ABSTRACT
â¢Four newly recorded species of Podostemaceae from southern China were identified by molecular and morphological evidence.â¢17 plastomes of Podostemaceae were newly sequenced and two novel polymorphic barcodes (ccsA and ndhA) detected.â¢Our findings reveal greater species richness (15 species from five genera) of Podostemaceae in China and supply molecular resources for research on taxonomy and phylogenomics of this enigmatic aquatic family.
ABSTRACT
In the present study, we announce the first complete chloroplast genome sequence of Artocarpus tonkinensis, a tree native to China with diverse beneficial uses. This complete chloroplast genome is 160,987 bp in length. In total, 130 genes were identified, including 85 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. The findings of phylogenetic analysis supported that Artocarpus belongs to the Moraceae family and proposed a sister relationship between Artocarpus and Morus.
ABSTRACT
Diet and exercise are conventional methods for controlling body weight and are linked to alterations in gut microbiota. However, the associations of diet, exercise, and gut microbiota in the control of obesity remain largely unknown. In the present study, using 16S rRNA amplicon sequencing and fecal microbiota transplantation (FMT), normal fat diet (NFD), exercise and their combination resulted in improved metabolic profiles in comparison to sedentary lifestyle with high fat diet (HFD). Moreover, diet exerted more influence than exercise in shaping the gut microbiota. HFD-fed mice receiving FMT from NFD-exercised donors not only showed remarkably reduced food efficacy, but also mitigated metabolic profiles (p < 0.05). The transmissible beneficial effects of FMT were associated with bacterial genera Helicobacter, Odoribacter and AF12 and overrepresentation of oxidative phosphorylation and glycolysis genes. Our findings demonstrate that the beneficial effects of diet and exercise are transmissible via FMT, suggesting a potential therapeutic treatment for obesity.
Subject(s)
Diet, High-Fat , Fecal Microbiota Transplantation , Physical Conditioning, Animal , Animals , Feeding Behavior , Gastrointestinal Microbiome , Gene Expression Regulation , Inflammation/genetics , Male , Mice, Inbred C57BL , Mice, Obese , Principal Component AnalysisABSTRACT
The first microRNA, let-7, and its family were discovered in Caenorhabditis elegans and are functionally conserved from worms to humans in the regulation of embryonic development and stemness. The let-7 family has been shown to have an essential role in stem cell differentiation and tumor-suppressive activity. Deregulating expression of let-7 is commonly reported in many human cancers. Emerging evidence has accumulated and suggests that reestablishment of let-7 in tumor cells is a valuable therapeutic strategy. However, findings reach beyond tumor therapeutics and may impinge on stemness and differentiation of stem cells. In this review, we discuss the role of let-7 in development and differentiation of normal adult stem/progenitor cells and offer a viewpoint of the association between deregulated let-7 expression and tumorigenesis. The regulation of let-7 expression, cancer-relevant let-7 targets, and the application of let-7 are highlighted.