Involvement of the prostate and testis expression (PATE)-like proteins in sperm-oocyte interaction.

BACKGROUND: The prostate and testis expression (PATE)-like family of proteins are expressed mainly in the male genital tract. They are localized in the sperm head and are homologous to SP-10, the acrosomal vesicle protein also named ACRV1. Our aim was to characterize the expression and functional role of three PATE-like proteins in the testis and ejaculated sperm. METHODS: The expression and localization of PATE-like proteins in human testis biopsies (n= 95) and sperm cells were assessed by RT-PCR, immunohistochemistry and immunofluorescence staining (at least 600 sperm cells per specimen). The function of the PATE protein was tested by the hemizona assay and hamster egg penetration test (HEPT). RESULTS: PATE and PATE-M genes and proteins were present almost exclusively in germ cells in the testis: immunoflourescence showed that the percentage of germ cells positive for PATE, PATE-M and PATE-B was 85, 50 and 2%, respectively. PATE and PATE-M proteins were localized in the equatorial segment of the sperm head, while PATE-B protein was localized in the post-acrosomal region. A polyclonal antibody (Ab, at 1:50 and 1:200 dilutions) against the PATE protein did not inhibit sperm-zona binding in the hemizona assay (hemizona index of 89.6 ± 10 and 87 ± 36%, respectively). However, there was inhibition of sperm-oolemma fusion and penetration in the HEPT (penetration index: without Ab 7 ± 3.9; Ab dilution of 1:100, 4 ± 3.5; Ab dilution of 1:20, 0.6 ± 1.2, P < 0.001). CONCLUSIONS: Our data suggest that PATE protein is involved in sperm-oolemma fusion and penetration but not sperm-zona binding.

Adoptive transfer of small numbers of DX5+ cells alleviates graft-versus-host disease in a murine model of semiallogeneic bone marrow transplantation: a potential role for NKT lymphocytes.

Natural killer T (NKT) lymphocyte cells are a subset of regulatory lymphocytes with important immunemodulatory effects. Our aim was to evaluate the effect of transplantation of NKT lymphocytes on graft versus host disease (GVHD) in a murine model of semiallogeneic BMT. GVHD was generated by infusion of 2 x 107 splenocytes from C57BL/6 donor mice into irradiated (C57BL/6 x Balb/c)F1 recipient mice. Adoptive transfer of increasing numbers of DX5+ cells was performed. Recipient mice were followed for histological parameters of GVHD-associated liver, bowel, and cutaneous injury. Intrahepatic and intrasplenic lymphocytes were isolated and analyzed by FACS for CD4+ and CD8+ subpopulations. It was seen that adoptive transfer of 4.5 x 106 DX5+ cells significantly alleviated GVHD-related hepatic, bowel, and cutaneous injury, and improved survival (85% survival on day 28). In contrast, depletion of DX5+ cells led to severe GVHD-associated multiorgan injury and 100% mortality. A direct correlation with the number of transplanted DX5+ cells was noted (maximal effect with transplantation of 4.5 x 106 DX5+ cells). Tolerance induction was associated with an increased peripheral CD4/CD8 ratio, intrahepatic trapping of CD8 lymphocytes and a shift towards a Th2-type cytokine profile, manifested by decreased IL-12/IL10, IL-12/IL-4, IFNgamma/IL-10, and IFNgamma/IL-4 ratios. Transplantation of DX5+ cells holds promise as a novel therapeutic measure for GVHD.

Cross phase modulation squeezing in optical fibers.

A novel method of producing squeezed vacuum uses cross phase modulation between a linearly polarized pump signal and the orthogonal polarized vacuum. Here we report on such cross phase modulation using 1-nJ 150-fs pulses from a low noise stretched pulse laser. The nonlinear medium was a single mode fiber and the noise reduction was 3 dB.

Mothers' perceptions of anxiety of their diabetic children.

Within the theoretical framework of family dynamics, the anxiety levels expressed by 20 diabetic children, the level of anxiety ascribed to them by their mothers, and the anxiety levels of 20 healthy children were studied. The diabetic children did not form an emotionally deviant group in terms of their expressed anxiety, although their mothers viewed them as significantly more anxious than they judged themselves. The mothers' own anxiety was the best predictor of their perceptions of their children's anxiety. The results are discussed in terms of their effects on communication and coping in the families.

