Dietary selenium affects host selenoproteome expression by influencing the gut microbiota.

Colonization of the gastrointestinal tract and composition of the microbiota may be influenced by components of the diet, including trace elements. To understand how selenium regulates the intestinal microflora, we used high-throughput sequencing to examine the composition of gut microbiota of mice maintained on selenium-deficient, selenium-sufficient, and selenium-enriched diets. The microbiota diversity increased as a result of selenium in the diet. Specific phylotypes showed differential effects of selenium, even within a genus, implying that selenium had unique effects across microbial taxa. Conventionalized germ-free mice subjected to selenium diets gave similar results and showed an increased diversity of the bacterial population in animals fed with higher levels of selenium. Germ-free mice fed selenium diets modified their selenoproteome expression similar to control mice but showed higher levels and activity of glutathione peroxidase 1 and methionine-R-sulfoxide reductase 1 in the liver, suggesting partial sequestration of selenium by the gut microorganisms, limiting its availability for the host. These changes in the selenium status were independent of the levels of other trace elements. The data show that dietary selenium affects both composition of the intestinal microflora and colonization of the gastrointestinal tract, which, in turn, influence the host selenium status and selenoproteome expression.

An effective numerical method for solving viscous-inviscid interaction problems.

This paper presents a new numerical method to solve the equations of the asymptotic theory of separated flows. A number of measures was taken to ensure fast convergence of the iteration procedure, which is employed to treat the nonlinear terms in the governing equations. Firstly, we selected carefully the set of variables for which the nonlinear finite difference equations were formulated. Secondly, a Newton-Raphson strategy was applied to these equations. Thirdly, the calculations were facilitated by utilizing linear approximation of the boundary-layer equations when calculating the corresponding Jacobi matrix. The performance of the method is illustrated, using as an example, the problem of laminar two-dimensional boundary-layer separation in the flow of an incompressible fluid near a corner point of a rigid body contour. The solution of this problem is non-unique in a certain parameter range where two solution branches are possible.