ABSTRACT
ω-Aminotransferase (ω-AT) is an important class of enzymes for the synthesis of chiral amines or ß-amino acids. Family profile analysis was applied to screen putative ω-ATs from Mesorhizobium loti MAFF303099, a nitrogen fixation bacterium that has a larger number of ATs than other microorganisms. By family profile analysis, we selected 10 putative ω-ATs according to E-value. The functions of the putative ω-ATs were investigated by examining activities towards amines and/or ß-amino acids. 10 putative proteins were found to have ω-AT activity with narrow or broad substrate specificity. Structure analysis using crystal structure of mll7127 and homology models of mll1632 and mll3663 indicated that the structures of active sites of the enzymes were very similar and highly conserved, but their substrate specificities appeared to be determined by residues positioned at the entrance region of the active site binding pockets.
Subject(s)
Amino Acids/chemistry , Bacterial Proteins/chemistry , Genome, Bacterial , Mesorhizobium/chemistry , Transaminases/chemistry , Amino Acids/genetics , Amino Acids/metabolism , Bacterial Proteins/genetics , Bacterial Proteins/metabolism , Catalytic Domain , Chromosome Mapping , Computational Biology , Crystallography, X-Ray , Isoenzymes/chemistry , Isoenzymes/genetics , Isoenzymes/metabolism , Mesorhizobium/enzymology , Mesorhizobium/genetics , Models, Molecular , Multigene Family , Mutagenesis, Site-Directed , Nitrogen Fixation/genetics , Recombinant Proteins/chemistry , Recombinant Proteins/genetics , Recombinant Proteins/metabolism , Structural Homology, Protein , Substrate Specificity , Transaminases/genetics , Transaminases/metabolismABSTRACT
Adhesion is a primary challenge following surgery, and the anti-adhesive effect of methylene blue (MB) has been investigated. This systematic review and meta-analysis aimed to evaluate the effect of MB on postoperative adhesions in experimental studies. We initially searched OVID-MEDLINE, EMBASE, and Google Scholar in February 2021, and then in May 2021. The anti-adhesive efficacy of MB was compared with that of the control (either placebo or nothing) after the surgical procedure. The primary and secondary outcomes were the macroscopic and microscopic adhesion scores, respectively. Traditional meta-analysis, meta-regression, and trial sequential analysis (TSA) were performed to analyze the retrieved outcomes. We included 13 experimental studies of 367 rats (200 rats received MB and 167 rats received placebo or nothing). The macroscopic adhesion scores were significantly lower in the MB-administered group than in the control group (standardized mean difference, 2.313; 95% confidence interval, 1.104 to3.523; I2 = 94.0%, Tau = 2.059). Meta-regression analysis showed that macroscopic adhesion tended to decrease with an increase in MB dose. TSA demonstrated that the cumulative Z curve crossed both the conventional test and trial sequential monitoring boundary for the macroscopic adhesion score. MB had a beneficial effect on intraperitoneal adhesion following laparotomy, and adhesions decreased with increase in dose.
Subject(s)
Laparotomy , Methylene Blue , Animals , Methylene Blue/pharmacology , Rats , Tissue Adhesions/prevention & controlABSTRACT
This study reports the effect of processing, fermentation, and aging treatment on the content and profile of 43 phenolic compounds in soybean seeds, soy curd (tofu), and soy paste (ChungGukJang, CGJ). Mean content of phenolic compounds was ranked as soybean seed=CGJ aged for 3days (CGJ-3D)=CGJ aged for 6days (CGJ-6D)>tofu (P<0.0001). Low percent recovery (47.1%) of phenolic compounds in tofu was due to heating (boiling), leaching in water, filtering, coagulation, and whey exclusion during tofu making. Aging period did not affect the mean contents of 43 phenolic compounds in the CGJ, whereas it affected the phenolic acids contents in the CGJ (P<0.01). Benzoic, ferulic, chlorogenic, gentisic, protocatechuic, or ß-Resorculic acid was major phenolic compounds in soybean seeds, tofu, CGJ-3D, or CGJ-6D. Especially, the CGJ-3D contained large amounts of isoflavone aglucons and phenolic acids compared to soybean seeds or tofu.
ABSTRACT
BACKGROUND: Nasotracheal intubation is generally performed for intraoral surgery. CASE: A 34-year-old female patient who underwent orthognathic surgery exhibited repeated endotracheal tube cuff tears during nasotracheal intubation. After intubation, leaks developed, and torn endotracheal cuff was observed in the removed endotracheal tube. Subsequently, re-intubation through the same nasal cavity was performed immediately, but leakage from the torn endotracheal tube cuff was re-observed. A leakage test of the extubated tube revealed air bubbles and leaks near the tube cuff due to the tear. Nasotracheal intubation was performed through the other nasal cavity, and there were no leakage findings or abnormalities. During the course of the surgery, the surgeon noticed that the orthodontic micro-implant deposited in the mid-tube cavity was exposed to the nasal cavity. CONCLUSIONS: We aimed to emphasize caution and discuss the possibility that orthodontic micro-implants that are not confirmed during preoperative evaluation may cause repeated endotracheal tube cuff tears.