Classification and validation of family climate subtypes in kibbutz fathers of disabled and nondisabled children.

The aim of the study was to investigate the impact of disabled children on their fathers. Perceptions of family climate, sense of coherence, and satisfaction from family life were measured among 66 fathers of disabled children and among 74 fathers with nondisabled children in an Israeli kibbutz. The fathers of disabled children showed lower sense of coherence, derived less satisfaction from family life, and viewed their families as less encouraging of personal growth among family members. Four subtypes of family climate patterns, perceived by fathers of disabled children, were identified by cluster analysis and validated by the sense of coherence and global family satisfaction measures. Cluster analysis for the fathers of nondisabled children replicated the subtypes identified. The four subtypes were conceptualized as families with a (a) personal growth orientation, (b) conflict orientation, (c) conflict avoidance orientation, and (d) recreation avoidance orientation. Significant differences were found between the subtypes with regard to the family climate variables and satisfaction and coherence measures. Results demonstrated that different patterns of family climate characterized the two groups of fathers: In almost every cluster the measures of personal growth were lower for the fathers of disabled children.

Social skills and classroom behavior among adolescents with mild mental retardation.

Relations among self-reported social skills, teacher ratings of classroom behavior, and direct observations of social interactions among 87 adolescents with mild mental retardation were investigated and variables predicting the students' task orientation identified. Three information sources were employed. Results demonstrated that the four domains of social skills were related to the students' task orientation. The regression analysis revealed that students who were characterized by better social skills and fewer occurrences of hyperactivity and behavioral difficulties were viewed by teachers as evidencing more compliant and task-oriented behavior, implying their better ability to benefit from the special curriculum. The three sources of information validated one another and added information to the conceptualization and appraisal of social and academic competence among students with mental retardation.

Loneliness and coherence among preschool children with learning disabilities.

This study investigated loneliness and coherence among Israeli preschool children with learning disabilities, in an attempt to identify the sources of social deficits before academic failure was established. The sample consisted of 187 preschool children divided into three groups: (a) 60 children at high risk for developing learning disabilities (LD) in six mainstreamed preschool settings (47 boys and 13 girls), (b) 76 nonhandicapped peers from the same preschools (56 boys and 20 girls), and (c) 51 children (38 boys and 13 girls) at high risk for developing LD who were regular students at 17 preschools and received special help in the afternoons outside their educational settings, at a regional learning center for students with LD. The research instruments consisted of the Children's Sense of Coherence Scale, the Loneliness Scale, a peer nomination procedure, and teachers' ratings. Two-way MANOVAs demonstrated that the two groups of children (Groups [A] and [C]) with LD and with a high risk for developing learning disabilities experienced higher levels of loneliness and lower levels of coherence. A subgroup examination revealed that they were less accepted by nondisabled peers and had less reciprocal nominations. Furthermore, their teachers viewed them as showing more maladjustment.

Academic competence and social adjustment of boys with learning disabilities and boys with behavior disorders.

The aim of the present study was to compare academic competence and social adjustment of students with learning disabilities and two groups of students with behavior disorders. The sample consisted of 31 boys with learning disabilities and 52 boys with behavior disorders divided into two subgroups: (a) boys displaying hyperactive behavior (BDH) and (b) boys not demonstrating such behavior (BDNH). Significant differences were found among the three groups using the Classroom Behavior Inventory, and three measures contributed to the significant difference: Hostility versus Consideration, Extroversion versus Introversion, and Independence versus Dependence. The results highlighted teachers' needs for differential approach in their interventional planning.

Classroom behavior and family climate in students with learning disabilities and hyperactive behavior.

The present study aimed to identify subtypes of the learning disabilities (LD) syndrome by examining classroom behavior and family climate among four groups of Israeli students ranging in age from 7 to 10 years: 22 students with LD and hyperactive behavior (HB), 22 nonhyperactive students with LD, 20 nondisabled students with HB, and 20 nondisabled nonhyperactive students. Schaefer's Classroom Behavior Inventory and Moos's Family Environmental Scale were administered to teachers and mothers, respectively. The results revealed that higher distractibility and hostility among both groups with HB differentiated between the two groups with LD. Families of children with HB were reported as less supportive and as emphasizing control less. The academic competence and temperament of the nondisabled students with HB were rated as similar to those of the two groups of students with LD. Both groups with LD were characterized by dependent interpersonal relations and by more conflictual families who fostered more achievement but less personal growth.

