. High-temperature SC-CO2 extraction at ≥ 120°C was found to enhance the thermal isomerization of fucoxanthin. For example, when the extraction was performed at 40, 80, 120, and 160°C and 30 MPa for 30 min with a co-solvent (ethanol), the total Z-isomer ratios were 11.7, 11.5, 18.7, and 26.5%, respectively. Furthermore, the high-temperature extraction significantly improved fucoxanthin recovery under high-pressure (≥ 30 MPa) conditions in the presence of the co-solvent. For example, when fucoxanthin was extracted at 40, 80, 120, and 160°C under the same conditions as above, the recoveries were 17.5, 20.6, 30.7, and 29.5%, respectively. Hence, the high-temperature SC-CO2 extraction of fucoxanthin from U. pinnatifida would not only enhance health benefits of fucoxanthin via the Z-isomerization but also improve the productivity. Moreover, the use of non-toxic CO2 and a low-toxicity organic solvent (ethanol) ensures that the final fucoxanthin product is safe for consumption. The Z-isomer-rich fucoxanthin obtained using this method is accordingly considered to have potential for use as a dietary supplement.

Effect of Roasting Degree on Major Coffee Compounds: A Comparative Study between Coffee Beans with and without Supercritical CO2 Decaffeination Treatment.

Coffee is a beverage that is consumed worldwide, and the demand for decaffeinated coffee has increased in recent years. This study aimed to investigate the effect of roasting conditions on the concentration of physiologically active compounds in coffee beans with and without supercritical CO2 decaffeination treatment. Decaffeination treatment markedly reduced caffeine concentration and slightly reduced trigonelline concentration in the coffee beans, whereas the concentrations of chlorogenic acids (chlorogenic acid, cryptochlorogenic acid, and neochlorogenic acid) were largely unchanged. Roasting was performed using a hot-air coffee roaster machine and the coffee beans were treated at different peak temperatures (125-250℃), different hold times at the peak temperature (120-240 s), and different temperature increase times to reach the peak temperature (60-180 s). Roasting conditions such as long hold and long temperature rise times at high temperatures (≥ 225℃) significantly degraded coffee compounds except for caffeine, with similar degradation rates between non-decaffeinated and decaffeinated coffee beans. In contrast, the L* value of decaffeinated coffee decreased with less thermal history compared to that of non-decaffeinated coffee. This allowed for the complete roasting of decaffeinated coffee with a lower thermal history compared to those of non-decaffeinated counterparts, suppressing the degradation of several coffee compounds. For example, comparing the similar L* values between coffee beans with and without decaffeination treatment, it was found that the former tended to contain more chlorogenic acid. Generally, decaffeination results in the loss of physiologically active compounds along with caffeine, which is a major concern. However, this study showed that appropriate control of decaffeination and roasting conditions can limit the degradation of several valuable coffee compounds, such as trigonelline and chlorogenic acid.

Extraction of Functional Components from Freeze-Dried Angelica furcijuga Leaves Using Supercritical Carbon Dioxide.

Angelica furcijuga (A. furcijuga), as a material for traditional Chinese medicine, has been widely used in Asian countries, such as China, Korea, and Japan, for several centuries owing to its therapeutic effects. In this study, A. furcijuga leaves were used as starting materials to extract functional substances using supercritical carbon dioxide (SC-CO2) at pressure and temperature ranges of 20-40 MPa and 40-80 °C, respectively. The extraction process was performed in a semibatch-type system with extraction times of 15-120 min. The high-performance liquid chromatography analysis indicated that kaempferol, ferulic acid, ligustilide, and butylidenephthalide as selected functional substances were successfully extracted under these operating conditions. An operating pressure of 30 MPa with an extraction time of 60 min seems to be an appropriate pressure to extract functional components from A. furcijuga leaves. The Hansen solubility parameter values and statistical analysis showed that SC-CO2 with 10% ethanol addition is a feasible tool to isolate these selected functional substances from the A. furcijuga matrix.

Enhancement of Curcuma xanthorrhiza Roxb Phytochemical Dissolution via Micronization Using a Supercritical Antisolvent Technique.

