ABSTRACT
In the present study previously isolated Weissella cibaria CMG DEX3 capable of producing high molecular weight, water soluble dextran (Ahmed et al., 2012) is characterized for most efficient less expensive carbon, nitrogen sources, micro and macro nutrients by utilizing a multifactorial Placket-Burman statistical design for optimization of dextran production. A twelve run Plackett-Burman experimental model with slight modification was utilized to evaluate the impact of ten diverse nutrients on the production of dextran by the bacterial isolate Weissella cibaria CMG DEX3.
Subject(s)
Carbon/metabolism , Dextrans/biosynthesis , Leuconostocaceae/metabolism , Models, Statistical , Nitrogen/metabolism , Industrial Microbiology , Leuconostocaceae/isolation & purification , Molecular Weight , SolubilityABSTRACT
In the present study the potential of indigenous bacterial isolates from soil rhizosphere and marine environment to promote plant growth was determined. Eight bacterial strains isolated from soil and marine samples were characterized for the phosphate solubilizing activity. Qualitative and quantitative estimation of phosphate solubilization is done. MIC of antibiotic and heavy metals were checked for these strains. Strains show a diverse pattern of antibiotic and heavy metals resistance.
Subject(s)
Bacteria/metabolism , Phosphates/metabolism , Plants/microbiology , Seawater/microbiology , Soil Microbiology , Water Microbiology , Anti-Bacterial Agents/pharmacology , Bacteria/classification , Bacteria/drug effects , Bacteria/genetics , Bacteria/isolation & purification , Drug Resistance, Bacterial/genetics , Genetic Markers , Metals, Heavy/pharmacology , Microbial Sensitivity Tests , Plant Development , Plants/metabolism , Rhizosphere , Soil Pollutants/pharmacology , Solubility , Water Pollutants, Chemical/pharmacologyABSTRACT
Exopolysaccharide (EPS) producing Weissella cibaria CMGDEX3 was isolated from cabbage on sucrose containing De Man, Rogosa and Sharpe (MRS) agar. Dextransucrase activity and dextran yield was found to be 7.1 DSU ml(-1) and 2.4 g dl(-1), respectively. The structural characterization of purified EPS determined by FTIR, (1)H and (13)C NMR spectroscopy demonstrated that W. cibaria CMGDEX3 synthesized a linear dextran that predominately had α (1â6) glycosidic linkages with only a few (3.4%) α (1â3) linked branches. Molecular mass determination showed that it was a high molecular weight dextran of an average >2,000,000 Da. According to our knowledge this is the first report on isolation of dextran synthesizing Weissella genus from Pakistan.