ABSTRACT
Possible influence of man-made load on formation and function of microbiocenosis of the nose mucosa in practically healthy people of industrial city has been studied. Microbiota composition, content of secretory immunoglobulin A, functional state and expression of TLR-2 and TLR-4 by mucosa cells were studied in the given human biotope. The residing in conventionally contaminated districts of the industrial city tells on the increase of ecologic role of Candida albicans in the composition of microbiocenosis of the nose mucosa. The authors have shown high sensitivity of the number of TLR-4-positive epithelial cells to the type of the basic taxon of a microorganism in the composition of the nose mucosa microbiota and also sensitivity of the number of TLR-2 and TLR-4-positive epithelial cells and the density of expression by TLR-4 epithelial cells to the man-made pollution, they have also established the expressed unbalance of the system of inherent immunity of the nose mucosa of practically healthy young persons in conditions of man-made pollution.
Subject(s)
Carrier State , Environmental Pollution , Epithelial Cells/immunology , Granulocytes/immunology , Immunity, Innate , Nasal Mucosa/immunology , Adolescent , Candida albicans/growth & development , Carrier State/immunology , Carrier State/microbiology , Epithelial Cells/microbiology , Gene Expression , Granulocytes/microbiology , Host-Pathogen Interactions/immunology , Humans , Immunoglobulin A/metabolism , Male , Microbiota/immunology , Nasal Mucosa/microbiology , Staphylococcus/growth & development , Streptococcus/growth & development , Toll-Like Receptor 2/genetics , Toll-Like Receptor 2/immunology , Toll-Like Receptor 4/genetics , Toll-Like Receptor 4/immunology , Ukraine , Urban Population , Young AdultABSTRACT
We investigated the influence of multiple introductions of NO precursor L-arginine and NOS non-selective blocker N-nitroL-arginine (NNLA) on thymic morpho-functional status in Wistar male rats with experimental diabetes mellitus (EDM). To reveal insulin-expressing, proliferating, Treg-cells, iNOS(+)-cells and Bcl-2(+)-cells, the immunohistochemical methods of direct and indirect immunofluorescence with monoclonal antibodies to insulin, PCNA, CD-25 antigen. Bcl-2 and iNOS of rat were used. It was established that NNLA administration to rats with EDM has more pronounced effect in comparison with L-arginine administration, demonstrating an increase in the number of Treg-cells, insulin-expressing and proliferating thymocytes and a decrease in the density of iNOS(+)- and Bcl-2(+)-cells population.