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1.
Macromol Rapid Commun ; 45(15): e2400158, 2024 Aug.
Article in English | MEDLINE | ID: mdl-38651593

ABSTRACT

Carbon-chain dendritic polymers hold unique properties and promising applications. However, synthesizing carbon-chain dendrimers, beyond conjugated ones, remains a challenge. Here, the use of the iterative single unit monomer insertion technique for synthesizing 2.5 generation partial-carbon-chain dendrimers (G2.5) is described, utilizing bismaleimide as the core, a maleimide-trithiocarbonate conjugate as the branching unit, and indene as the spacer unit, following a divergent growth strategy. The optimized conditions for synthesizing the maleimide-trithiocarbonate branching unit are a bismaleimide to trithiocarbonate ratio of 5:1 and a reaction time of 30 min. The structures are verified using 1H nuclear magnetic resonance, gel permeation chromatography, and matrix-assisted laser desorption/ionization-time of flight mass spectra. A four-arm star polymer is then synthesized using the G2.5 as the core. This synthesis of a partial-carbon-chain dendrimer establishes a foundational step toward creating all-carbon-chain ones and may open new application avenues in material science.


Subject(s)
Carbon , Dendrimers , Dendrimers/chemistry , Dendrimers/chemical synthesis , Carbon/chemistry , Molecular Structure , Maleimides/chemistry , Maleimides/chemical synthesis , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization , Polymerization , Polymers/chemistry , Polymers/chemical synthesis
2.
Reprod Health ; 21(1): 86, 2024 Jun 17.
Article in English | MEDLINE | ID: mdl-38886725

ABSTRACT

BACKGROUND: To discuss the current status of reproductive concerns and its correlation with fear of recurrence and level of family support in patients of childbearing age with gynecologic malignancies. METHODS: A convenient sampling method was used to enroll 188 patients with gynecologic malignancies in Nanjing Maternity and Child Health Care Hospital, Nanjing Drum Tower Hospital, General Hospital of Ningxia Medical University, and Haian Hospital of Traditional Chinese Medicine Affiliated to Nanjing University of Chinese Medicine from September 2022 to April 2023. Patients were assessed using general information questionnaire, Reproductive Concerns After Cancer Scale (RCAC), Fear of Cancer Recurrence Inventory (FCRI) questionnaire, and Perceived Social Support-Family (PSS-FA) Scale. RESULTS: Among patients of childbearing age with gynecologic malignancies, the total RCAC score was (54.35 ± 7.52), indicating a moderate level of reproductive concerns. Patients scored (20.98 ± 4.51) on FCRI, implying a moderate level of fear of recurrence. The PSS-FA score was (9.57 ± 2.76), denoting a moderate level of family support. The total score and each dimensional score of RCAC were positively correlated with FCRI total score (P < 0.05), and negatively correlated with PSS-FA total score (P < 0.05). Fear of recurrence, family support level, number of children, educational background, treatment modality, and fertility intention were influencing factors for reproductive concerns in patients of childbearing age with gynecologic malignancies (all P < 0.05). CONCLUSION: The reproductive concerns, fear of recurrence and family support are all at moderate levels in patients of childbearing age with gynecologic malignancies, and reproductive concerns are positively correlated with fear of recurrence and negatively correlated with family support.


Subject(s)
Family Support , Fear , Genital Neoplasms, Female , Neoplasm Recurrence, Local , Adolescent , Adult , Female , Humans , Middle Aged , Young Adult , China/epidemiology , Fear/psychology , Genital Neoplasms, Female/psychology , Neoplasm Recurrence, Local/psychology , Surveys and Questionnaires
3.
J Recept Signal Transduct Res ; 41(6): 593-603, 2021 Dec.
Article in English | MEDLINE | ID: mdl-33108937

ABSTRACT

C-X-C motif chemokine ligand 14 (CXCL14) has antitumor effect. Kinase B (Akt)/mammalian target of rapamycin (mTOR) pathway is activated in various tumors. The relationship between CXCL14 and Akt/mTOR pathway in hepatocellular carcinoma (HCC) remained elusive. Therefore, this paper aimed to examine their interaction in HCC. First, CXCL14 expression was determined to be low-expressed in HCC tissues and cells (SNU-423, SNU-182, SNU-387, PLC/PRF/5, HuH7, and HCCLM3). Then, CXCL14 was overexpressed in HuH7 cells and inhibited in HCCLM3 cells to help investigate the function of CXCL14 on cell viability, growth and apoptosis. Akt activator (SC79) and inhibitor (AZD5363) were used to examine the involvement of Akt pathway in hepatocellular carcinoma. Overexpressed CXCL14 suppressed cell viability and growth, but promoted the apoptosis by upregulated Bax and cleaved(C) caspase-3, donwregulated Bcl-2 and the inhibition of Akt and mTOR phosphorylation. Meanwhile, knockdown of CXCL14 imposed an opposite effect to overexpressed CXCL14. SC79 partially mitigated the functions of overexpressed CXCL14, while AZD5363 mitigated the functions of CXCL14 knockdown. To conclude, CXCL14 inhibited growth but promoted apoptosis of HCC cells via suppressing Akt/mTOR pathway, thus, CXCL14 might be a potential target for HCC treatment in clinical practice.


