Long Non-Coding RNA (lncRNA) Metastasis-Associated Lung Adenocarcinoma Transcript 1 (MALAT1) Promotes Cell Proliferation and Migration by Regulating miR-143-3p and MAGE Family Member A9 (MAGEA9) in Oral Squamous Cell Carcinoma.

BACKGROUND lncRNA MALAT1 is one of the most widely studied lncRNAs associated with various human cancers. The present study explored the functions and potential regulatory mechanisms of MALAT1 in oral squamous cell carcinoma (OSCC). MATERIAL AND METHODS We assessed levels of MALAT1, miR-143-3p, and MAGEA9 expression in OSCC tissues and cell lines by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot assay. Proliferation and migration of CAL-27 cells were detected via CCK-8 and transwell assays, respectively. To study the relationships among MALAT1, miR-143-3p, and MAGEA9, we performed dual-luciferase assay and assessed the results using Spearman correlation analysis. RESULTS QRT-PCR results showed that MALAT1 and MAGEA9 were expressed at higher levels and miR-143-3p was expressed at lower levels in OSCC tissues. Dramatic suppression of cell proliferation and migration abilities were caused by MALAT1 knockdown or miR-143-3p overexpression in CAL-27 cells. MALAT1 directly interacted with and negatively regulated miR-143-3p. Moreover, MAGEA9 was validated as a miR-143-3p target gene and was found to be negatively regulated by it. MALAT1 knockdown suppressed MAGEA9 protein expression and had the same effect as MAGEA9 knockdown. Additionally, MAGEA9 knockdown inhibited CAL-27 cell proliferation and migration abilities. Finally, in OSCC tissues, MALAT1 and miR-143-3p expression were negatively correlated and MALAT1 was positively correlated with MAGEA9 expression, while an inverse correlation between MAGEA9 and miR-143-3p expression was observed. CONCLUSIONS Taken together, our results suggest that MALAT1 functions as a competing endogenous RNA (ceRNA) in promoting OSCC cell proliferation and migration abilities through the miR-143-3p/MAGEA9 axis, thus providing new therapeutic targets for treatment of OSCC.

Dual Antiplatelet Therapy after Intravenous Thrombolysis for Acute Minor Ischemic Stroke.

OBJECTIVE: To verify the efficacy and safety of dual antiplatelet therapy after intravenous thrombolysis for acute minor ischemic stroke (AMIS). METHODS: AMIS patients who received recombinant tissue plasminogen activator (rt-PA) intravenous thrombolysis from January to October 2018 were retrospectively analyzed and divided into the aspirin (ASP) and ASP + clopidogrel (ASP-CLO) groups based on the type of antiplatelet therapy to compare the rates of good clinical outcome, symptomatic intracranial hemorrhage (SICH) after thrombolysis, and mortality in 90 days. RESULTS: A total of 207 patients were included (group ASP, 105 patients; group ASP-CLO, 102 patients). There was no significant difference in the baseline clinical data between the 2 groups. The -90-day modified Rankin scale scores (66.7 vs. 82.4%, p = 0.009) showed a statistically significant difference, but SICH (1.0 vs. 1.0%, p = 0.917) and 90-day mortality (1.9 vs. 1.0%, p = 0.585) showed no significant difference between the 2 groups. CONCLUSIONS: Short-term (21 days) dual antiplatelet therapy after rt-PA intravenous thrombolysis for AMIS can improve the prognosis, reduce the risk of stroke recurrence, without increasing the risk of bleeding and mortality.

High-efficient carbon dioxide-to-formic acid conversion on bimetallic PbIn alloy catalysts with tuned composition and morphology.

CO2 electroreduction to value-added chemicals and fuels has gained increasing attention; however, there are only a few catalysts with high performance under mild conditions that can be used in this technique. In this study, single metal Pb, In and bimetallic PbIn catalysts for aqueous CO2 electroreduction were prepared using a facile 3-step process including PbIn granulation by reducing Pb(NO3)2/In(NO3)3 aqueous solution with NaBH4, calcination in air, and in situ electroreduction. The bimetallic PbIn catalysts had better catalytic performance on CO2 electroreduction than single metal catalysts. The bimetallic Pb7In3 catalyst (atomic ratios of Pb and In is 7:3) presented the highest formic acid faradaic efficiency of 91.6% at -1.26 V vs reversible hydrogen electrode in a 0.5 M CO2-saturated KHCO3 aqueous solution, which was 13% and 9.7% higher than that of single Pb and In catalysts, respectively. Moreover, the catalyst remained active after 10 h of continuous CO2 electrolysis with a stale current density of -17 mA cm-2. The experimental results showed that the excellent catalytic performance of Pb7In3 catalyst may stem from its higher electrochemical active surface area, lower charge-transfer resistance and the synergistic effect of Pb and In in the catalyst. The presented bimetallic PbIn catalysts may have a wide of application prospect, and they may be synthesized from heavy metals in industrial wastewaters.

Electronic structural modulation of bismuth catalysts induced by sulfur and oxygen co-doping for promoting CO2 electroreduction.