Behavior disorders and mental retardation: the family system perspective.

The aim of this study was to investigate feelings of stress among parents of mentally retarded children who demonstrate behavior disorders. The interrelations of the children's pathology and family climate variables with the levels of parental stress were studied. The sample consisted of 39 families with moderately mentally retarded children divided into two groups: Children demonstrating disruptive behavior (n = 17), and children who did not demonstrate behavior disorders (n = 22). The instruments included the Child Behavior Checklist, the Classroom Behavior Inventory, the Family Environment Scale, and the Questionnaire on Resources and Stress. Significant differences between the children's levels of pathology validated the two groups' division. The children with disruptive behavior were described by their parents as more hyperactive and aggressive, and were described by their teachers as more distractable and dependent on adults. No significant differences were found between the profiles of family climate among the two groups of parents. However, levels of children's pathology and aspects of family climate predicted the parental stress, pinpointing different patterns of variables for fathers and mothers. Further studies should be addressed to the interrelations between children's pathology, family climate, and parental feelings of stress.

Ethnic differences in expressions of shame feeling by mothers of severely handicapped children.

Shame on the part of parents of mentally handicapped children has pronounced effects on child-rearing practices. The aim of this study was to compare expressions of shame of different ethnic groups in Israel. The attitudes of 23 Western mothers and 26 Eastern mothers towards their moderately and severely retarded children were studied. Significant differences (p less than 0.05-p less than 0.01) were found, suggesting that the Eastern mothers strongly expressed their shame, whereas the Western mothers 'felt ashamed' to express it at all. The Western mothers felt that the social norms that reject feelings of shame and their own personal feelings of embarrassment were in conflict.

Multivariate concept of psychosomatic illness the self-concept of asthmatic children.

The self-concept of asthmatic children was studied in order to demonstrate the complicated nature of the psychosomatic syndrome; that of 18 asthmatic children was compared to those of three other groups': 20 healthy children, 20 children with emotional difficulties and 11 children with cystic fibrosis. Measures reflecting the psychological self-acceptance differentiated the healthy children significantly from the two groups with emotional difficulties (asthmatics, and children with adjustment difficulties). Measures reflecting physical self acceptance differentiated the healthy group significantly from the two groups with somatic complaints (asthmatics and cystic fibrosis children).

Anxiety and self-dissatisfaction in epileptic children.

Twenty epileptic children were compared with regard to their anxiety levels and self-concept measures, to two control groups: (a) non-handicapped children, (b) learning-disabled children. The epileptic children demonstrated higher levels of trait anxiety and dissatisfaction with themselves, as compared to each and both of the control groups. The results stressed the critical value of psychological consultation for the epileptic children and their parents.

[Cholangiocarcinoma in primary sclerosing cholangitis: "chronicle of a death foretold"?].

Primary sclerosing cholangitis (PSC), a condition usually associated with inflammatory bowel disease, is a chronic inflammatory state of the biliary tract characterized by fibrotic strictures of intrahepatic and extrahepatic bile ducts. Between 5-15% of patients with PSC will develop cholangiocarcinoma. The diagnosis of cholangiocarcinoma in patients with PSC is difficult, as it is often impossible to distinguish benign PSC-related strictures from lesions associated with cholangiocarcinoma. Consequently, most patients are diagnosed with advanced disease and have a dismal prognosis at the time of diagnosis. The importance of characterizing patients with PSC who have an increased risk of developing cholangiocarcinoma and of developing means for early detection of this disease cannot be over-emphasized. Detection of cholangiocarcinoma in asymptomatic patients is additionally important, as this condition is considered a contraindication for liver transplantation. We describe a patient with longstanding Crohn's disease and PSC who developed cholangiocarcinoma, and review the literature regarding risk factors for cholangiocarcinoma, early detection of cholangiocarcinoma and the significance of existing cholangiocarcinoma in the context of liver transplantation.