Subject(s)
Biomarkers, Tumor/metabolism , Carcinoma, Hepatocellular/pathology , Chemokines, CXC/metabolism , Gene Expression Regulation, Neoplastic , Phosphatidylinositol 3-Kinases/chemistry , Proto-Oncogene Proteins c-akt/antagonists & inhibitors , TOR Serine-Threonine Kinases/antagonists & inhibitors , Apoptosis , Biomarkers, Tumor/genetics , Carcinoma, Hepatocellular/genetics , Carcinoma, Hepatocellular/metabolism , Cell Proliferation , Chemokines, CXC/genetics , Female , Humans , Liver Neoplasms/genetics , Liver Neoplasms/metabolism , Liver Neoplasms/pathology , Phosphatidylinositol 3-Kinases/genetics , Phosphatidylinositol 3-Kinases/metabolism , Phosphorylation , Proto-Oncogene Proteins c-akt/genetics , Proto-Oncogene Proteins c-akt/metabolism , TOR Serine-Threonine Kinases/genetics , TOR Serine-Threonine Kinases/metabolism , Tumor Cells, Cultured
4.
BMC Cancer ; 19(1): 1063, 2019 Nov 08.
Article in English | MEDLINE | ID: mdl-31703647

ABSTRACT

BACKGROUND: Prostate cancer is one of the most common cancers in the world. The results of treatment after hypofractionated radiotherapy only have been reported from several small randomized clinical trials. Therefore, we conducted a meta-analysis to compare clinical outcomes of hypofractionated radiotherapy versus conventional radiotherapy in the treatment of intermediate- to high-risk localized prostate cancer. METHODS: Relevant studies were identified through searching related databases till August 2018. Hazard ratio (HR) or risk ratio (RR) with its corresponding 95% confidence interval (CI) was used as pooled statistics for all analyses. RESULTS: The meta-analysis results showed that overall survival (HR = 1.12, 95% CI: 0.93-1.35, p = 0.219) and prostate cancer-specific survival (HR = 1.29, 95% CI: 0.42-3.95, p = 0.661) were similar in two groups. The pooled data showed that biochemical failure was RR = 0.90, 95% CI: 0.76-1.07, p = 0.248. The incidence of acute adverse gastrointestinal events (grade ≥ 2) was higher in the hypofractionated radiotherapy (RR = 1.70, 95% CI: 1.12-2.56, p = 0.012); conversely, for late grade ≥ 2 gastrointestinal adverse events, a significant increase in the conventional radiotherapy was found (RR = 0.75, 95% CI: 0.61-0.91, p = 0.003). Acute (RR = 1.01, 95% CI: 0.89-1.15, p = 0.894) and late (RR = 0.98, 95% CI: 0.86-1.10, p = 0.692) genitourinary adverse events (grade ≥ 2) were similar for both treatment groups. CONCLUSION: Results suggest that the efficacy and risk for adverse events are comparable for hypofractionated radiotherapy and conventional radiotherapy in the treatment of intermediate- to high-risk localized prostate cancer.


Subject(s)
Prostatic Neoplasms/mortality , Prostatic Neoplasms/radiotherapy , Radiation Dose Hypofractionation , Randomized Controlled Trials as Topic , Gastrointestinal Tract/radiation effects , Humans , Male , Prostatic Neoplasms/ethnology , Radiation Injuries/etiology , Survival Rate , Treatment Outcome , Urogenital System/radiation effects , White People
5.
Apoptosis ; 21(1): 110-9, 2016 Jan.
Article in English | MEDLINE | ID: mdl-26496775

ABSTRACT

Transarterial chemoembolization (TACE) is the standard of care for treatment of intermediate hepatocellular carcinoma (HCC), however, key molecules involved in HCC cell survival and tumor metastasis post-TACE remain unclear. CD147 is a member of the immunoglobulin superfamily that is overexpressed on the surface of HCC cells and is associated with malignant potential and poor prognosis in HCC patients. In this study, using an Earle's Balanced Salt Solution medium culture model that mimics nutrient deprivation induced by TACE, we investigated the regulation of CD147 expression on HCC cells under starvation conditions and its functional effects on HCC cell death. During early stages of starvation, the expression of CD147 was considerably upregulated in SMMC7721, HepG2 and HCC9204 hepatoma cell lines at the protein levels. Downregulation of CD147 by specific small interfering RNA (siRNA) significantly promoted starvation-induced cell death. In addition, CD147 siRNA-transfected SMMC7721 cells demonstrated significantly increased levels of both apoptosis and autophagy as compared to cells transfected with control siRNA under starvation conditions, whereas no difference was observed between the two treatment groups under normal culture conditions. Furthermore, silencing of CD147 resulted in a remarkable downregulation of phosphorylated mammalian target of rapamycin (p-mTOR) in starved SMMC7721 cells. Finally, the combined treatment of starvation and anti-CD147 monoclonal antibody exhibited a synergistic HCC cell killing effect. Our study suggests that upregulation of CD147 under starvation may reduce hepatoma cell death by modulating both apoptosis and autophagy through mTOR signaling, and that CD147 may be a novel potential molecular target to improve the efficacy of TACE.


