Clathrin light chains regulate hypocotyl elongation by affecting the polarization of the auxin transporter PIN3 in Arabidopsis.

PIN-FORMED (PIN)-dependent directional auxin transport is crucial for plant development. Although the redistribution of auxin mediated by the polarization of PIN3 plays key roles in modulating hypocotyl cell expansion, how PIN3 becomes repolarized to the proper sites within hypocotyl cells is poorly understood. We previously generated the clathrin light chain clc2-1 clc3-1 double mutant in Arabidopsis thaliana and found that it has an elongated hypocotyl phenotype compared to the wild type. Here, we performed genetic, cell biology, and pharmacological analyses combined with live-cell imaging to elucidate the molecular mechanism underlying the role of clathrin light chains in hypocotyl elongation. Our analyses indicated that the defects of the double mutant enhanced auxin maxima in epidermal cells, thus, promoting hypocotyl elongation. PIN3 relocated to the lateral sides of hypocotyl endodermal cells in clc2-1 clc3-1 mutants to redirect auxin toward the epidermal cell layers. Moreover, the loss of function of PIN3 largely suppressed the long hypocotyl phenotype of the clc2-1 clc3-1 double mutant, as did treatment with auxin transport inhibitors. Based on these data, we propose that clathrin modulates PIN3 abundance and polarity to direct auxin flux and inhibit cell elongation in the hypocotyl, providing novel insights into the regulation of hypocotyl elongation.

Is there gender inequality in the impacts of energy poverty on health?

This paper aims to study the gender inequality in the health impacts of energy poverty. Using the Chinese Family Panel Studies (CFPS) 2018 data, we examine the gender heterogeneous effects of energy poverty on residents' health. The empirical results indicate that energy poverty would increase the ratio of respiratory disease, hospitalization as well as the healthcare expenditure. The effects are moderated by housework time. Moreover, we explore the gender inequality of the health impacts and find that women's health is more severely impaired and the effect of gender inequality is heterogeneous between urban and rural areas. We further investigate the historical origins of intra-household division of labor and reveal that the root of gender inequality in the health effects of energy poverty is status inequality. The government should give the first priority to energy poverty alleviation through modern energy network construction. Providing the energy-deprived families with ventilation equipment and medical insurance should be considered as the next step. Finally, it is imperative to eradicate urban-rural dual structure and legislate to ensure gender equality in the whole society.