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Cas12a-based on-site, rapid detection of genetically modified crops.
Duan, Zhiqiang; Yang, Xiaoliang; Ji, Xingkun; Chen, Ying; Niu, Xiaomu; Guo, Anping; Zhu, Jian-Kang; Li, Feng; Lang, Zhaobo; Zhao, Hui.
J Integr Plant Biol ; 64(10): 1856-1859, 2022 Oct.
Article in English | MEDLINE | ID: mdl-35962717

ABSTRACT

A CRISPR/LbCas12a-based nucleic acid detection method that uses crude leaf extracts as samples and is rapid (≤40 min for a full run) and highly sensitive (0.01%) can be used to monitor genetically modified organisms in the field.


Subject(s)
CRISPR-Cas Systems , Nucleic Acids , CRISPR-Cas Systems/genetics , Crops, Agricultural/genetics , Plants, Genetically Modified/genetics , Plant Extracts , Nucleic Acid Amplification Techniques/methods
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