[Biological preparation based on Lactobacillus acidophilus, a new agent in the early treatment of combined radiation-thermal injuries]. / Biopreparat na osnove Lactobacillus acidophilus--novoe sredstvo rannego lecheniia kombinirovannykh radiatsionno-termicheskikh porazhenii.

Male mice F1 (CBA x C57BL6) were used for experiments. Animals were exposed to 7 Gy gamma-radiation and additionally inflicted to full-thickness thermal burn 10% body surface. As it has been revealed, single subcutaneous injection of the created biopreparation based on inactivated lactobacillus microbic biomass increased mice survival with combined injury from 23% to 73%. Therapeutic efficacy of this remedy did not correlate with postradiation damages of the hemopoietic system. Injection of killed L. acidophilus mixture strikingly averted development of the intestine autoinfection. Possible mechanisms of the benefit therapeutic action of the new preparation are discussed.

[Antagonistic interrelationships of Bifidobacterium bifidum i Proteus vulgaris in vitro in the digestive tract of gnotobiotic chicks]. / Antagonisticheskie vzaimootnosheniia Bifidobacterium bifidum i Proteus vulgaris in vitro v pishchevaritel'nom trakte tsypliat-gnotobiontov.

The antagonistic relations between Bacterium bifidum, strain I/850 phi, and Proteus vulgaris, strain F-30, were studied. These organisms, when introduced together in equal doses into the digestive tract of gnotobiotic chickens in a single administration, were shown to create certain ecological correlations in various organs with the prevalence of bifidobacteria which exerted no negative influence on Proteus vulgaris. The additional daily administration of bifidobacteria for 3 days running in doses 1000 times as great as the initial dose, the content of both dibifobacteria and Proteus vulgaris in the intestine being at that time at its maximum, resulted in the suppression of the growth of Proteus vulgaris. Our findings indicate that the influence of the pH of the medium should be considered in order to obtain the evidence of significantly pronounced antagonistic relations between the two organisms in vitro.

[Intestinal microflora of monkeys, normal and in pathology, and its correction by preparations of live bacteria from human biocenosis (coli bacteria, bifikol and bifidum bacterin)]. / Mikroflora kishechnika obez'ian v norme i pri patologii i ee korrektsiia preparatami iz zhivykh bakterii obligatnogo biotsenoza cheloveka (koli-bakterii, bifikol, bifidum-bakterin).

The study of the qualitative and quantitative composition of intestinal microflora in 15 anthropoid apes, 60 lower primates and 72 monkeys with clinically pronounced dysbacteriosis was made, which revealed the prevalence of microorganisms belonging to the genus Proteus, a decrease in the biological activity of normal Escherichia coli and in the content of bifido- and lactobacteria. The treatment of 39 rhesus and pig-tailed macaques with the preparations of live bacteria occurring in normal human microflora led to clinical convalescence and the normalization of the bacteriocenosis, while in 33 control animals no positive shifts in clinical and bacteriological data were observed. The essential similarity of the composition of intestinal microflora in higher and lower primates and in man makes it possible to use these animals as models for testing the effectiveness of new biological preparations and determines the expediency of using bacterial therapy for the treatment of monkeys at the period of acclimatization.

[Interaction between Bifidobacterium bifidum, Proteus vulgaris, and Klebsiella pneumoniae 204 in the gastrointestinal tract of gnotobiotic chicks]. / Vzaimodeistvie mezhdu Bifidobacterium bifidum, Proetus vulgaris i Klebsiella pneumoniae 204 v pishchevaritel'nom trakte tsypliat-gnotobiontov.

Experiments on gnotobiont chickens indicated that the strains B. bifidum 1/85 phi, P. vulgaris F-30 and K. pneumoniae 204, when introduced simultaneously into the gastrointestinal tract in a single administration, proliferate there with the pronounced predominance of bifidobacteria. 6 additional administrations of B. bifidum 1/85 phi culture resulted in the complete suppression of microorganisms belonging to the genera Rroteus and Klebsiella as early as 10 days after the introduction of bifidobacteria. These data suggest that it is necessary to use B. bifidum 1/85 phi in cases of intestinal dysbacteriosis characterized by the predominance of microorganisms belonging to the genera Proteus and Klebsiella.

[Mechanism of antagonistic activity of lactobacilli]. / K mekhanizmu antagonisticheskoi aktivnosti laktobatsill.

Different species of lactobacilli, when grown together with salmonellae and shigellae, have been found to inhibit their growth and proliferation. The supernatants of the culture fluids of 8 species of lactobacilli have been shown to contain natural metabolites, other than lactic acid, with a wide spectrum of antibacterial activity with regard to different opportunistic aerobic and anaerobic microorganisms. The relationship between the antagonistic activity and the presence of plasmid DNA in a number of the most active antagonistic lactobacilli is discussed.

[The adhesive properties of bacteria of intestinal origin]. / Adgezivnye svoistva bakterii kishechnogo proiskhozhdeniia.

Differences between strains of nonpathogenic Escherichia and lactobacilli, as well as some pathogenic bacteria of enteric origin (Escherichia, Shigella, Campylobacter), in their capacity to adhesion to rat enteric and colonic cells have been shown in vitro. The strains under study have been found to possess more pronounced adhesiveness with respect to colonic cells, which is indicative of their higher receptive capacity in comparison with enteric cells. In the absence of normal microflora lactobacilli and Escherichia exhibit increased adhesiveness with respect to enteric cells. Escherichia enterotoxigenic strains, Yersinia enterocolitica and Salmonella typhimurium virulent strains, Campylobacter jejuni clinical isolates possess more pronounced capacity for adhesion to enteric cells of Peyer's plaques than to other types of epithelial cells, which may be of importance in the pathogenesis of these infections.

[Effect of bificol on the intestinal microflora of chronic colitis patients working in antiboitic prodiction]. / Vliianie bifikola na mikrofloru kishechnika bol'nykh knronicheskim kolitom u usloviiakh proizvodstva antibiotikov

Data on the use of bificol, a new Soviet preparation, and its effect on the intestine microflora of patients with chronic colitis occupied in production of penicillin are presented. It was shown that by the 28th day of the preparation use the level of the intestine bacteria in the patients' intestine reliably increased. The number of immobile strains decreased from 67.6 to 36.6 per cent. Bifidoflora normalized by the 14th day of the treatment. Some clinical inprovement, i.e. stool normalization, lessening of the stomach pain, increased appetite were observed by the 4th--5th day of the treatment with bificol. On the basis of the microbiological and clinical data it was shown that treatment of the patients with chronic colitis in antibiotic production should continue for at least 28 days and in individual cases for longer periods of time. It is recommended to use the preparation in 10 doses a day divided into 2 parts.