Carbon dioxide electroreduction into green fuels and value-added chemicals is an attractive method for the utilization of renewable energy to mitigate global warming. High-efficiency catalysts are necessary for mild and efficient conversion via this process. Metal sulfides have shown potential applications in energy conversion and storage thanks to the synergistic effect induced by sulfur and metal atoms. Here, we reported a novel sulfur and oxygen co-doped Bi catalyst derived from bismuth sulfide (Bi2S3) for the selective CO2 electrochemical reduction to formate. As a result, 89.7% formate faradaic efficiency at -1.09 V versus the reversible hydrogen electrode was obtained, and was higher than that obtained by using a pure Bi electrode (84.5%). The characterization and electrochemistry results indicated that co-doping with sulfur and oxygen atoms improved the surface electronic structure of the material, which further affected the adsorption of CO2 and the formation of reaction intermediates. This study provides a novel catalyst for CO2-to-formate conversion and also reveals changes in the metal sulfide structure and composition before and after CO2 electroreduction, which are thought to be the key to enhancing the catalytic performance of bismuth sulfide. A useful basis for the design of metal sulfide-based catalysts is also provided.

Perceived stress and life satisfaction: social network service use as a moderator.

Social Network Service (SNS) has become a buzzword in recent media coverage with the development of the second generation of Web-based communities. In China, SNS has played an increasingly important role in its users' daily lives, especially among students. With a sample of 471 college students, we tested the direct relationship between perceived stress and life satisfaction using a regression analysis. Moreover, we found SNS use could buffer the negative effect of perceived stress. This study has practical implications on Internet users' SNS use.

[Ryanodine downregulates the expression of p-eNOS (Thr495) and improves the functions of rapamycin treated endothelial outgrowth cells].

OBJECTIVE: To observe the effects of ryanodine on rapamycin treated endothelial outgrowth cells (EOCs). METHODS: The mononuclear cells were harvested from umbilical cord blood by Ficoll density gradient centrifugation, then induced into EOCs and expanded in vitro. The endothelial characteristics of EOCs were identified by immunostaining and fluorescent staining. The EOCs were pretreated with or without ryanodine (10 µmol/L) for 1 h, and then treated with or without rapamycin (10 nmol/L) for 24 h. Proliferation was evaluated by CCK8 and migration was measured by Transwell. The protein expression of EOCs was evaluated by immunobloting technique with total eNOS antibody and phospho-eNOS (Thr495) antibody. RESULTS: Compared with control group, the proliferation and migration capacities of EOCs were significantly reduced while the phosphorylation of eNOS (Thr495) protein was significantly upregulated in rapamycin group (P < 0.05), expression of total eNOS was not affected by rapamycin (P > 0.05). Compared with rapamycin group, the proliferation and migration capacities of EOCs were significantly increased and the phosphorylation of eNOS (Thr495) protein was significantly downregulated in ryanodine + rapamycin group (P < 0.05). The proliferation and migration capacities, the phosphorylation of eNOS (Thr495) protein and the expression of total eNOS were not affected by ryanodine alone (P > 0.05). CONCLUSIONS: Rapamycin reduced proliferation and migration capacities while upregulated the phosphorylation of eNOS (Thr495) protein of EOCs and these effects could be partly reversed by cotreatment with ryanodine.

lncRNA SNHG5 promotes cell proliferation, migration and invasion in oral squamous cell carcinoma by sponging miR-655-3p/FZD4 axis.

Recently, previous studies have shown that long non-coding RNA (lncRNA) can act as a tumor promoter or inhibitor in the pathogenesis of oral squamous cell carcinoma (OSCC). However, the regulatory mechanism of lncRNA SNHG5 is unknown in OSCC. Therefore, the functional mechanism of lncRNA SNHG5 in OSCC was initially revealed in this study. Here, RT-qPCR and western blot analysis were used to assess mRNA and protein expression. The functional mechanism of SNHG5 was investigated by MTT, Transwell and luciferase reporter assays. The results showed that SNHG5 expression was upregulated in OSCC and promoted the viability, migration and invasion of OSCC cells. In addition, SNHG5 is the sponge of miR-655-3p in OSCC. And miR-655-3p was found to play an inhibitory effect in OSCC by interacting with SNHG5. Moreover, miR-655-3p directly targets FZD4 and negatively regulates its expression in OSCC. Functionally, FZD4 promoted the progression of OSCC by interacting with the SNHG5/miR-655-3p axis. In conclusion, lncRNA SNHG5 promotes cell proliferation, migration and invasion in OSCC by regulating miR-655-3p/FZD4 axis.

[Impact of number of retrieved lymph nodes and lymph node ratio on the prognosis in patients with stage II and III colorectal cancer].

OBJECTIVE: To discuss the impact of number of retrieved lymph nodes and lymph node ratio(LNR) on the prognosis in patients with stage II and III colorectal cancer. METHODS: Clinicopathological data of 507 patients with stage II and III colorectal cancer were analyzed retrospectively. Follow-up was available in all the patients. RESULTS: The total number of retrieved lymph nodes was 5801, of which 1122 had metastasis. There was a positive correlation between metastatic lymph nodes and retrieved lymph nodes(r=0.171, P<0.01). In stage II colorectal cancer there was a significant difference in 5-year survival rate between patients with more than 12 lymph nodes retrieved and those with less than 12 lymph nodes retrieved(P<0.01). LNR also affected the 5-year survival rate of patients with stage II and III colorectal cancer(P<0.05). In patients with similar LNR, the 5-year survival rate differed significantly among different regions of lymph node metastasis(P<0.05). LNR influenced the prognosis independent of the number of lymph nodes retrieved. CONCLUSIONS: The number of retrieved lymph nodes is a prognostic factor for stage II and III colorectal cancer. More than 12 lymph nodes should be retrieved for better staging and prognosis. LNR is also a prognostic factor in stage II and III colorectal cancer. Regions of lymph nodes metastasis should be considered when evaluating the prognosis of patients using LNR.