Prostaglandin synthesis enzymes' gene transcription in bitches with endometritis.

Endometritis is a major cause of infertility in many domestic species. However, until now the pathogenesis of the endometritis in the bitch is unclear. The aim of this study was to evaluate the gene transcription pattern of prostaglandin (PG) synthesis enzymes (cyclooxygenase [COX2], PTGES-1 and PGFS) in the endometrium of bitches with or without endometritis. Thirty mixed breed bitches in dioestrus, aged between 1 and 5 years, and weighing between 10 and 30 kg were used. After ovariohysterectomy (OVX), uterine biopsy samples were collected from the middle part of both horns. Then, endometrial epithelium was collected using the cytobrush method and mRNA analysis was performed by real-time RT-PCR. Data were analysed with Kruskal-Wallis anova using the sas® software. Uterine condition was identified by endometrial biopsies (normal endometria [n = 11; NE], acute endometritis [n = 10; AE] and chronic endometritis [n = 9; CE]). The COX2, PTGES-1 and PGFS/AKR1C3 mRNA expression in bitches with and without endometritis was similar. Except for PGFS/AKR1C3, gene transcription of COX2 and PTGES-1 was significantly increased in AE compared with CE. In addition, COX2 gene transcription was significantly increased in AE compared with NE. In contrast, no differences were found for COX2, PTGES-1 and PGFS/AKR1C3 mRNA expression in the samples of NE compared with CE.

Prolactin, Androstenedione and IGF1 Serum Concentrations During Induced Follicular Growth by eCG Administration in the Bitch.

The oestrus cycle in the domestic bitch, a monoestrous species, differs considerably from that of other veterinary domestic animals species. In the bitch the combined use of eCG and hCG is effective to induce oestrus predictably and safely (Stornelli et al., Theriogenology, 78, 2012 and 1056). Although several studies were done to describe the hormonal changes during the canine oestrus cycle, to our knowledge none was done to describe the hormonal changes during induced follicular growth after the administration of eCG. The aim of this work was to study prolactin (PRL), insulin-like growth factor (IGF1) and androstenedione (ANDR) serum concentrations during follicular growth induced by a single dose of eCG administered to late anoestrous bitches. PRL and ANDR concentrations were lower before than after eCG TRT (before eCG vs pro-oestrus, oestrus and dioestrus; 4.3 ± 1.8 ng/ml vs 6.5 ± 1.6 ng/ml, p < 0.05; 0.08 ± 0.2 ng/ml vs 0.42 ± 0.16 ng/ml, p < 0.05). Conversely, IGF1 concentrations were similar before and after eCG TRT (286.0 ng/ml ±32.2, p > 0.53). Additionally, PRL concentrations were similar before oestrus compared to during oestrus and dioestrus (6.9 ± 1.7 ng/ml, p > 0.19). Furthermore, IGF1 concentrations were higher before and during oestrus compared to first day of dioestrus (286.1 ± 29.8vs 200.4 ± 29.2 ng/ml, p < 0.01). On the contrary, ANDR concentrations were lower before and during oestrus compared to first day of diestrum (0.35 ± 0.17 ng/ml and 0.38 ± 0.15 vs 0.68 ± 0.17 ng/ml, p < 0.05). These results show that treatment with a single injection of 50 IU/kg of eCG in late anoestrous bitches successfully induced changes in follicular growth which were paralleled with changes in PRL, IGF1 and ANDR serum concentration similar to those occurring during a normally occurring oestrous cycle. In addition, our results suggest that IGF1 in the bitch could play an important role in ovarian folliculogenesis.

Efficacy of cloprostenol or aglepristone at 21-22 and 35-38 days of gestation for pregnancy termination in queens.

