[EPIZOOTOLOGIC MONITORING OF NATURAL-FOCAL INFECTIONS IN THE SOUTH OF RUSSIA IN 2015].

AIM: Analyze epizootologic manifestations of natural-focal infections (NFI) in the south of Russia in 2015. MATERIALS AND METHODS: Reports provided by administrations of Federal Service of Surveillance for Protection of Consumers Rights and Human Welfare, Centers of Hygiene and Epidemiology, Research Institutes for Plague Control and Station for Plague Control were used. Data processing was carried out using Excel program. RESULTS: A total of 37-738 samples of field material were studied, most of those - for tularemia (8268), Crimea hemorrhagic fever (6894) and West Nile fever (4875). Causative agents of 15 nosologic forms of NFI were detected during epizootologic examination of the territory of south of Russia, predominating amount of positive samples was obtained during study of material for tick-borne borreliosis (612), Crimea hemorrhagic fever (279) and tularemia (237). CONCLUSION: Analysis of epizootologic situation on natural- focal infections in the south of Russia gives evidence on the increase of activity of natural foci of bacterial (tularemia, tick-borne borreliosis), rickettsia (tick-borne spotted fevers) and viral infec- tions (Crimea hemorrhagic fever, West Nile fever, tick-borne viral encephalitis), and thus problems of prophylaxis of natural-focal infectious diseases and constant monitoring of natural foci in su- bjects of Southern, North-Caucasian and Crimea federal districts are of particular importance.

A NEW GENETIC VARIANT OF THE CRIMEAN-CONGO HEMORRHAGIC FEVER VIRUS ISOLATED IN CRIMEA.

This work represents the results of the genetic identification of the Crimean-Congo hemorrhagic fever virus (CCHF virus) strains isolated in the Crimean Federal District in conducting the epidemiological survey of the imported case of CCHF from Crimea in 2015. One sample of the serum from a patient and 61 pools (506 specimens) of ticks collected during the epizootiological survey of 6 administrative districts of the Crimean Federal District were tested using PCR for the presence of the CCHF virus RNA. RNA of the CCHF virus was detected in serum from a patient and 10 samples of ticks. The genetic identification of the CCHF virus was performed by sequencing the virus genome S-, M-, and L-segments. The result of the molecular-genetic analysis revealed a high degree of identity between the samples of the CCHF virus in human serum and three samples of ticks and their belonging to a new genetic Crimea subclade (Vd) of the genotype Europe 1. Whole genome sequencing of two samples of CCHF virus belonging to the Crimea subgroup (Vd) was performed. CCHF virus variants of the Crimea subclade (Vd) of the Europe- lgenotype were described for the first time. These variants were endemic to the territory of the Crimean peninsula.

[EPIDEMIOLOGIC SITUATION BY NATURAL-FOCI INFECTIONS IN THE CRIMEA FEDERAL DISTRICT IN 2014-2015].

AIM: Analysis of epidemic manifestations of natural-foci infections (NFI), clarification of spectrum of their causative agents, determination of epizootic activity of natural foci in the Crimea Federal District (KFD). MATERIALS AND METHODS: Epizootologic examination of 10 administrative districts of KDF was carried out. 291 pools (2705 specimens) of ixodes ticks and 283 samples of organs of small mammals were studied by PCRmethod for the presence of DNA/RNA of causative agents of a number of NFI. RESULTS: Morbidity by NFI in KFD was registered by 6 nosologies: Lyme borreliosis, Marseilles fever, leptospirosis; tularemia, intestine yersiniosis and tick-borne viral encephalitis, wherein, transmissive infections made up 91.6%. Circulation of causative agents of Crimea hemorrhagic fever, Q fever, group of tick-borne spotted fever, Lyme borreliosis, human granulocytic anaplasmosis, human monocytic ehrlichiosis, hemorrhagic fever with renal syndrome, West Nile fever, tularemia and leptospirosis was established. CONCLUSION: Due to activity of natural foci of NFI further monitoring of epidemiologic and epizootologic manifestations of these infections in the Crimea, including using genetic methods of analysis, is necessary for ensuring sanitary-epidemiologic welfare of KFD population.

[PROCUREMENT OF SANITARY AND EPIDEMIOLOGICAL WELFARE IN THE REGION OF-CITY-RESORT SOCHI FOR DANGEROUS AND NATURAL-FOCI INFECTIOUS DIS- EASES IN 2015].

