ABSTRACT
Single exposure of white outbred rats to electromagnetic radiation with a frequency 905 MHz (GSM frequency) for 2 h increased anxiety, reduced locomotor, orientation, and exploration activities in females and orientation and exploration activities in males. Glucocorticoid levels and antioxidant system activity increased in both males and females. In addition to acute effects, delayed effects of radiation were observed in both males and females 1 day after the exposure. These results demonstrated significant effect of GSM-range radiation on the behavior and activity of stress-realizing and stress-limiting systems of the body.
Subject(s)
Anxiety/blood , Electromagnetic Radiation , Exploratory Behavior/radiation effects , Freezing Reaction, Cataleptic/radiation effects , Motor Activity/radiation effects , Orientation/radiation effects , Adult , Animals , Animals, Outbred Strains , Antioxidants/metabolism , Anxiety/physiopathology , Cell Phone , Female , Glucocorticoids/blood , Humans , Male , RatsABSTRACT
Adrenocorticotropic hormone (ACTH)-like peptide immunocortin (IMC) VKKPGSSVKV, corresponding to the amino acid sequence 11-20 of the variable part of human immunoglobulin G (IgG) 1 heavy chain, at concentrations of 10(-9)-10(-6) I was found to increase the adenylate cyclase activity in adrenal cortex membranes, while intramuscular injection of immunocortin at doses of 10-100 microg/kg was found to stimulate the secretion of 11-oxycorticosteroids (CS) from the adrenals to the bloodstream. Immunocortin was labeled with tritium to specific activity of 22 Ci/mmol. Receptor binding studies revealed that [(3)H]immunocortin ([(3)H]IMC) bound with high affinity and specificity to ACTH receptors on rat adrenal cortex membranes (K(d)=2.1+/-0.2 nM, B(max)=1.1+/-0.1 pmol/mg protein).
Subject(s)
11-Hydroxycorticosteroids/metabolism , Adrenal Cortex/metabolism , Immunoglobulin G/chemistry , Peptide Fragments/chemistry , Peptides/chemistry , Receptors, Corticotropin/metabolism , Adrenal Cortex/drug effects , Adrenal Glands/metabolism , Amino Acid Sequence , Animals , Binding, Competitive , Cell Membrane/metabolism , Cyclic AMP/metabolism , Dose-Response Relationship, Drug , Immunoglobulin G/metabolism , Immunoglobulin Variable Region/chemistry , Kinetics , Molecular Sequence Data , Protein Binding , Rats , Sensitivity and Specificity , Time Factors , Tritium/chemistryABSTRACT
We studied the effect of acute antenatal hypoxia during the stages of progestation and early organogenesis on some ECG parameters and level of biogenic amines in brain structures in rats. The effect of acute hypoxic exposure during the organogenesis period on the studied parameters was more pronounced than the effect of acute hypoxic exposure during the progestation period. The shift of the autonomic balance towards the sympathetic regulation of cardiac activity is linked with increased content of biogenic amines in the brain stem and cortical structures.
Subject(s)
Biogenic Amines/metabolism , Central Nervous System/metabolism , Fetal Hypoxia/physiopathology , Heart/physiopathology , Age Factors , Animals , Autonomic Nervous System/physiopathology , Brain Stem/metabolism , Cerebral Cortex/metabolism , Dopamine/metabolism , Electrocardiography , Female , Fetal Hypoxia/metabolism , Male , Norepinephrine/metabolism , Pregnancy , Prenatal Exposure Delayed Effects/metabolism , Prenatal Exposure Delayed Effects/physiopathology , Rats , Serotonin/metabolismABSTRACT
Cardiac activity in rats during the postnatal period was studied in vitro and in vivo after exposure of rat pups to antenatal acute hypobaric hypoxia at the stage of organogenesis (day 9-10 of gestation). Cultured cardiomyocytes from rat pups exposed to antenatal hypoxia were characterized by increased rate of contractions and decreased reactivity to norepinephrine. Heart rate elevation, predominance of sympathetic influences on cardiac activity, and significant increase in norepinephrine concentration in the cerebral cortex were found in freely moving animals exposed to antenatal hypoxia. Our results indicate that hypoxia at the stage of organogenesis modulated cardiac activity during the postnatal period, which manifested at the level of effector structures in the heart and activity of regulatory systems.
Subject(s)
Hypoxia/physiopathology , Myocytes, Cardiac/physiology , Norepinephrine/physiology , Prenatal Exposure Delayed Effects , Animals , Animals, Newborn , Cells, Cultured , Cerebral Cortex/metabolism , Female , Fetal Hypoxia/physiopathology , Heart Rate/drug effects , Myocardial Contraction/drug effects , Myocytes, Cardiac/drug effects , Pregnancy , Pregnancy Complications , RatsABSTRACT
We studied the effect of exposure to acute hypobaric hypoxia in the progestational period on the content of biogenic amines in the brainstem and cerebral cortex in rat pups of different age. The possibility of correcting hypoxia-induced changes with regulatory peptides was evaluated. We found that early antenatal hypoxia disturbs maturation of catecholaminergic systems in the brain. It should be emphasized that the differences from the control varied depending on the age of rat pups. Single intranasal administration of Semax heptapeptides and beta-casomorphine-7 to pregnant females prevented changes in the content of biogenic amines in CNS of the offspring during postnatal ontogeny.
Subject(s)
Biogenic Amines/metabolism , Brain Stem/metabolism , Cerebral Cortex/metabolism , Hypoxia/metabolism , Peptides/metabolism , Aging/physiology , Animals , Animals, Newborn , Atmospheric Pressure , Biogenic Amines/chemistry , Brain Chemistry , Female , Peptides/administration & dosage , Peptides/chemistry , Pregnancy , RatsABSTRACT
beta-Endorphin-like decapeptide immunorphin (SLTCLVKGFY), a selective agonist of non-opioid beta-endorphin receptor, was labeled with tritium to specific activity of 24 Ci/mmol. It was used for the detection and characterization of non-opioid beta-endorphin receptors on rat adrenal cortex membranes (Kd1 = 39.6 +/- 2.0 nM, Bmax1 = 40.7 +/- 2.3 pmol/mg protein; Kd2 = 0.25 +/- 0.01 micro M, Bmax2 = 187.8 +/- 9.4 pmol/mg protein). beta-Endorphin was found to inhibit the [3H]immunorphin specific binding to membranes (Ki = 70.0 +/- 9.2 nM); naloxone, [Met5]enkephalin, and alpha- and gamma-endorphins tested in parallel were inactive. Immunorphin at concentrations of 10(-9)-10(-6) M was found to inhibit the adenylate cyclase activity in adrenocortical membranes, while intramuscular injection of immunorphin at doses of 10-100 micro g/kg was found to reduce the secretion of 11-oxycorticosteroids from the adrenals to the bloodstream.