Subject(s)
Basigin/genetics , Gene Expression Regulation, Neoplastic , Gene Silencing , TOR Serine-Threonine Kinases/genetics , Antibodies, Monoclonal/pharmacology , Apoptosis/drug effects , Autophagy/drug effects , Basigin/metabolism , Cell Line, Tumor , Culture Media/pharmacology , Hep G2 Cells , Humans , Phosphorylation/drug effects , RNA, Small Interfering/genetics , RNA, Small Interfering/metabolism , Signal Transduction , TOR Serine-Threonine Kinases/metabolism
6.
J Hazard Mater ; 480: 135883, 2024 Sep 17.
Article in English | MEDLINE | ID: mdl-39303617

ABSTRACT

Microbial remediation of heavy metal (HM)-contaminated soil is a sustainable approach; however, the impact of microbial inoculation on the internal environment of plants remains understudied. Thus, Enterobacter sp. FM-1 (Enterobacter sp.) and the hyperaccumulator Bidens pilosa L. (B. pilosa L.) were used to study these effects. Through analyses of plant physiological and biochemical characteristics, the endophytic microbial community composition, microbial co-occurrence networks and functional predictions, the potential mechanisms by which Enterobacter sp. benefits the phytoremediation of HM-contaminated soil by B. pilosa L. were elucidated. Inoculation with Enterobacter sp. promoted the growth of B. pilosa L. and influenced the endophytic microbial community diversity in B. pilosa L. Interactions among endophytes facilitated the formation of microbial networks, with endophytic fungi playing a more prominent role than endophytic bacteria as the level of HM contamination increased. Functional predictions via PICRUSt2 revealed that endophytic bacteria are involved primarily in processes related to carbohydrate metabolism, ABC transporters, and amino acid metabolism. In conclusion, this study provides evidence for the beneficial role of microbes in improving the plant endosphere environment.

7.
Se Pu ; 42(8): 805-811, 2024 Aug.
Article in Zh | MEDLINE | ID: mdl-39086250

ABSTRACT

Tobacco flavor, an important tobacco additive, is an essential raw material in cigarette production that can effectively improve the quality of tobacco products, add aroma and taste, and increase the suction flavor. The quality consistency of tobacco flavors affects the quality stability of branded cigarettes. Therefore, the quality control of tobacco flavors is a major concern for cigarette and flavor manufacturers. Physical and chemical indices, odor similarity, and sensory efficacy are employed to evaluate the quality of tobacco flavors, and the analysis of chemical components in tobacco flavors is usually conducted using gas chromatography (GC) and high performance liquid chromatography (HPLC). However, because the composition of tobacco flavors is complex, their quality cannot be fully reflected using a single component or combination of components. Therefore, establishing an objective analytical method for the quality control of tobacco flavors is of extreme importance. Chromatographic fingerprint analysis is routinely used for the discriminative analysis of tobacco flavors. Chromatographic fingerprints refer to the general characteristics of the concentration profiles of different chemical compounds. In the daily procurement process, fingerprints established by GC and HPLC are effective for the evaluation and identification of tobacco flavors. However, given continuous improvements in aroma-imitation technology, some flavors with high similarity cannot be directly distinguished using existing methods. In this study, a method for the determination of organic acids and inorganic anions in tobacco flavors based on ion chromatography (IC) was developed to ensure the quality consistency of tobacco flavors. A 1.0 g sample of tobacco flavors and 10 mL of deionized water were mixed and vibrated for 30 min. The aqueous sample solution was passed through a 0.45 µm membrane filter and RP pretreatment column in succession to eliminate interferences and then subjected to IC. Standard solutions containing nine organic acids and seven inorganic anions were used to identify the anions in the tobacco flavors, and satisfactory reproducibility was obtained. The relative standard deviations (RSDs) for retention times and peak areas were <0.71% and <6.02%, respectively. The chromatographic fingerprints of four types of tobacco flavors (samples A-D) from five different batches were obtained. Nine tobacco flavor samples from different manufacturers (samples AY1-AY3, BY1-BY2, CY1-CY2, DY1-DY2) were also analyzed to obtain their chromatographic fingerprints. Hierarchical cluster and similarity analyses were used to evaluate the quality of tobacco flavors from different manufacturers. Hierarchical clustering refers to the process of subdividing a group of samples into clusters that exhibit a high degree of intracluster similarity and intercluster dissimilarity. The dendrograms obtained using SPSS 12.0 indicated good quality consistency among the samples in different batches. Samples AY3, BY2, CY2, and DY1 clustered with the batches of standard tobacco flavors. Therefore, hierarchical cluster analysis can effectively distinguish the quality of products from different manufacturers. The Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine (version 2.0) was used to evaluate the similarity between the standard tobacco flavors and products from different manufacturers. Among the samples analyzed, samples AY3, BY2, CY2, and DY1 showed the highest similarity values (>97.7%), which was consistent with the results of the hierarchical cluster analysis. This finding indicates that IC combined with chromatographic fingerprint analysis could accurately determine the quality of tobacco flavors. GC combined with ultrasonic-assisted liquid-liquid extraction was also used to analyze the tobacco flavors and verify the accuracy of the proposed method. Compared with GC coupled with ultrasonic-assisted liquid-liquid extraction, IC demonstrated more significant quality differences among certain tobacco flavors.