AIM: Analysis ofresults of epidemiologic monitoring especially dangerous, natural-foci and other infectious diseases, asill as epizootologic activity of natural foci of infection on,the terri- tory, of city-resort Sochi. MATERIALS AND METHODS: Laboratory studies of 820. samples by PCR; im- mune- and bacteriologic methods were carried out, among those 344 - clinical material, 12 - water from open bodies and 321 - field material. Molecular-genetic identification of-143 strains of Vibrio-cholerae, isolated from open water bodies of city-resort Sochi, was-carried out,. RESULTS: Circulation of causative agents of Q fever, tularemia and hemorrhagic fever with renal syndrome of Dobrava-Adler genotypes was established, -as well as rickettsia of tick spotted fever group Predomination of rotaviruses (70.9%) was detected during study of samples of clinical material in etiologic structure of sporadically emerging acute intestine infections. Relatively high temperature values of river water during summer months (from 18, to 30 C), exit of sulfide mineral waters into the riverbed, that create-alkaline medium, seasonal reduction of river debit and speed of water current facilitated sustenance of contamination of water of Agura river by V.cholerae. CONCLUSION: Activity of natural foci of the infectious disease and contamination of Agura iver by V.cholerae Ο1, (atoxigenic) gives evidence on the necessity of constant control of epidemiologic situation by dangerous and natural-foci infectious diseases on the territory of city-resort Sochi, as well as study regional features, of their causative agents, including using genetic methods.

[Dysbiotic manifestations during eradication therapy of Helicobacter pylori and their corrections].

Forty-eight patients with duodenal peptic ulcer disease infected with Helicobacter pylori were examined. All patients undergo conventional 1-week eradication therapy. After its ending the patients were randomized to two groups: those who will be treated by synbiotics or control group. Normoflorin B and Normoflorin L, which contain bifidobacteria or lactobacilli in complex with different microelements, vitamins, aminoacids, organic acids, and antioxidants, were used as synbiotics. Morphologic study of biopsy samples of small intestine mucosa were performed in patients from both groups. It was determined that eradication therapy worsened existing symptoms of dyspepsia in 80.9% of cases or lead to their emergence, connected with dysbiotic manifestations, in 55.5% of patients. Inclusion of synbiotics in complex therapy resulted in rapid and effective elimination of dyspeptic symptoms, promoted recovery of affected morphologic and functional states of small intestine mucosal epithelium, and optimized metabolic processes important for the digestion.

[Hyalomma marginatum Koch in Stavropol' region]. / K izucheniiu Hyalomma marginatum Koch v Stavropol'skom krae.

Ixodes ticks of the species Hyalomma marginatum are widely spread in the semidesert and steppe landscape zones of the Stavropol Territory. The circle of the main hosts for the larvae and nymphs of these ticks includes many species of wild and domestic birds, European brown hare, four-toed and eared hedgehogs. Rodents are of no great importance for ticks as a feeding source. Adult ticks parasitize on agricultural animals, domestic dogs and attack humans. The prospects of further studies of the way of life this tick in connection with its importance for infectious pathology are discussed.

[Study of the natural sensitivity of Ixodes to insecticides]. / Izuchenie estestvennoi chuvsvitel'nosti iksodovykh kleshchei k nekotorym insektitsidam.

The article deals with the results obtained in the study of the natural sensitivity of the nature populations of Ixodes ticks to 4 insecticides. As revealed in this study, the ticks are highly sensitive to commercial preparations in different phases of their development. The selective sensitivity of different species of ticks to these preparations is noted.

[Role of some wild birds and mammals in the natural foci of Crimean haemorrhagic fever in Stavropol' region]. / Rol' nekotorykh dikikh ptits i mlekopitaiushchikh v prirodnoi ochagovosti krymskoi gemorragicheskoi likhoradki v Stavropol'skom krae.

The results of the study on the role of some wild mammals and birds as feeding sources of for ticks Hyalomma marginatum, the main vectors of Crimean haemorrhagic fever virus in the Stavropol Territory, in the preimago phases of their development are presented. These phases (larvae and nymphs) were found on rooks, hooded crows, partridges, European brown hares and eared hedgehogs. The examination of domestic fowl resulted in finding larvae and nymphs in small amounts on turkeys. According to the data of the epizootological survey carried out in summer and autumn of 2000 in the Stavropol Territory, rooks and, to a lesser extent, hares were found to be the main feeding sources for ticks in the preimago phases. Rodents seemed to be of minimal importance as feeding sources under the conditions of the Stavropol Territory. Of all animals, rooks must be the main object in the epizootological survey of the territory.

[Principles of predicting the density of rodent flea populations]. / Printsip prognoza plotnosti populiatsii blokh gryzunov.

The paper gives the indices of abundance of fleas of different species of rodents in their fur and in their burrows and lays emphasis on the general mechanisms by which the density of these blood-sucking insects is a species-specific (populational) sign and does not greatly differ from the mean many-year values. By using Citellophylus tesquorum and other specias as an example, the authors present the results of a study of natural mortality in fleas, which suggest that the mean longevity of imagoes and death in the preimaginal phases of development are a hereditary sign. An example of calculation of the death ratio "K" in the imaginal phases is given and evidence is provided for compiling the signs of the density of fleas in taking individual samples of a field material and calculating the abundance indices of specific species.