Treatment of climacteric symptoms with an ammonium succinate-based dietary supplement: a randomized, double-blind, placebo-controlled trial.

Peri- and postmenopausal women commonly suffer from climacteric symptoms. We evaluated the effectiveness and safety of dietary supplement Amberen to relieve vasomotor and psychosomatic symptoms during the course of a 3-month, randomized, double-blind, placebo-controlled study. General clinical assessment, evaluation using the Greene climacteric test and Spielberger-Hanin test, determination of plasma levels of gonadotropins, estradiol, leptin and apolipoproteins were used to evaluate 42-60-year-old women with vasomotor and psychosomatic menopausal symptoms. One hundred and twenty-five women were enrolled in the study and randomized between two groups. Based on the Greene test results, there was a statistically significant improvement (р < 0.05) in 13 out of 21 menopausal symptoms in women who took Amberen. During the course and by the end of the study, patients showed statistically significant changes in the levels of estradiol, gonadotropins and leptin, and decreases in body weight and waist circumference. Spielberger-Hanin test showed that Amberen stabilizes patients' psychological state with a statistically significant decrease in anxiety, increased stress resistance and improved adaptability. Comparative analysis of the vital signs measurements, blood tests and urinalysis did not show any negative effects of Amberen on the patients. Our findings indicate that Amberen can be considered a method of choice to relief mild/moderate climacteric symptoms.

[The role of innate immunity system in the course of adenomyosis].

The investigation of leukocytic elastase (LE) and alpha1-proteinase inhibitor (alpha1-PI) from patients with different stage adenomyosis and in control group was found activation innate immunity system in all the patients with adenomyosis. The degree of LE activity is a prevalence rate of adenomyosis. The degree of alpha1-PI activity is correlated with antiproteolytic potential that blocks the effects shown by LE. It can lead the prognose of disease and timely treatment.

[Immunohistochemical examinations of dyskeratosis and exocervical neoplasia in gynecological pathology]. / Immunogistokhimicheskie issledovaniia diskeratoza i neoplasticheskikh izmenenii ékzotserviksa pri ginekologicheskoi patologii.

An immunohistochemical study was carried out with monoclonal antibodies to estrogen receptors (Ers), Ki-67, BCL-2, P53 and human papillomaviruses (HPV) of normal cervix (7 cases), cervical leukoplakia (5 cases), CIN I (7 cases) and CIN II-III (6 cases). Correlations were also investigated between hyperestrogenemia and molecular changes in the exocervix. About two thirds of patients with leukoplakia and precancerous changes had clinical signs of hyperestrogenemia which led to proliferation of estrogen-sensitive parabasal cells. Due to neoplastic epithelial transformation the number of cells with Ki-67, P53 and HPV positive reactions in the nuclei increased, while Ers and BCL-2 expression decreased. Because active proliferative parabasal cells are needed for HPV replication we consider hyperestrogenemia as a factor stimulating development of neoplastic changes in the uterine cervix.

[Comparison of free and membrane-bound polyribosomes of human placenta in different periods of pregnancy]. / Cravnitel'noe izuchenie svobodnykh i membranosviazannykh poliribosom platsenty cheloveka v razlichnye sroki beremonnosti.

The amount and sedimentation properties of free and membrane-bound polyribosomes of human placenta were studied at different stages of normal pregnancy. The amount of total ribosomes decreases considerably with an increase in the time of pregnancy, while a portion of membrane-bound polyribosomes tends to increase. It is shown that sedimentation patterns of polyribosomes of the both types change during development of pregnancy. These alterations may be associated with changes in a set of proteins which are produced by the placenta during its ripening.

[Protein-synthesizing apparatus of the rat liver and placenta during damage of the biomembrane by tetrachloromethane and the administration of methylxanthines]. / Beloksinteziruiushchii apparat pecheni i platsenty krys pri povrezhdenii biomembran tetrakhlormetanom i vvedenii metilksantinov.

Treatment of rats with tetrachloromethane was accompanied by a decrease in total ribosomal material, an increase in free, unbound with membranes, polyribosomal fraction and marked decrease in 14C-leucine and 3H-orotate incorporation into proteins and RNA of liver and placenta cells. Administration of methylxanthines--euphylline and trental protected the protein-synthesizing apparatus of hepatocytes and placenta from the damaging effects of tetrachloromethane. The role of microsomal monooxygenase system in tetrachloromethane activation and in impairment of cAMP-dependent regulation of translation process under conditions of xenobiotic-induced cell injury are discussed.

[Effect of antibiotic therapy on embryotropic antibody level in women]. / Vliianie antibiotikoterapii na coderzhanie émbriotropnykh antitel u zhenshchin.

Embriotropic antibodies (e-Ab) serum contents were analysed in 29 serum samples obtained from women suffering with primary lues. Before treatment the content of e-Ab against MBP, S100, ACBP14/18, and MP65 were markedly elevated in 28 samples. Two injections of Retarpen (2.5 mln Units with one week interval) were enough for effective treatment of lues. At the same time treatment caused deep and reversible inhibition of e-Ab production. One or two months later the levels of e-Ab returned to normal values in 21% of women, in 52% patients the hypoproduction, and in 27% the hyperproduction of e-Ab were noted. Deviations of e-Ab production after effective retarpen treatment suggest the existence of additional reasons which may influence e-Ab production, and necessity of additional diagnostic and curative measures for such women during pregravidal period.

[Experience in using perftoran in combined treatment of placental insufficiency in severe gestosis]. / Opyt primeneniia perftorana v kompleksnom lechenii platsentarnoi nedostatochnosti pri tiazhelykh gestozakh.

Twenty-two women with severe gestosis were examined during weeks 27-38. They were divided into 2 groups with different intensive care protocols: 1) osmooncotherapy and 2) 3-4 infusions of perfluorane in a dose of 3-4 ml/kg every other day. Addition of perfluorane to combined therapy promoted a more rapid stabilization of hemodynamics, metabolism, and helped prolong the pregnancy to 37-38 weeks.

[Reconstructive plastic operations in ptosis and prolapse of the genitalia. The choice of the technic and the prevention of complications]. / Rekonstruktivnye plasticheskie operatsii pri opushcheniiakh i vypadeniiakh polovykh organov. Vybor tekhniki i profilaktika oslozhnenii.

The principal causes of recurring prolapses are inadequate choice of surgical technique and unqualified performance of the operation proper, as well as the absence of expert evaluation of the performance capacity of this patient population. The authors suggest pathogenetically, anatomically, and functionally validated methods of reconstructive surgery permitting restoration of the position and function of the genitals and the adjacent organs and helping preserve the generative function of fertile women. Approaches to prevention of recurrences are outlined.

Study of Candida albicans strains isolated from women with various forms of vaginal candidiasis.

Adhesive activity of Candida albicans towards vaginal epitheliocytes is hormone-dependent. Two types of C. albicans adhesins sensitive to polyphenyloxidase and asparaginase were detected. Laser irradiation nonspecifically modulated both adhesin types. Population relationships between fungi and lactobacilli in patients with vaginal candidal infection and in C. albicans carriers were studied. Genodiagnostic method for identification of C. albicans and morphogenesis-associated genes of these yeast-like fungi was approved.