Subject(s)
Nicotiana , Quality Control , Nicotiana/chemistry , Flavoring Agents/analysis , Tobacco Products/analysis , Chromatography, High Pressure Liquid/methods , Chromatography, Gas/methods , Chromatography, Ion Exchange/methods
8.
Eur Arch Otorhinolaryngol ; 270(5): 1597-605, 2013 May.
Article in English | MEDLINE | ID: mdl-22907029

ABSTRACT

Ototoxicity is a common side effect of cisplatin chemotherapy. This study was undertaken to determine the potential protective effects of a systemic administration of dexamethasone against cisplatin-induced ototoxicity. A prospective controlled trial conducted in an animal model. The setting was Animal care research facilities of the Montreal Children's Hospital Research Institute. An experimental guinea pig model was used. The animals were divided as follows: group 1 (n = 10): 12 mg/kg intraperitoneal (IP) cisplatin, group 2 (n = 14): 15 mg/kg/day dexamethasone IP for 2 days followed by cisplatin 12 mg/kg IP, group 3 (n = 14): 10 mg/kg/day dexamethasone IP for 2 days, on day 3, they received cisplatin 12 mg/kg IP followed by 20 mg/kg/day dexamethasone for 2 days and group 4 (n = 5): 10 ml of saline IP twice a day for 3 days. Auditory brainstem response (ABR) threshold shifts were measured at four frequencies (8, 16, 20 and 25 kHz) for groups 1, 2 and 3. Histological changes in the organ of Corti, the stria vascularis, the spiral ligament and the spiral ganglion neurons as well as scanning electron microscopy for outer hair cells were completed. Immunohistochemistry for tumour necrosis factor-alpha (TNF-α) was performed. ABR threshold shifts were similar in all groups. Histological and scanning electron findings demonstrate that dexamethasone has greater protective effect on the stria vascularis. Systemic dexamethasone administration in a guinea pig model did not provide significant protection against cisplatin-induced ototoxicity. Dexamethasone may be useful in future applications as a complementary treatment.


Subject(s)
Anti-Inflammatory Agents/pharmacology , Antineoplastic Agents/adverse effects , Cisplatin/adverse effects , Cochlea/drug effects , Dexamethasone/pharmacology , Evoked Potentials, Auditory, Brain Stem/drug effects , Hearing Loss, Sensorineural/chemically induced , Animals , Cochlea/pathology , Cochlea/ultrastructure , Female , Guinea Pigs , Hearing Loss, Sensorineural/prevention & control , Microscopy, Electron, Scanning , Organ of Corti/drug effects , Organ of Corti/pathology , Organ of Corti/ultrastructure , Prospective Studies , Spiral Ganglion/drug effects , Spiral Ganglion/pathology , Spiral Ganglion/ultrastructure , Spiral Ligament of Cochlea/drug effects , Spiral Ligament of Cochlea/pathology , Spiral Ligament of Cochlea/ultrastructure , Stria Vascularis/drug effects , Stria Vascularis/pathology , Stria Vascularis/ultrastructure , Tumor Necrosis Factor-alpha/metabolism
9.
Environ Sci Pollut Res Int ; 30(56): 118897-118915, 2023 Dec.
Article in English | MEDLINE | ID: mdl-37919506

ABSTRACT

Green credit is an important topic in the study of green finance system, but it has not been combined with China's carbon emission reduction effect and industrial carbon emissions. This study takes different industries in China as research objects to explore the relationship between green credit and industrial carbon emissions. First, the LMDI decomposition model was used to decompose the driving factors of industrial carbon emissions, and the effects of green credit efficiency and scale on carbon emissions were obtained. Secondly, on this basis, a system dynamics model was established to predict the changing trend of carbon emissions in different industries. By setting different scenarios of green credit, the development and evolution trend of carbon emission system was simulated when parameters changed, and the Tapio decoupling model was further established to analyze the decoupling effect of green credit and carbon emissions under different scenarios. Finally, the research results show that the increase in the scale of green credit can effectively inhibit carbon emissions and has the greatest effect on carbon emissions of the secondary industry. The incentive policy of green credit can effectively encourage industrial upgrading and development. With the growth of the balance of green credit, green credit and carbon emissions gradually reach the best decoupling state. This study provides empirical evidence for the objective evaluation of the implementation effect of China's green credit policy and has important reference value for the improvement and development of future policies.


