1.
Angew Chem Int Ed Engl
; 56(10): 2776-2779, 2017 03 01.
Article
in English
| MEDLINE
| ID: mdl-28146322
ABSTRACT
The mechanisms of two diterpene cyclases from streptomycetes-one with an unknown product that was identified as the spirocyclic hydrocarbon spiroviolene and one with the known product tsukubadiene-were investigated in detail by isotope labeling experiments. Although the structures of the products were very different, the cyclization mechanisms of both enzymes proceed through the same initial cyclization reactions, before they diverge towards the individual products, which is reflected in the close phylogenetic relationship of the enzymes.