ABSTRACT
Essential oil obtained from the leaves of Pittosporum tobira was extracted and its chemical composition and larvicidal effects were studied. Analyses were conducted by gas chromatography and mass spectroscopy (GC-MS) to determine the primary constituents of the essential oil of P. tobira. The yield of P. tobira essential oil (PTEO) was 0.1%, and GC-MS analysis identified its major constituents as undecane (31.11%), 4-methyl-1,3-pentadiene (11.34%), (1,3-dimethyl-2-butenyl)benzene (5.45%), and L-limonene (14.08%). The essential oil had a significant toxic effect against early fourth-stage larvae of Aedes aegypti (L.), with an LC(50) value of 58.92 ppm and an LC(90) value of 111.31 ppm. Finally, the LC(50) and LC(90) values of L-limonene were 39.7 ppm and 78.11 ppm. These results could be useful for seeking newer, safer, and more effective natural larvicidal agents against A. aegypti.
Subject(s)
Aedes/drug effects , Larva/drug effects , Oils, Volatile/pharmacology , Plant Oils/pharmacology , Rosales/chemistry , Animals , Gas Chromatography-Mass Spectrometry , Insecticides/isolation & purification , Mosquito Control/methods , Oils, Volatile/analysis , Oils, Volatile/isolation & purification , Plant Leaves/chemistry , Plant Oils/analysis , Plant Oils/isolation & purificationABSTRACT
To examine the neuroprotective effects of Caesalpinia sappan L., we tested its protection against the glutamate-induced neurotoxicity in primary cortical cultured neurons. We found that an aqueous extract of this medicinal plant exhibited significant protection against glutamate-induced toxicity in primary cultured rat cortical cells. In order to clarify the neuroprotective mechanism(s) of this observed effect, isolation was performed to seek and identify active fractions and components. By such fractionation, two known compounds - sappanchalcone and 3'-deoxy-4-O-methylepisappanol - were isolated from the methanol extracts from the air-dried and chipped C. sappan. Among these two compounds, 3'-deoxy-4-O-methylepisappanol exhibited significant neuroprotective activities against glutamate-induced toxicity, exhibiting cell viability of about 50%, at concentrations ranging from 0.1 microM to 10 microM. Therefore, the neuroprotective effect of C. sappan might be due to the inhibition of glutamate-induced toxicity by the protosappanin derivative it contains.
Subject(s)
Caesalpinia/chemistry , Glutamic Acid/toxicity , Neurons/drug effects , Neuroprotective Agents/pharmacology , Phenols/pharmacology , Plant Extracts/pharmacology , Animals , Cells, Cultured , Cerebral Cortex/cytology , Chalcones/isolation & purification , Chalcones/pharmacology , Molecular Structure , Neuroprotective Agents/isolation & purification , Phenols/isolation & purification , Rats , Rats, Sprague-DawleyABSTRACT
The whole plants of Carpesium divaricatum are used in traditional medicine as an antipyretic, analgesic and vermifugic, including a topical application for sores and inflammation. In a previous paper, the isolation and structural elucidation of isolated ineupatorolides A from methanol extracts of C. rosulatum (Compositae) were reported. The present paper deals with the isolation and identification of antiplasmodial compounds from C. divaricatum in Carpesium spp. Five compounds, 1 beta,6 alpha-dihydroxy-4(15)-eudesmene [1], beta-dictyopterol [2], 2-isopropenyl-6-acetyl-8-methoxy-1,3-benzodioxin-4-one [3], (3E,6E,10E,14E,18E)-2,6,10,15,19,23-hexamethyl-3,6,10,14,18,22-tetracosahexaen-2-ol [4] and neophytadiene [5], were isolated from the whole plants of C. divaricatum. The in vitro activity against P. falciparum strain D10 was assessed using the parasite lactate dehydrogenase assay method. The main antiplasmodial principle, 2-isopropenyl-6-acetyl-8-methoxy-1,3-benzodioxin-4-one [3], has been isolated from C. divaricatum for the first time. Compound 3 exhibited an antiplasmodial activity with IC(50) values of 2.3 +/- 0.3 microm. This is the first report on the antiplasmodial activity of the compounds from C. divaricatum.
Subject(s)
Antimalarials/isolation & purification , Asteraceae/chemistry , Dioxins/isolation & purification , Plant Extracts/chemistry , Plasmodium falciparum/drug effects , Antimalarials/pharmacology , Cell Line, Tumor , Dioxins/pharmacology , Humans , Molecular StructureABSTRACT
BACKGROUND: Petroleum ether extracts of Viola websteri Hemsl (Violaceae) were reported to have anti-plasmodial activity against Plasmodium falciparum in vitro, with this activity being largely attributable to 6-(8'Z-pentadecenyl)-salicylic acid (6-SA). METHODS: The schizontocidal activity of 6-SA on early Plasmodium berghei infections was evaluated in a four-day test. The possible 'repository' activity of 6-SA was assessed using the method described by Peters. The median lethal dose (LD50) of 6-SA, when given intraperitoneally, was also determined using uninfected ICR mice and the method of Lorke. RESULTS: In the present study, 6-SA was found to have anti-malarial activity in vivo, when tested against P. berghei in mice. 6-SA at 5, 10 and 25 mg/kg x day exhibited a significant blood schizontocidal activity in four-day early infections, repository evaluations and established infections with a significant mean survival time comparable to that of the standard drug, chloroquine (5 mg/kg.day). CONCLUSION: 6-SA possesses a moderate anti-malarial activity that could be exploited for malaria therapy.