The objective of this study was to evaluate the efficacy of cloprostenol (CLO) or aglepristone (ALI) for pregnancy termination in queens at 21-22 and 35-38 days of gestation. Two experiments (EXP) were carried out to accomplish this aim. Thirty-seven 12- to 14-month-old mixed breed queens were used in a randomized design. At oestrus, queens were housed for mating with a tom, and pregnancy was confirmed by transabdominal ultrasonographic examination (US). On days 21-22 of pregnancy (EXP1) or 35-38 of pregnancy (EXP2), queens were divided into three groups (G). Queens in G1 received ALI (10 mg/kg, sc; EXP1, n = 6; EXP2, n = 6) on two consecutive days. Queens in G2 received CLO (5 µg/kg, sc; EXP1, n = 6; EXP2 = 7) on three consecutive days. Queens in G3 received 1 ml of saline solution (PLA, sc; EXP1, n = 6; EXP2 = 6). After treatment, females were monitored daily by US during for 10 days and weekly until the end of gestation. In EXP1, pregnancy was terminated in (6/6, 100%), (0/6, 0%) and (0/6, 0%), for the ALI, CLO and PLA groups, respectively (p < 0.001). In EXP2, pregnancy was terminated in (6/6, 100%), (1/7, 14%) and (0/6, 0%) for the ALI, CLO and PLA groups, respectively (p < 0.001). In both EXP, after CLO administration, animals vomited and were depressed for 30 min; but no side effects were observed in the animals in the ALI group. In conclusion, the results from this study indicate that three injections of CLO are not effective, but two injections of ALI are effective to induce abortion in queens at 21-22 or 35-38 days of pregnancy.

Uterine endometrial cytology, biopsy, bacteriology, and serum C-reactive protein in clinically healthy diestrus bitches.

The aim of this work was to assess the agreement between endometrial cytology and uterine biopsy for the diagnosis of endometritis (END), the bacterial populations isolated from the vagina and uterus of bitches having END, and the measurement of C- reactive protein as a diagnostic tool for diagnosis of END in clinically healthy bitches. Fifty privately-owned intact, clinically healthy bitches, in diestrus, without a history of progestin administration, aged between 8 months and 6 years old and weighing between 5 and 28 kg were used in this study. Bitches were included in a program for breeding control at a municipal pet public shelter. Before ovariohysterectomy (OVX) samples for vaginal cytology and bacteriology, and blood samples were taken. After OVX endometrial cytology, bacteriological samples and biopsy were collected. Histologic examination was performed to confirm the uterine condition. Blood samples were centrifuged and stored at -20 °C until progesterone (P4) and C-reactive protein was measured. Samples for bacteriological culture were collected, and swabs were placed into Stuart's transport medium and transported to the laboratory. On histopathologic examination, the most common observation was END (27/50), followed by normal endometrium (NE; 18/50), cystic endometrial hyperplasia (CEH; 2/50), atrophy (2/50) and fibrosis with degeneration of the endometrial glands (1/50). Low degree of agreement was observed between results obtained by endometrial cytology samples and results obtained by biopsy in endometrial diagnosis (Kappa Coefficient: -0.19). In vaginal samples, ß-hemolytic Streptococcus, Staphylococcus spp., Escherichia coli, Proteus spp., Corynebacterium spp., and Klebsiella pneumoniae were the bacteria most often found. In uterine samples, only four samples from END showed bacterial grow. C-reactive protein frequency was higher in END (6/23, 23%) vs NE (0/16, 0%; Van der Waerden P-value = 0,0302). Our results support the hypothesis that END is a frequent finding in uterine biopsy and could be associated with subfertility and infertility in the bitch. A low degree of agreement was observed between the diagnostic results from the uterine biopsy and endometrial cytology. Bacteriology would not be recommended as a diagnostic tool because no bacteria highly associated with uterine diseases were isolated from bitches with END. Finally, the usefulness of C-reactive protein concentration as a marker for END in bitches could not be conclusively demonstrated.

Progesterone and ultrasonographic changes during aglepristone or cloprosternol treatment in queens at 21 to 22 or 35 to 38 days of pregnancy.

