ABSTRACT
Cytostatic activity of baicalin, baicalein, and neogalenical drug Chlorophyllipt was studied in vitro on HeLa-v cells. Standard samples of Eucalimin, baicalin, and baicalein, as well as Chlorophyllipt and paclitaxel (reference drug Taxacad) were used. The cell deaths were determined by MTT assay in a Multiskan FC microplate reader with incubator. The effective inhibition concentration (IC50) of the tested substances were: paclitaxel (4.0±0.4 µM)-baicalein (10.5±1.1 µM)-baicalin (16.5±1.7 µM)-sum of euglobals in Chlorophyllipt (24.1±2.5 µM). Chlorophyllipt was found to exhibit cytostatic activity. Cytostatic activity of baicalein, baicalin, and Chlorophyllipt was lower than cytostatic activity of the reference drug by 2.6, 4.1, and 6 times, respectively. The prospects of further evaluation of the synergetic effect of baicalin, baicalein, and chlorophyllipt used in combinations with different cytostatic agents for finding the most effective combination have been shown.
Subject(s)
Cytostatic Agents , Flavanones , Humans , Cytostatic Agents/pharmacology , Flavonoids/pharmacology , Flavanones/pharmacology , Flavanones/metabolism , HeLa Cells , PaclitaxelABSTRACT
We studied the expression of transcription factors RUNX2 and Osterix after addition of a concentrate of osteogenic-conditioned medium to the culture medium for osteogenic differentiation of mesenchymal stem cells (MSC). The obtained concentrate of osteogenic-conditioned medium containing a complex of bioactive substances with a molecular weight >10 kDa provided MSC differentiation into osteoblasts, which was confirmed by high level of expression of transcription factors RUNX2 and Osterix in comparison with the negative control. The highest expression of transcription factor Osterix was revealed on day 14 of MSC culturing in the presence of osteogenic supplement StemPro (positive control) and the studied concentrate of osteogenic-conditioned medium.
Subject(s)
Core Binding Factor Alpha 1 Subunit/genetics , Culture Media, Conditioned/pharmacology , Mesenchymal Stem Cells/drug effects , Osteoblasts/drug effects , Osteogenesis/drug effects , Transcription Factors/genetics , Animals , Ascorbic Acid/pharmacology , Biomarkers/metabolism , Bone Marrow Cells/cytology , Bone Marrow Cells/drug effects , Bone Marrow Cells/metabolism , Cell Differentiation/drug effects , Core Binding Factor Alpha 1 Subunit/metabolism , Culture Media, Conditioned/chemistry , Dexamethasone/pharmacology , Gene Expression , Glycerophosphates/pharmacology , Mesenchymal Stem Cells/cytology , Mesenchymal Stem Cells/metabolism , Osteoblasts/cytology , Osteoblasts/metabolism , Osteogenesis/genetics , Primary Cell Culture , Rats , Rats, Wistar , Transcription Factors/metabolismABSTRACT
We studied the influence of the characteristics of oxide porous coatings on osteogenesis and integration of new bone tissue and titanium implant surface in the ectopic osteogenesis test. Implants with coating with pore size 2-20 µ exhibit better osteogenic and osteoconductive characteristics than implants with homogenous surface and smooth relief (pore size 0.5-5.0 µ). Physical characteristics of the surface of the material along with chemical composition of the coating are essential for the formation of bone tissue and its integration with the implant.
Subject(s)
Bone Marrow Cells/drug effects , Bone-Implant Interface/anatomy & histology , Calcium Compounds/pharmacology , Coated Materials, Biocompatible/pharmacology , Osteoblasts/drug effects , Oxides/pharmacology , Titanium/pharmacology , Animals , Bone Marrow Cells/cytology , Bone Marrow Cells/physiology , Bone-Implant Interface/physiology , Cell Differentiation , Choristoma/diagnostic imaging , Dental Implants , Male , Osteoblasts/cytology , Osteoblasts/physiology , Osteogenesis/drug effects , Porosity , Primary Cell Culture , Rats , Rats, Wistar , Surface Properties , Titanium/chemistryABSTRACT
The osteoinductive characteristics of hydroxylapatite-based coating with different physicochemical characteristics were studied. The formation of the bone tissue on the studied types of the implant coating differed by the pattern and rate of formation of structural elements of the bone. Clear-cut phase-wise osteogenesis was seen on combined calcium phosphate coating: intense formation of the fibrous interstitial substance (due to cellular structure of hydroxylapatite) and slow maturing of cell elements. Bilayer composite coating provided (during the same period) the formation of more mature connective tissue. This phenomenon can be explained by better bioavailability of the coating material. The properties of combined calcium phosphate coating can be evaluated as mainly osteoconductive, while those of bilayer composite coating as osteoinductive.
Subject(s)
Coated Materials, Biocompatible , Durapatite , Osteogenesis/physiology , Animals , Bone and Bones/cytology , Bone and Bones/pathology , Bone and Bones/physiology , Coated Materials, Biocompatible/chemistry , Coated Materials, Biocompatible/metabolism , Durapatite/chemistry , Durapatite/metabolism , Implants, Experimental , Materials Testing , Random Allocation , Rats , Surface Properties , Titanium/chemistryABSTRACT
The possibility of using the atomic-force microscopy for the evaluation of the geometrical characteristics of blood cells has been shown. It has been found by comparing the hemocyte morphometric indices obtained at different modes of scanning that noncontact and semicontact methods are adequate for the estimation of the size and geometry of biological objects. The scanning of cells in the semicontact mode leads to the irreversible deformation of samples.
Subject(s)
Erythrocytes/cytology , Lymphocytes/cytology , Animals , Cell Size , Chickens , Microscopy, Atomic Force , RatsABSTRACT
Iron-deficiency anemia in pregnant females negatively affects pregnancy, delivery, neonatal infants. Anemia-caused placental changes found during pregnancy are characterized by imbalance between the processes of adaptation and dysadaptation in response to the developed hypoxia in the pregnant-placenta-fetus system and placental tissue ischemia. When iron-deficiency anemia had developed before pregnancy, placental morphological changes show the signs of chronic placental insufficiency. The developed therapy for anaemia with Ferro-Folgama corrects all the elements of the pregnant-placenta-fetus system.
Subject(s)
Anemia, Iron-Deficiency/pathology , Placenta/pathology , Pregnancy Complications, Hematologic/pathology , Anemia, Iron-Deficiency/blood , Anemia, Iron-Deficiency/complications , Anemia, Iron-Deficiency/drug therapy , Apgar Score , Birth Weight , Female , Humans , Infant, Newborn , Placenta/ultrastructure , Pregnancy , Pregnancy Complications, Hematologic/blood , Pregnancy Complications, Hematologic/drug therapy , Pregnancy Outcome , Prospective StudiesABSTRACT
Based on the method of hypoosmotic loads, the value of membrane reserve and its use by blood cells of reptiles and mammals have been studied. It has been shown that lymphocytes of the both animal species have the highest reserve of plasmalemma, while frog heterophils - the lowest one. A significantly lower part of the membrane reserve participates in formation of phagosomes by mammalian neutrophils as compared with amphibian erythrocytes.