Subject(s)
Antimalarials/pharmacology , Plant Extracts/pharmacology , Plasmodium berghei/drug effects , Salicylic Acid/pharmacology , Viola/chemistry , Animals , Dose-Response Relationship, Drug , Lethal Dose 50 , Malaria/drug therapy , Mice , Mice, Inbred ICR , Phytotherapy , Plant Extracts/isolation & purification , Salicylic Acid/isolation & purificationABSTRACT
PURPOSE: Mechanical stapler is regarded as a good alternative to the hand sewing technique, when used in gastric reconstruction. The circular stapling method has been widely applied to gastrectomy (open orlaparoscopic), for gastric cancer. We illustrated and compared the hand-sutured method to the circular stapling method, for Billroth-II, in patients who underwent laparoscopy assisted distal gastrectomy for gastric cancer. MATERIALS AND METHODS: Between April 2009 and May 2011, 60 patients who underwent laparoscopy assisted distal gastrectomy, with Billroth-II, were enrolled. Hand-sutured Billroth-II was performed in 40 patients (manual group) and circular stapler Billroth-II was performed in 20 patients (stapler group). Clinicopathological features and post-operative outcomes were evaluated and compared between the two groups. RESULTS: Nosignificant differences were observed in clinicopathologic parameters and post-operative outcomes, except in the operation times. Operation times and anastomosis times were significantly shorter in the stapler group (P=0.004 and P<0.001). CONCLUSIONS: Compared to the hand-sutured method, the circular stapling method can be applied safely and more efficiently, when performing Billroth-II anastomosis, after laparoscopy assisted distal gastrectomy in patients with gastric cancer.
ABSTRACT
Screening of bacterial whole cells was performed for regioselective hydroxylation of daidzein and genistein. Among the strains examined, Streptomyces avermitilis MA-4680 showed high ortho-dihydroxylation activity to produce 3',4',7-trihydroxyisoflavone and 3',4',5,7-tetrahydroxyisoflavone from daidzein (4',7-dihydroxyisoflavone) and genistein (4',5,7-trihydroxyisoflavone), respectively. Using 100 mg cells (wet wt.) and 1% (v/v) Triton X100 in 1 ml of total reaction volume, where 100 microl of the substrate solution (0.5 mM in 10% (v/v) mixed solvent of DMSO:MeOH = 3:7) was added to 900 microl of potassium phosphate buffer (100 mM, pH 7.2), a 16% molar conversion yield of 3',4',7-trihydroxyisoflavone was obtained from 0.5 mM daidzein after 24 h of reaction time at 28 degrees C and 200 rpm. Ketoconazole significantly (ca. 90%) inhibited the ortho-hydroxylation activity of daidzein, suggesting that cytochrome P450 enzymes putatively play roles in regiospecific daidzein hydroxylation. The analysis of the reaction products was determined by gas chromatography/mass spectrometry (GC/MS) and (1)H NMR.
Subject(s)
Isoflavones/metabolism , Streptomyces/metabolism , Biotransformation , Cytochrome P-450 Enzyme Inhibitors , Detergents/chemistry , Enzyme Inhibitors/pharmacology , Gas Chromatography-Mass Spectrometry , Hydroxylation , Isoflavones/pharmacokinetics , Kinetics , Magnetic Resonance SpectroscopyABSTRACT
A study was conducted to determine the content of phenolic compounds and the antioxidative activity of five edible and five medicinal mushrooms commonly cultivated in Korea. Phenolic compounds were analyzed using high performance liquid chromatography, and antioxidant activity was evaluated by 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity and superoxide dismutase activity. A total of 28 phenolic compounds were detected in the mushrooms studied. The average total concentration of phenolic compounds was 326 microg/g, the average being of 174 microg/g in edible mushrooms and 477 microg/g in medicinal mushrooms. The average total flavonoids concentration was 49 microg/g, with averages of 22 and 76 microg/g in edible and medicinal mushrooms, respectively. The DPPH radical scavenging activities ranged between 15 (Pleurotus eryngii) and 70% (Ganoderma lucidum) when reaction time was for 1 min. When reaction time was 30 min, the values ranged between 5 (Pleurotus eryngii) and 78% (Agaricus bisporus). The SOD activity averaged 28% among the 10 mushroom species, averages for edible and medicinal mushrooms being comparable. DPPH activities was significantly correlated (p < 0.01) with total content of phenolic compounds in edible mushrooms, while in medicinal mushrooms there was a significant correlation (p < 0.01) between SOD activity and total concentration of phenolic compounds. Numerous significant positive correlations were observed between phenolic compounds detected and antioxidative potential.