Progesterone (P4) is a requirement for pregnancy development. Previous reports observed a maximal value of serum P4 concentration on 21 days after the first mating after which it slowly declines throughout the rest of pregnancy. Ultrasound examination should be performed to ensure that pregnancy interruption is complete. Limited information is available on the ultrasonic appearance of conceptuses during pregnancy termination in cats The objective was to study serum P4 concentration and ultrasonographic changes during aglepristone (ALI) or cloprostenol (CLO) treatment and to evaluate the fertility after treatment. Two experiments (EXP) were carried out to accomplish this aim. Sixty queens, 12- to 36-month-old, were used. On Days 21 to 22 of pregnancy (EXP I) or 35 to 38 of pregnancy (EXP II), queens were divided into three groups (G). Queens in G1 received ALI (10 mg/kg, sc; EXP I, n = 10; EXP II, n = 10) for 2 consecutive days. Queens in G2 received CLO (5 µg/kg, sc; EXP I, n = 10; EXP II = 10) for 3 consecutive days. Queens in G3 received 1 mL of saline solution (PLA, sc; EXP I, n = 10; EXP II = 10). Blood samples were taken before treatment (Day 0) and every day during 10 days after the treatment to measure serum P4 concentrations. Likewise, after treatment, queens were monitored daily by ultrasonography for 10 days and weekly until the end of gestation to obtain gestational sacs measurements (GS), fetal measurements, and fetal biophysical profile. Data were analyzed by ANOVA. Serum P4 concentrations were significantly different on Day 6 (EXP I) and on Day 1 (EXP II) in ALI and CLO groups compared with PLA group (P < 0.05 and P < 0.01; respectively). The ultrasonographic monitoring during treatment allowed assessing changes in the GS and fetal measurements, embryo-fetal viability, and risk of pregnancy loss. In conclusion, the results from this study reported changes in serum P4 concentration and in ultrasonography measurements during pregnancy interruption with ALI or CLO treatment. Also it was observed that ALI and CLO are safe drugs and can preserve posttreatment queen fertility. Therefore, the results obtained in our work will be applied in feline reproduction practice.

Ultrasonographic and progesterone changes during Days 21 to 63 of pregnancy in queens.

Ultrasonography has been used to diagnose and monitor pregnancy. However, in the queen, most of ultrasonographic and endocrinological studies have been performed using small number of observations during limited periods of pregnancy. The aim of this study was to derive equations to predict the gestation age and parturition time using ultrasonographic embryo fetus measurements and serum progesterone (P4) concentration measurements. Mixed-breed queens (n = 16), aged between 24 and 36 months and weighing between 2 and 4 kg, were daily monitored by ultrasonography since 21 days after the first mating to parturition. Gestational sac (GS) was measured from longitudinal (length [LEN], anterior-posterior [ATP]) and transverse images (width [WID]), GS volume was calculated by the prolate ellipse formula, and GS diameter was calculated by orthogonal measurements. Fetal measurements included crown-rump length (CRL), head diameter (HD), and body diameter (BD). Gestational sac, fetal measurements, and serum P4 concentration were recorded and analyzed by ANOVA. Correlation and linear regression analyses were performed and equations were derived to estimate predicted values and 95% confidence interval for GS parameters and P4 concentrations from 21 to 63 days after the first mating and to estimate predicted values and 95% confidence interval for fetal parameters from Day 35 to 63 of gestation. The average concentrations of serum P4 concentration from Day 22 to 47 of gestation remained between 32.27 ± 4.25 and 16.25 ± 2.45 ng/mL. After that, a gradual decline occurs reaching a concentration of 2.99 ± 1.29 ng/mL 1 day before parturition. A positive and significant correlation between the ultrasonographic measurements (LEN, ATP, WID, GS volume and diameter, uterine wall thickness, CRL, HD, and BD) with number of days after the first mating was observed (P < 0.001). We observed a positive and significant correlation between GS measurements (LEN, ATP, and WID) and between fetal measurements (CRL, HD, and BD) and a negative and significant correlation between serum P4 concentration with GS (LEN, ATP, and WID), uterine wall thickness, and fetal (CRL, HD, and BD) measurements. In addition, there was a positive and significant correlation between serum P4 concentrations with days after the first mating to parturition. In conclusion, the equations derived from this study will be useful for pregnancy monitoring and for estimating pregnancy age in queens from Day 21 until parturition for animals with similar weight and age.

[Acute diarrhea: recent etiopathogenetic findings]. / Diarrea acuta: recenti acquisizioni eziopatogenetiche.

Acute diarrhoeas in children represent still now a very serious problem for Public Health either in developing or in industrialized Countries. In the last years great progress has been made in the aetiology and epidemiology of this disease. The most important results, which are worth recording, obtained in this field are: the detection of Rotavirus as a more common agent causing infectious enteritis in children; the "rediscover" of some pathogenic agents (Campylobacter and Yersinia) ignored since some years ago and now emerging; a renewed interest for "old" etiological agents (Giardia lamblia, that seems to be more frequent in industrialized Countries, and E. Coli, from which various pathogenetic mechanisms are now known). Either remarkable are the recent acquaintances on pathogenic mechanisms developed by the agents causing enteritis (production of cytotonic and cytotoxic toxins, invasion and adhesion of intestinal mucosa).