Subject(s)
Carbon Dioxide , Carbon , Carbon/analysis , Carbon Dioxide/analysis , Economic Development , China , Industry
10.
J Chromatogr A ; 1710: 464415, 2023 Nov 08.
Article in English | MEDLINE | ID: mdl-37783003

ABSTRACT

Hollow fiber-solid phase microextraction combined with micro sample collector assisted injection technique was developed for the detection of trace fatty acid methyl esters in biodiesel wastewater. Polypropylene hollow fiber was employed as extraction material to absorb fatty acid methyl esters in biodiesel wastewater. After the adsorption, hollow fiber was sleeved on the needle core of a micro sample collector and introduced directly into a GC injector for thermal desorption of the analytes. The selectivity of polypropylene hollow fiber on fatty acid methyl esters was investigated by extracting common pollutants in wastewater. Under the optimal conditions, the enrichment factors of polypropylene hollow fiber for methyl palmitate, methyl linoleate, methyl oleate, and methyl stearate were tested as high as 471, 287, 527, and 801, respectively. The quantitative method was validated and the linearity was satisfactory over a concentration range of 10-2000 µg/L with the correlation coefficients more than 0.9990 for 4 fatty acid methyl esters. The limits of detection and quantification were 0.04-0.40 µg/L and 10.0 µg/L, respectively. The recoveries were in the range of 92.0-116.7% by analyzing actual spiked samples. The results showed that the established method was suitable for the analysis of trace fatty acid methyl esters in water samples, with simple operation, low cost and environmental friendliness.


Subject(s)
Solid Phase Microextraction , Wastewater , Solid Phase Microextraction/methods , Biofuels , Polypropylenes , Adsorption
11.
Ann Transl Med ; 10(24): 1328, 2022 Dec.
Article in English | MEDLINE | ID: mdl-36660612

ABSTRACT

Background: Ginsenoside Rg1, a major bioactive ingredient of Panax notoginseng, has been shown to reduce gut inflammation and ameliorate experimental colitis in mice. However, it is not yet known whether it affects the intestinal barrier injury of colitis. Methods: This study explored the effect of ginsenoside Rg1 on intestinal barrier injury in dextran sulfate sodium (DSS)-induced colitis mice through an ultrastructure observation of the colonic mucosa and analysis of the expression of colonic cytoplasmatic zonula occludens-1 (ZO-1) protein. Results: Treatment with ginsenoside Rg1, especially high-dose use, significantly ameliorated colonic histopathologic features and the severity of the colitis and reduced colonic tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ) levels and increase IL-4 levels in a mouse model of DSS-induced colitis. Its observed efficacy was comparable to that of 5-Aminosalicylic acid (5-ASA), a first-line therapeutic agent for ulcerative colitis. Notably, ginsenoside Rg1 administration was shown to up-regulate the expression of colonic ZO-1 protein, and it repaired the intestinal barrier structure in DSS-induced colitis mice. Conclusions: Taken together, our findings demonstrated that ginsenoside Rg1 treatment can significantly ameliorate the severity of DSS-induced colitis in mice, which involves intestinal barrier structure remodeling through lowering the levels of the colonic pro-inflammatory cytokines TNF-α and IFN-γ and increasing the anti-inflammatory cytokine IL-4. These results suggest the potential therapeutic use of ginsenoside Rg1 as a promising approach for the treatment of inflammatory bowel disease (IBD).

12.
J Urol ; 186(4 Suppl): 1625-9, 2011 Oct.
Article in English | MEDLINE | ID: mdl-21862070

ABSTRACT

PURPOSE: We objectively quantified the gain in urethral diameter and the effect of stenting after tubularized incised plate urethroplasty in a rabbit hypospadias model. MATERIALS AND METHODS: We created a hypospadias model in 12 New Zealand white male rabbits by excising the ventral urethra. A 3 cm tattoo line was made longitudinally in the dorsal urethral plate midline. Two weeks later a 2 cm relaxing incision was made in the middle part of the tattooed line. The stretched incision width between the tattooed edges was measured, followed by urethral plate tubularization. Six rabbits were stented and 6 were nonstented. Two weeks later the animals were sacrificed and the distance separating the tattoo was measured at the midpoint of the tattooed line. Transverse sections at this point were examined histologically. RESULTS: All animals survived the procedures. Stents were removed at 7 days in 4 rabbits and fell out in 2 at 4 and 2 days, respectively. The mean ± SD incision width of 5.5 ± 1.6 mm (range 3 to 8) at tubularization became 2 ± 0.5 mm (range 1 to 3) after healing (p <0.002). Mean width of the healed incision was 1.7 ± 0.4 (range 1 to 2) vs 2.3 ± 0.5 mm (range 1.5 to 3) in the nonstented and stented groups, respectively (p <0.06). Rabbits with a stenting duration of less than 7 days were excluded from the last analysis. Histologically all incisions healed completely with an intact epithelium. CONCLUSIONS: The initial width of the midline relaxing incision significantly decreased after complete epithelialization. The average gain in urethral width was only 2 mm. Stenting appeared to increase the width of the healed incisions but not in a statistically significant manner.


