Evaluating the effects of hypoxic storage on platelet function and health using a novel storage system.

BACKGROUND: Thousands of units of whole blood (WB) and blood components are transfused daily to treat trauma patients. Improved methods for blood storage are critical to support trauma-related care. The Hemanext ONE® system offers a unique method for hypoxic storage of WB, with successfully demonstrated storage of clinically viable RBCs. This work evaluated the system for the storage of WB, focusing on platelet health and function. STUDY DESIGN AND METHODS: WB was collected from healthy donors and processed through the Hemanext ONE® system. Hemoglobin oxygen saturation (HbSO2) levels of WB were depleted to 10%, 20%, or 30% of total HbSO2 and then stored in PVC bags sealed in oxygen-impermeable bags (except for normoxic control) with samples collected on days 1, 7, and 14 post-processing. Flow cytometry assessed the activation and apoptosis of platelets. Clot dynamics were assessed based on aggregometry and thromboelastography assays, as well as thrombin generation using a calibrated-automated thrombogram method. RESULTS: Hypoxic storage conditions were maintained throughout the storage period. Hypoxia triggered increased lactate production, but pH changes were negligible compared to normoxic control. Storage at 10% HbSO2 had a significant impact on platelet function, resulting in increased activation and reduced clot formation and aggregation. These effects were less significant at 20% and 30% HbSO2. DISCUSSION: This study indicates that platelets are sensitive to hypoxic storage and suffer significant metabolic and functional deterioration when stored at or below 10% HbSO2.

Food restriction during organogenesis in rabbits: effects on reproduction and the offspring.

To assess the effects of markedly restricted food intake versus ad libitum feeding or a slightly restricted feeding regimen during the period of organogenesis we fed groups of 16-18 pregnant rabbits Purina Certified High Fiber Chow ad libitum, 150 g/day, 75 g/day, or 15 g/day on Gestation Days 6 to 18 inclusive. Prior to and after organogenesis the animals were provided food ad libitum (ad lib). Clinical observations, body weights, and food and water consumption were recorded daily. On Gestation Day 30 each doe was euthanatized and necropsied, and maternal and fetal data were collected. Each fetus was examined for external, visceral, and skeletal variations and malformations. Ossification parameters were also evaluated. Statistical analyses were conducted in two ways, first comparing the restricted groups to the ad lib group and second comparing the 15 and 75 g/day groups to the 150 g/day group. During Days 6-18, the 15 and 75 g/day groups had significantly decreased weight gain (actual weight loss), compared to the groups fed 150 g/day or ad lib. Water consumption was also significantly decreased in the 15 g/day group during this period, compared to the ad lib group. When food was provided ad lib on Days 19-30 to the restricted groups, weight gain was significantly higher in the 15 and 75 g/day groups than the group previously given 150 g/day and the ad lib group. There were no differences in water consumption during that period. Abortion occurred in three 15 g/day animals.(ABSTRACT TRUNCATED AT 250 WORDS)