Deep-Seated Fluids in Thermal Waters Before and After the 2016 Kumamoto Earthquakes.

Noble gases, oxygen-hydrogen isotope ratios, and ion compositions were measured at three sampling points (KUM, OTN, and ASO) from December 2013 to July 2021. The 3 He/4 He values at the three sampling points remained stable in the range of 3-4 Ra throughout the observation period, suggesting that the supply of deep-seated gases to the aquifer was stable. The 4 He/20 Ne values of KUM and OTN indicate that the supply of surface-source fluids to the aquifer decreased relative to that of deep-seated fluids at KUM and OTN. In contrast, in the ASO site, both the surface- and deep-seated fluids supplied to the aquifer were stable. The δD-δ18 O relationship indicated the supply of deep-seated water to the KUM and OTN aquifers but not to the ASO aquifer. Nevertheless, the δD-δ18 O relationship remained stable throughout the observation period, suggesting that the supply of deep-seated water to the three stations was stable. The Li/Cl and 1/Cl relationships for the three sampling points were plotted within a narrow range throughout the observation period, suggesting that the groundwater recharge was stable. Neither spikes nor step changes owing to the 2016 Kumamoto earthquake were observed in any of the data. These results indicate that the KUM and OTN aquifers are constantly supplied with deep fluids from the fluid-rich zone beneath the Kumamoto region, and that only deep-seated gas was supplied to the ASO aquifer. We also confirmed that these supply conditions were unaffected by the 2016 Kumamoto earthquake or the subsequent aftershock activity.

Biogeochemical signals from deep microbial life in terrestrial crust.

In contrast to the deep subseafloor biosphere, a volumetrically vast and stable habitat for microbial life in the terrestrial crust remains poorly explored. For the long-term sustainability of a crustal biome, high-energy fluxes derived from hydrothermal circulation and water radiolysis in uranium-enriched rocks are seemingly essential. However, the crustal habitability depending on a low supply of energy is unknown. We present multi-isotopic evidence of microbially mediated sulfate reduction in a granitic aquifer, a representative of the terrestrial crust habitat. Deep meteoric groundwater was collected from underground boreholes drilled into Cretaceous Toki granite (central Japan). A large sulfur isotopic fractionation of 20-60‰ diagnostic to microbial sulfate reduction is associated with the investigated groundwater containing sulfate below 0.2 mM. In contrast, a small carbon isotopic fractionation (<30‰) is not indicative of methanogenesis. Except for 2011, the concentrations of H2 ranged mostly from 1 to 5 nM, which is also consistent with an aquifer where a terminal electron accepting process is dominantly controlled by ongoing sulfate reduction. High isotopic ratios of mantle-derived 3He relative to radiogenic 4He in groundwater and the flux of H2 along adjacent faults suggest that, in addition to low concentrations of organic matter (<70 µM), H2 from deeper sources might partly fuel metabolic activities. Our results demonstrate that the deep biosphere in the terrestrial crust is metabolically active and playing a crucial role in the formation of reducing groundwater even under low-energy fluxes.