Subject(s)
Hypospadias/surgery , Plastic Surgery Procedures/methods , Stents , Urethra/surgery , Urologic Surgical Procedures, Male/methods , Animals , Disease Models, Animal , Follow-Up Studies , Hypospadias/pathology , Male , Prosthesis Design , Rabbits , Urethra/pathology , Wound Healing
13.
Chin Med Sci J ; 26(1): 43-8, 2011 Mar.
Article in English | MEDLINE | ID: mdl-21496422

ABSTRACT

OBJECTIVE: To observe the influence of Shenqing Recipe (SQR), a kind of Traditional Chinese Medicine, on the morphology and quantity of colonic interstitial cells of Cajal (ICC) in trinitrobenzene sulfonic acid (TNBS)-induced rat colitis, and to investigate the possible mechanism of SQR in regulating intestinal dynamics. METHODS: Sixty rats were randomly divided into normal control, model 1, model 2, mesalazine, and high-dose, and low-dose SQR groups with 10 rats in each group. TNBS (10 mg) dissolved in 50% ethanol was instilled into the lumen of the rat colon of the latter five groups to induce colitis. On the 4th day after administration of TNBS, each treatment group was administered one of the following formulations by enteroclysis gavage once a day for 7 days: 600 mg•kg⁻¹â€¢d⁻¹ mesalazine, 2.4 g•kg⁻¹â€¢d⁻¹ SQR, and 1.2 g•kg⁻¹â€¢d⁻¹ SQR. Model 2 rats received normal saline solution. After 7 days colonic samples were collected. While the colonic samples of model 1 group were collected on the 3rd day after TNBS administered. Ultrastructure of ICC in the damaged colonic tissues was observed with transmission electron microscope. Expression of c-kit protein in colonic tissue was determined by immunohistochemical staining and Western blot. RESULTS: The ultrastructure of colonic ICC in the rat model of TNBS-induced colitis showed a severe injury, and administration of SQR or mesalazine reduced the severity of injury. Similarly, the expression of c-kit protein of TNBS-induced colitis rat model was significantly decreased compared with the normal control group (P < 0.05). Treatment with SQR or mesalazine significantly increased the expression of c-kit protein compared with the administration of control formulations (P < 0.05), especially the high-dose SQR group. CONCLUSION: SQR could alleviate and repair the injured ICC, and improve its quantity, which might be involved in regulating intestinal motility.


Subject(s)
Colitis/chemically induced , Colitis/drug therapy , Colon/cytology , Drugs, Chinese Herbal/pharmacology , Drugs, Chinese Herbal/therapeutic use , Interstitial Cells of Cajal/drug effects , Trinitrobenzenesulfonic Acid/adverse effects , Animals , Colitis/pathology , Colon/metabolism , Interstitial Cells of Cajal/pathology , Interstitial Cells of Cajal/ultrastructure , Male , Medicine, Chinese Traditional , Mesalamine/therapeutic use , Peroxidase/metabolism , Proto-Oncogene Proteins c-kit/metabolism , Random Allocation , Rats , Rats, Sprague-Dawley
14.
Oncol Rep ; 46(3)2021 Sep.
Article in English | MEDLINE | ID: mdl-34328200

ABSTRACT

Tumor microenvironment (TME) can serve as the 'soil' for the growth and survival of tumor cells and function synergically with tumor cells to mediate tumor progression and therapeutic resistance. Reactive oxygen species (ROS) is somewhat of a double­edged sword for tumors. Accumulating evidence has reported that regulating ROS levels can serve an anti­tumor role in the TME, including the promotion of cancer cell apoptosis, inhibition of angiogenesis, preventing immune escape, manipulating tumor metabolic reorganization and improving drug resistance. In the present review, the potential role of ROS in anti­tumor therapy was summarized, including the possibility of directly or indirectly targeting the TME.


Subject(s)
Neoplasms/metabolism , Reactive Oxygen Species/metabolism , Tumor Microenvironment , Humans
15.
J Pharm Biomed Anal ; 201: 114101, 2021 Jul 15.
Article in English | MEDLINE | ID: mdl-33984829

ABSTRACT

Lysophosphatidylcholine (LPC) was commonly known as a class of significant differential metabolites of high relevance with many diseases including psoriasis, of which the accurate determination is of great importance to diagnosis or prediction to many diseases. However, it is challenging and complicated because of the enormous biological sample complexity and impurities interference. In this study, we synthesized a magnetic nanocomposite MG@PD@TiO2 and took advantage of the interactions of Lewis acid-base between the phosphate groups in LPCs and Ti ions on MG@PD@TiO2 nanomaterials for selective separation and enrichment of LPCs from complex biological matrix. The solid-phase extraction sample pretreatment process by means of MG@PD@TiO2 nanomaterials coupled with LC-MS/MS method was then applied to actual determination of six typical LPCs (LPC 10:0, 14:0, 16:0, 18:0, 18:1, 22:0) in human plasma. The extraction conditions were scientifically optimized by single-factor test (adsorbent amount, adsorption and desorption time, elution solvent type, eluant volume). Under the optimal conditions, the detection limits (LOD, S/N = 3) and quantification limits (LOQ, S/N = 10) were 1 and 5 ng/mL for LPC 10:0 and LPC 14:0, 0.02 and 0.1 ng/mL for LPC 16:0 and LPC 18:1, 0.05 and 0.2 ng/mL LPC 18:0 and LPC 22:0, respectively. The intra- and inter-day precisions were 3.82-12.60 % (n = 6) and 3.29-13.50 % (n = 6) respectively, the recoveries were in the range of 91.92-113.69 % and the stability of the analytes in the matrix performed well with RSDs≤15.51 %. Finally, the developed method was successfully applied to the accurate determination of six LPCs biomarkers of plasma in patients with psoriasis (n = 10) and control groups (n = 10).