[Silver-Russell syndrome. Presentation of a case with autosomal dominant heredity]. / La sindrome di Silver-Russell. Presentazione di un caso ad ereditarietà autosomica dominante.

The Authors describe one case of Silver-Russel syndrome. An autosomic dominant pattern of heredity is presumed, because dysmorphic elements and low stature were noted in the mother and in other relatives.

[Kawasaki disease: between mystery and reality. A clinical contribution]. / La malattia di Kawasaki: fra mistero e realtà. Contributo clinico.

Kawasaki disease was described by T. Kawasaki in Japan in 1967 and since then numerous cases have been reported from all over the world. The Authors reports a review of the literature on the main epidemiologic, clinical, etiopathogenetic aspects of the Kawasaki disease, pointing up the present therapeutical trends and the importance of a correct follow-up. A case is reported of children with a particularly complete M.K.: she presented fever, mucosal hyperemia, lymph node swelling, cutaneous rash and desquamation of the fingers of hands and feet. Furthermore leukocytes platelets, alpha 2 globulins and ESR were raised.

[Pseudolithiasis caused by ceftriaxone in children: a case report]. / Pseudolitiasi da ceftriaxone in età pediatrica: descrizione di un caso clinico.

Recently, there are in literature some reports concerning the sonographic observation of transient gallbladder precipitates in children and adults during ceftriaxone treatment. The condition is defined as ceftriaxone-reversible "pseudolithiasis" and the gallbladder precipitates resolve spontaneously after cessation of therapy. We present a case of a 1 year old children treated with ceftriaxone who presented asymptomatic pseudolithiasis that resolved partially two weeks after cessation of treatment. The sonographic observation performed four months after cessation of therapy show a normally gallbladder, without precipitates or sludge.

Effect of melatonin implants on spermatogenesis in the domestic cat (Felis silvestris catus).

The aim of this study was to assess the efficacy of subcutaneous melatonin implants to temporarily and reversibly suppress spermatogenesis in male cats. Tomcats (n = 8) were housed in a conditioned room with alternating long and short 2-month photoperiod cycles to maintain sperm production and quality. Animals were randomly assigned to one of the two treatments. Four animals received a subcutaneous melatonin implant (MEL, 18 mg; Syntex, Argentina), whereas the other four received a subcutaneous placebo implant (PLA, 0 mg; Syntex). Semen samples were collected by electroejaculation every 14 days for 252 days. Sperm parameters were evaluated in all ejaculates, and data were analyzed by ANOVA. Melatonin-implanted cats significantly decreased their sperm quality in all the parameters studied compared with the control group (MEL vs. PLA; least squares means ± SEM; motility, 71.3 ± 3.4 vs. 82.1 ± 3.6; velocity, 3.4 ± 0.1 vs. 4.6 ± 0.1; total sperm count, 2.6 ± 2.2 vs. 19.4 ± 3.3; acrosome integrity, 48.7 ± 5.6 vs. 62.8 ± 5.6; plasma membrane integrity, 52.2 ± 4.7 vs. 72.9 ± 5.5; normal sperm morphology, 45.8 ± 3.3 vs. 63.7 ± 3.4; P < 0.05). Conversely, volume and serum testosterone concentrations were similar in both groups (volume, 0.15 ± 0.02; serum testosterone concentrations, 1.1 ± 0.1; CV 18.9%; P > 0.05). At 91 ± 7 days after implant insertion, sperm motility decreased 38.5%, velocity 26.5%, total sperm count 82%, acrosome integrity 22%, plasma membrane integrity 30%, and normal sperm morphology decreased 32% of preimplant values. This effect was present until 120 ± 15 days after implant insertion. After that, seminal parameters started to increase and reached preimplant values at about 140 ± 7 days after implant insertion. Nevertheless, treated animals conserved the capacity to produce semen during the treatment period. In conclusion, a single subcutaneous melatonin implant effectively and reversibly reduced sperm production and quality in male domestic cats for approximately 120 ± 15 days without clinically detectable adverse effects.