Fine particles comprising Curcuma xanthorrhiza Roxb (C. xanthorrhiza) rhizome extract were successfully generated using a supercritical carbon dioxide (SCCO2) antisolvent technique. The SCCO2 antisolvent process was performed at 40 °C with 8-16 MPa operating pressures. The CO2 and feed solution flow rates were 15 and 0.25 mL min-1, respectively. The mixture of C. xanthorrhiza rhizome extract and a polyvinylpyrrolidone (PVP) polymer in acetone-ethanol was used as a feed solution. The collected particle products seemed to possess spherical and spherical-like morphologies with a diameter of less than 500 nm. The infrared spectroscopy analysis showed that the structural properties of C. xanthorrhiza rhizome extract did not change after treatment with the SCCO2 antisolvent. Furthermore, the addition of the PVP polymer in the C. xanthorrhiza rhizome extract particle products may improve their dissolution significantly in an aqueous solution medium.

Extraction of Phytochemicals from Maypole Apple by Subcritical Water.

The Maypole apple is a new, promising species of small apples with a prominent flavor and deep red flesh and peel. This study divided Maypole apples into outer flesh, inner flesh, and peel, and used subcritical water at 100-175 °C for 10-30 min to extract various phytochemicals (procyanidin B2 (PB2), 5-caffeoylquinic acid (5CQA), and epicatechin). The obtained Maypole apple extracts and extraction residues in this work were analyzed using a SEM, HPLC, FT-IR, and UV-Vis spectrophotometer. Under different subcritical water extraction conditions, this work found the highest extraction rate: to be PB2 from the peel (4.167 mg/mL), 5CQA (2.296 mg/mL) and epicatechin (1.044 mg/mL) from the inner flesh. In addition, this work regressed the quadratic equations of the specific yield through ANOVA and found that temperature is a more significant affecting factor than extraction time. This aspect of the study suggests that phytochemicals could be obtained from the Maypole apple using the new extraction method of subcritical water.

Curcumin-Loaded Liposome Preparation in Ultrasound Environment under Pressurized Carbon Dioxide.

Curcumin-loaded liposomes were prepared using a supercritical carbon dioxide (SCCO2)−ultrasound environment system. The experiments were performed at temperatures of 40−70 °C and pressures of 10−25 MPa in a batch system with ultrasonication for 60 min. Transmission electron microscopy (TEM) images revealed liposome products with spherical morphologies and diameters of <100 nm. Dynamic light scattering (DLS) analysis indicated that the curcumin-loaded liposome nanosuspension exhibited good stability. Changing the operating conditions influenced the amount of liposome-encapsulated curcumin; as the operating temperature or pressure increased, the diameter of the liposome products and the amount of liposome-encapsulated curcumin increased and decreased, respectively. Herein, we described an innovative and practical organic-solvent-free method for generating liposomes from phospholipids.

PVP/Highly Dispersed AgNPs Nanofibers Using Ultrasonic-Assisted Electrospinning.

Silver nanoparticles (AgNPs) are novel materials with antibacterial, antifungal, and antiviral activities over a wide range. This study aimed to prepare polyvinylpyrrolidone (PVP) electrospinning composites with uniformly distributed AgNPs. In this study, starch-capped ~2 nm primary AgNPs were first synthesized using Atmospheric pressure Pulsed Discharge Plasma (APDP) at AC 10 kV and 10 kHz. Then, 0.6 wt.% AgNPs were mixed into a 10 wt.% PVP ethanol-based polymer solution and coiled through an Ultrasonic-assisted Electrospinning device (US-ES) with a 50 W and 50 kHz ultrasonic generator. At 12 kV and a distance of 10 cm, this work successfully fabricated AgNPs-PVP electrospun fibers. The electrospun products were characterized using Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), High-Resolution TEM (HR-TEM), Fourier Transform Infrared Spectroscopy (FT-IR), X-ray Diffraction (XRD), Thermogravimetric (TG), and X-ray Photoelectron Spectroscopy (XPS) methods.

Oil removal from spent bleaching earth of vegetable oil refinery plant using supercritical carbon dioxide.

The oil in the spent bleaching earth (SBE) matrix was successfully removed by applying the supercritical carbon dioxide (SCCO2) extraction technique in a semi-continuous flow-type system. The SCCO2 extraction process was conducted at 40-80 °C and 20-30 MPa with extraction time of ∼180 min. The color of SBE matrix changes from the dark to dark-pale color after the SCCO2 extraction treatment exhibiting the substances including oil in the SBE matrix were successfully removed. The extracted oil yield was around 95% when the SCCO2 extraction process was performed at 40 °C and 30 MPa with 10% ethanol addition as a co-solvent. The GC analysis showed that the prominent fatty acid constituents in the extracted oil are palmitic and oleic acids, furthermore it can be fed as a feedstock to produce biodiesel fuel. Next, it can be proposed that SCCO2 extraction system is a viable way to extract oil from the SBE matrix.