Subject(s)
Nanocomposites , Psoriasis , Biomarkers , Chromatography, Liquid , Humans , Limit of Detection , Lysophosphatidylcholines , Magnetic Phenomena , Psoriasis/diagnosis , Solid Phase Extraction , Tandem Mass Spectrometry , Titanium
16.
Sheng Li Xue Bao ; 62(2): 143-8, 2010 Apr 25.
Article in Zh | MEDLINE | ID: mdl-20401449

ABSTRACT

The present study aimed to investigate the change of cytochrome c in postconditioning-attenuated ischemia-reperfusion (I/R)-induced mucosal apoptosis in rat intestine compared with ischemic preconditioning (IPC). Using rat model of intestine I/R injury, male Sprague-Dawley rats weighing 220-250 g were divided into 4 groups which were Sham operation group, I/R group, IPC group and ischemic postconditioning (IPOST) group. In these groups, I/R procedure was performed by the occlusion of the superior mesenteric artery (SMA) for 45 min followed by reperfusion for 1 h. In Sham group, there was no intervention. In IPC group, SMA was occluded for 5 min and reperfused for 5 min, for two cycles, before the prolonged occlusion. In IPOST group, three cycles of 30-s reperfusion and 30-s reocclusion were preceded at the start of reperfusion. After the reperfusion, the small intestines were sampled for experimental detection. Intestinal mucosal mitochondrial membrane potential was detected by confocal laser scanning microscopy. Expressions of cytochrome c and caspase-3 proteins were detected using Western-blot method. The apoptosis of intestinal mucosal cells was determined with agarose gel electrophoresis and deoxynucleotidyl transferase mediated dUTP-biotin nick-end labeling (TUNEL) technique. Compared with I/R group, the mitochondrial membrane potentials and the expressions of cytochrome c protein were significantly increased, while the expressions of caspase-3 and the apoptotic rates were decreased in IPOST and IPC groups (P<0.05). There were no significant differences between IPOST and IPC groups (P>0.05). These data provide substantial evidence that IPOST attenuates I/R-induced mucosal apoptosis by reducing the release of cytochrome c from mitochondria in the rat small intestine.


Subject(s)
Apoptosis/physiology , Cytochromes c/metabolism , Intestines/blood supply , Ischemic Postconditioning/methods , Reperfusion Injury/prevention & control , Animals , Intestinal Mucosa/metabolism , Intestinal Mucosa/pathology , Male , Membrane Potential, Mitochondrial , Mitochondria/metabolism , Rats , Rats, Sprague-Dawley
17.
Antiviral Res ; 79(1): 12-8, 2008 Jul.
Article in English | MEDLINE | ID: mdl-18359097

ABSTRACT

In this study, we have generated and characterized an avian influenza H5N1 hemagglutinin (HA), neuraminidase (NA) and M2 ion channel pseudotyped HIV-based vector system (HaNaM-pseudotyped HIV vector). The cleavage site of the HA protein was modified to necessitate trypsin-dependent maturation of the glycoprotein. HA, NA and M2 were efficiently incorporated in HIV vector particles which could transduce different cell lines in a trypsin-dependent manner. Results also showed that the presence of avian influenza M2 and NA proteins maximized both vector production and transduction and that transduction was highly sensitive to the specific NA inhibitor oseltamivir (Tamiflu). H5N1 HaNaM-pseudotyped HIV vector system was also adapted for cell-based high throughput screening of drug candidates against influenza virus infection, and its high sensitivity to the specific oseltamivir validates its potential utility in the identification of new influenza inhibitors. Overall, the trypsin-dependent H5N1-pseudotyped HIV vector can mimic avian influenza virus infection processes with sufficient precision to allow for the identification of new antivirals and to study avian influenza virus biology in a lower biosafety level laboratory environment.


Subject(s)
Antiviral Agents/pharmacology , Genetic Engineering , Genetic Vectors/genetics , HIV/genetics , Influenza A Virus, H5N1 Subtype/genetics , Trypsin/metabolism , Amino Acid Sequence , Animals , Cell Line , Drug Evaluation, Preclinical , Enzyme Inhibitors/pharmacology , Genetic Vectors/metabolism , Hemagglutinin Glycoproteins, Influenza Virus/chemistry , Hemagglutinin Glycoproteins, Influenza Virus/metabolism , Humans , Influenza A Virus, H5N1 Subtype/chemistry , Influenza A Virus, H5N1 Subtype/metabolism , Neuraminidase/antagonists & inhibitors , Neuraminidase/chemistry , Neuraminidase/metabolism , Oseltamivir/pharmacology , Transduction, Genetic , Viral Proteins/antagonists & inhibitors , Viral Proteins/chemistry , Viral Proteins/metabolism
18.
RSC Adv ; 8(73): 41976-41985, 2018 Dec 12.
Article in English | MEDLINE | ID: mdl-35558777

ABSTRACT

Precise determination of the endogenous catecholamines, dopamine (DA), epinephrine (E) and norepinephrine (NE) faces substantial challenges due to their low physiological concentrations in plasma. We synthesized, for the first time, a magnetic metal-organic framework (MIL-100) composite with boronic acid-functionalized pore-walls (denoted as MG@MIL-100-B composite) using a metal-ligand-fragment coassembly (MLFC) strategy. The composites were then applied as an effective magnetic solid-phase extraction (SPE) sorbent for determination of trace catecholamine concentrations in rat plasma through coupling with HPLC-MS/MS. The obtained nano-composites exhibited high magnetic responsivity, uniform mesopores, large specific surface area, and boronic acid-functionalized inner pore-walls. Catecholamines in rat plasma were extracted through interaction between the cis-diol structures and the boronic acid groups in the MG@MIL-100-B composites. Extraction conditions were optimized by studying SPE parameters including adsorption and desorption time, elution solvent type, pH conditions and adsorbent amount. With our approach, the detection limits (S/N = 3) were as low as 0.005 ng mL-1 for DA and E, and 0.02 ng mL-1 for NE. Intra- and inter-day precision ranged from 2.84-6.63% (n = 6) and 5.70-11.44% (n = 6), respectively. Recoveries from spiking experiments also showed satisfactory results of 94.40-109.51%. Finally, the MG@MIL-100-B composites were applied successfully to determine catecholamine concentrations in rat plasma.

19.
Food Chem ; 239: 612-621, 2018 Jan 15.
Article in English | MEDLINE | ID: mdl-28873612

ABSTRACT

In this study, magnetic graphene/mesoporous silica composites with boronic acid-functionalized pore-walls were synthesized for the first time by a two-step post-graft method. The obtained nano-composites were proven to hold many attractive features such as large specific surface area, uniform mesopores, high magnetic responsibility, and boronic acid-functionalized inner pore-walls. Aminoglycoside residues in milk were extracted using MG@mSiO2-APB composites as restricted access matrix dispersive solid phase extraction adsorbents through the interaction between boronic acid groups and glucoside structures. Extraction conditions were optimized by studying the SPE parameters. Limits of detection of the method were as low as 5ngmL-1 for streptomycin) and 2ngmL-1 for dihydrostreptomycin. Finally, magnetic graphene/mesoporous silica composites with boronic acid-functionalized pore-walls were successfully applied to residue analysis in milk samples. Compared to the traditional extraction methods, using this nano-composites for aminoglycoside residues analysis in milk is more sensitive, effective and convenient.


Subject(s)
Milk , Aminoglycosides , Animals , Boronic Acids , Graphite , Porosity , Silicon Dioxide , Solid Phase Extraction
20.
J Chromatogr A ; 1465: 20-9, 2016 Sep 23.
Article in English | MEDLINE | ID: mdl-27575922

ABSTRACT

In this work, phenyl-functionalized magnetic graphene/mesoporous silica composites (MG-mSiO2-Ph) were prepared and applied as restricted access matrix solid phase extraction (RAM-SPE) adsorbents to determine the parabens in commercially available retail cosmetics. MG-mSiO2-Ph composites were synthesized by a surfactant-mediated co-condensation reaction in which mesoporous silica with phenyl-functionalized pore-walls was coated on a magnetic graphene sheet. The obtained nano-composites were proven to be of sufficient quality for an ideal RAM-SPE adsorbent with a large specific surface area of 369m(2)g(-1), uniform mesopores of 2.8nm, and special phenyl-functionalized pore-walls. Parabens, such as methyl paraben, ethyl paraben and propyl paraben, were extracted from water-based skin toners using one step of the RAM-SPE and were then analysed by a HPLC-DAD system. The SPE conditions were optimized by studying the parameters, such as the adsorbent amount, elution solvent type, adsorption time and desorption time, that influence the extraction efficiency. For each analyte, there were good linearities of approximately 0.10-120µgmL(-1) with determination coefficients (R(2))>0.995. The sensitivity was as low as 0.01-0.025µgmL(-1) for the LOD, and the percent recoveries were 98.37-105.84%. The intra-day and inter-day RSDs were 1.44-6.11% (n=6) and 3.12-11.70% (n=6), respectively. The results indicated that this method with novel RAM-SPE adsorbents is sensitive and convenient. The results also offered an attractive alternative for the extraction and determination of paraben preservatives in a complex matrix, such as cosmetics.


Subject(s)
Chemistry Techniques, Analytical/methods , Cosmetics/chemistry , Graphite/chemistry , Magnetics , Parabens/isolation & purification , Silicon Dioxide/chemistry , Solid Phase Extraction , Adsorption , Chemistry Techniques, Analytical/instrumentation , Chromatography, High Pressure Liquid , Limit of Detection , Parabens/analysis , Solvents/chemistry , Water/chemistry
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